pHAb Dyes fluoresce in response to acidic pH. 10,000 20,000 30,000 40,000 50,000 60,000 0 45 67 89 pH Figure 1. Both nonconjugated and Trastuzumab-conjugated pHAb Dyes fluoresce in response to acidic pH. The fluorescence of pHAb Dyes works well in plate-based assays. pHAb Amine Reactive Dye Trastuzumab-pHAb Amine Reactive Dye pHAb Thiol Reactive Dye Trastuzumab-pHAb Thiol Reactive Dye 60,000 50,000 40,000 30,000 20,000 10,000 0 SKBR3 TrastuzumabAmine-pHAb SKBR3 TrastuzumabThiol-pHAb Positive Samples MDA-MB231 TrastuzumabAmine-pHAb MDA-MB232 TrastuzumabThiol-pHAb SKBR3 RituximabAmine-pHAb Negative Controls Figure 2. SKBR3, HER2 positive cells and MDA-MB231, HER2 negative cells were plated in a 96-well black, clear bottom plate overnight. Trastuzumab, Rituximab, and Cituximab were conjugated with pHAb Amine Reactive Dye or pHAb Thiol Reactive Dye using on-bead conjugation. Rituximab and Cituximab served as negative control antibodies. enews | July 2015 SKBR3 CituximabAmine-pHAb Cell Type pHAb Dye Conjugate Fluorescence Fluorescence (RFU) 12903MA 13022MA Pagina 6
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