Cell Signaling Blue/Orange Loading Dye, 6X Product Blue/Orange Loading Dye, 6X For Laboratory Use. Size Cat.# Price (Fr) 3 ml G1881 80.00 Description: Blue/Orange Loading Dye, 6X, is a convenient marker dye containing 0.4% orange G, 0.03% bromophenol blue, 0.03% xylene cyanol FF, 15% Ficoll® 400, 10mM Tris-HCl (pH 7.5) and 50mM EDTA (pH 8.0). It is provided in a premixed, ready-to-use form. The dye is used for loading DNA samples into gel electrophoresis wells and tracking migration during electrophoresis. In a 0.5–1.4% agarose gel in 0.5X TBE, xylene cyanol FF migrates at approximately 4kb, bromophenol blue at approximately 300bp and orange G at approximately 50bp. Features: • Quality Tested: Each lot of Blue/Orange Loading Dye, 6X, is tested and certified to be free of nuclease activity. Storage Conditions: Store at –20°C. Boric Acid, Molecular Biology Grade (orthoboric acid) Product Boric Acid, Molecular Biology Grade For Research Use Only. Not for Use in Diagnostic Procedures. Description: Boric Acid, Molecular Biology Grade, in conjunction with Tris, is commonly used in buffers for the preparation of agarose or acrylamide gels and their associated running buffers. Formula Weight: 61.84. Properties: • Purity: ≥99.5%. • Iron: ≤5ppm. • Lead: ≤5ppm. • Moisture: ≤0.5%. • A260 at 1M: ≤0.015. • A280 at 1M: ≤0.010. Features: • Quality Tested: Each lot of Boric Acid is tested and certified to be free of DNase, RNase and protease activity. Storage Conditions: Store at 22–25°C. Product Bovine Serum Albumin, Acetylated Size Conc. Bovine Serum Albumin, Acetylated For Laboratory Use. Description: Bovine Serum Albumin, Acetylated, can be used as an enzyme stabilizer or as a carrier protein. It is prepared by a modification of the method of Gonzalez et al. and dialyzed extensively with deionized water to remove impurities. Features: • Quality Tested: Each lot of BSA is tested and certified to be free of DNase and RNase activity. Storage Conditions: Store at –20°C. Size Cat.# Price (Fr) 500 g H5001 45.00 1 kg H5003 81.00 Coelenterazines Product Coelenterazine Coelenterazine-h For Research Use Only. Not for Use in Diagnostic Procedures. Description: Luciferases from Renilla, Aequorea and other marine organisms are commonly used as indicators or reporters for studying cellular phenomena in expression assays in eukaryotic cells. Renilla luciferase is often used as a reporter of transcription regulation, whereas apoaequorin is often used as a calcium indicator. Other uses of coelenterazines include chemiluminescent detection of Reactive Oxygen Species (ROS) in cells or tissues. Promega offers the following coelenterazine analogs. Coelenterazine (native) is the luminescent substrate for Renilla luciferase and apoaequorin. Formula: C26H21N3O3. Formula Weight: 423.5. Form: Film. Coelenterazine-h imparts a luminescent intensity with its aequorin complex that is reported to be 10–20 times higher than that of native coelenterazine, making this derivative a useful tool for measuring small changes in Ca2+ concentrations. Formula: C26H21N3O2. Formula Weight: 407.5. Form: Film. Features: • Highly Pure: 95%. • Custom Capabilities: Custom packaging and sizes available. • Easy to Prepare: Supplied as a dried substrate for easy preparation in methanol or ethanol. • Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/myway/ Storage Conditions: Store at –20°C. DTT, Molecular Grade (DL-Dithiothreitol) Product DTT, Molecular Grade DTT, Molecular Grade (Dry Powder) Size Conc. Cat.# Price (Fr) 100 µl 100 mM P1171 48.00 5 g 25 g P1171 For Laboratory Use. V3151, V3155 For Research Use Only. Not for Use in Diagnostic Procedures. Description: DTT, Molecular Grade, is an antioxidant used to stabilize enzymes and other proteins containing sulfhydryl groups. The liquid form of the product is a 100mM solution of DTT in water. Cat.# Price (Fr) 1 ml 10 mg/ml R3961 58.00 400 µl 1 µg/µl R9461 42.00 Formula: C4H10O2S2. Formula Weight: 154.25. Form: White crystals/powder or liquid in deionized water. Properties: • Purity: ≥99.0%. • Melting Point: 40–44°C. • A283 at 20mM: ≤0.04. • % Oxidized: ≤0.50%. Features: • Quality Tested: Each lot of DTT is tested and certified to be free of DNase, RNase and protease activity. Storage Conditions: Store at –20°C. V3151 133.00 V3155 483.00 Size Cat.# Price (Fr) 250 µg S2001 180.00 250 µg S2011 180.00 1 For complete and up-to-date product information visit: www.promega.com/catalog 5 Biochemicals and Labware Pagina 8
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