pH SENSOR DYES pH SENSOR DYES p for Screening Antibody Internalization HAb Reactive Dyes are pH sensor dyes that have very low fluorescence at pH > 7 and a dramatic increase in fluorescence as the pH of the solution decreases. pHAb Dyes are designed specifically for antibody labeling and are available in two reactive forms suitable for antibody conjugations. A key feature of pHAb Dyes is the two sulfonate groups per dye, which increase solubility in water and reduce the aggregation often seen with non-sulfonated dyes. pHAb Dyes maintain their fluorescence response to pH change even after conjugation to antibody, as shown in Figure 1. • Accurately Determine Antibody Internalization: Fluorescence increases upon internalization, as pH is more acidic. • Use with On-bead Conjugation: Conjugate directly from biological samples expressing antibodies. • Compatible with Plate-based Assays: Measure internalization in 96-well plates, in real time. • Minimal Background Fluorescence: Only conjugated dyes are internalized and thus fluorescent; free dye outside of the cell and conjugated dye that is not internalized has little or no fluorescence (Figure 2). Learn More Pagina 5
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