Science Catalog 2012 Life Cell Signaling Product EDTA, 0.5M (pH 8.0), Molecular Biology Grade Size Cat.# Price (Fr) EDTA, 0.5M (pH 8.0), Molecular Biology Grade For Research Use Only. Not for Use in Diagnostic Procedures. Description: EDTA, 0.5M (pH 8.0), Molecular Biology Grade, is a chelator of divalent cations and is suitable for biochemistry and molecular biology applications. It is supplied as a solution in deionized water. Form: Clear, colorless liquid. Properties: • pH at 25°C: 7.9–8.1. • A280 at 0.5M: ≤0.25. • RNase Activity at 0.5M: ≤1.0% release of 3H-RNA. • DNase Activity at 0.5M: ≤1.0% release of 3H-DNA. • Protease Assay: None detected. Storage Conditions: Store at 22–25°C. EDTA, Disodium Salt (Dihydrate), Molecular Biology Grade Product EDTA, Disodium Salt, Molecular Biology Grade For Research Use Only. Not for Use in Diagnostic Procedures. Description: EDTA, Disodium Salt, Molecular Biology Grade, is a chelator of divalent metal cations. Formula Weight: 372.20. Properties: • Purity: ≥99.0%. • Insolubles: ≤0.005%. • Lead: ≤5ppm. • Iron: ≤10ppm. Features: • Quality Tested: Each lot of EDTA is tested and certified to be free of DNase, RNase and protease activity. Storage Conditions: Store at 22–25°C. Product Ethidium Bromide Solution, Molecular Grade Size Conc. Ethidium Bromide Solution, Molecular Grade For Research Use Only. Not for Use in Diagnostic Procedures. Description: Ethidium Bromide Solution, Molecular Grade (10mg/ml), is a fluorescent dye suitable for staining nucleic acids after electrophoresis or in cesium chloride gradients. The solution can be used to detect both doublestranded and single-stranded DNA. Features: • Quality Tested: Each lot of Ethidium Bromide Solution is tested and certified to be free of DNase, RNase and protease activity. Storage Conditions: Store at 22–25°C. Glycerol, Molecular Biology Grade Product Glycerol, Molecular Biology Grade For Research Use Only. Not for Use in Diagnostic Procedures. Description: Glycerol is used for storage of enzymes at low temperatures. A 50% (w/v) glycerol solution will not freeze at –20°C. Glycerol is often used as a component in electrophoresis loading buffers because of its density (1.26g/ml). In addition, glycerol gradients can be used in the purification of bacteriophage or proteins. Cat.# H5433 is anhydrous glycerol with a purity of ≥99.5%. Cat.# Price (Fr) 10 ml 10 mg/ml H5041 66.00 Properties: • Purity: ≥99.5%. • Calcium: ≤2ppm. • Magnesium: ≤1ppm. • Lead: ≤5ppm. • Zinc: ≤1ppm. • A260 at 10%: ≤0.05. • A280 at 10%: ≤0.05. Features: • Quality Tested: Each lot of glycerol is tested and certified to be free of DNase, RNase and protease activity. Storage Conditions: Store at 22–25°C. Size Cat.# Price (Fr) 1,000 ml H5433 141.00 Size Cat.# Price (Fr) 100 g H5031 47.00 500 g H5032 177.00 100 ml V4231 33.00 400 ml V4233 106.00 Formamide, Molecular Grade Product Formamide, Molecular Grade For Research Use Only. Not for Use in Diagnostic Procedures. Description: Formamide is often used for the denaturation of nucleic acids in applications such as hybridization, sequencing gel electrophoresis and electron microscopy. Formula Weight: 45.04. Properties: • Purity: ≥99.5%. • Copper: ≤1ppm. • Iron: ≤1ppm. • Lead: ≤1ppm. • Zinc: ≤1ppm. • Refractive Index at 20°C: 1.446–1.448. • pH at 25°C of 1%: 6.5–7.5. • A260 at 10%: ≤0.10. • A280 at 10%: ≤0.02. Features: • Quality Tested: Each lot of Formamide is tested and certified to be free of DNase, RNase and protease activity. Storage Conditions: Store at 22–25°C. Size Cat.# Price (Fr) 100 ml H5051 47.00 500 ml H5052 177.00 6 For complete and up-to-date product information visit: www.promega.com/catalog Pagina 9
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