Science Catalog 2012 Life Cell Signaling Antibiotic G-418 Sulfate Product Antibiotic G-418 Sulfate Antibiotic G-418 Sulfate Solution For Research Use Only. Not for Use in Diagnostic Procedures. Description: Antibiotic G-418 Sulfate is an aminoglycosidic antibiotic toxic to both prokaryotic and eukaryotic cells. It acts by interfering with protein synthesis and is used as an agent for selection of cultured cells expressing a gene (i.e., aminoglycoside 3´ phosphotransferase [APH 3´]) that confers resistance to G-418. The liquid form of the product is in distilled water and aseptically filtered. Formula Weight: 692.6 (anhydrous). Form: White powder. Physical/Chemical Properties of Powder: • Appearance: White powder. • TLC: Single major spot. • Elemental Analysis: %C = 28.8–36.07; %H = 5.76–7.76; %N = 6.72–8.41. • Absorbance: A280 (1mg/ml) = 0–0.015; A570 (100mg/ml) = 0–0.1. • Specific Rotation: +104° to +121°. Properties Specific to V7981, V7982, V7983: • Appearance: White powder. • Hydration Waters: 0–6, as determined from Elemental Analysis. • Potency: >500μg/mg. Properties Specific to V8091: • Potency: 40–60mg/ml. • Sterility: Aseptically filtered. Features: • Sterile: Antibiotic G-418 Sulfate Solution is quality tested for sterility. Storage Conditions: Store powder at 22–25°C. Store liquid at –20°C. BCIP/NBT Color Development Substrate (5-bromo-4-chloro-3-indolyl-phosphate/nitro blue tetrazolium) Product BCIP/NBT Color Development Substrate For Laboratory Use. Size Cat.# Price (Fr) 1.25/2.5 ml S3771 126.00 Description: BCIP (5-bromo-4-chloro-3-indolyl-phosphate) is used in conjunction with NBT (nitro blue tetrazolium) for the colorimetric detection of alkaline phosphatase activity. Each vial of BCIP is supplied with a vial of NBT. Preparation of Substrates to Detect Alkaline Phosphatase: For every 5ml of alkaline phosphatase buffer (100mM Tris-HCl [pH 9.0], 150mM NaCl, 1mM MgCl2), add 33μl NBT and 16.5μl BCIP. Add the NBT first, mix, add the BCIP, and mix again. Use within 1 hour, and discard any unused solution. Concentration: BCIP (50mg/ml) in 100% dimethylformamide; NBT (50mg/ml) in 70% dimethylformamide. Features: • Quality Tested: Each lot of BCIP/NBT Color Development Substrate is tested and qualified for use in blotting. Storage Conditions: Store at either 4°C or –20°C. Size Cat.# Price (Fr) 100mg V7981 51.00 1g V7982 153.00 5g V7983 421.00 20ml V8091 130.00 Beetle Luciferin, Potassium Salt Product Beetle Luciferin, Potassium Salt Size Cat.# Price (Fr) 5 mg E1601 80.00 50 mg E1602 400.00 250 mg E1603 800.00 1 g E1605 Pls. Enq. For Research Use Only. Not for Use in Diagnostic Procedures. Description: Luciferase genes from the North American firefly (Photinus pyralis) and from other beetles are commonly used as reporter genes for studying transcription regulation in transient assay systems and as markers for stably transformed eukaryotic cells. Beetle luciferin (also known as D-luciferin) is synthesized as the monopotassium salt and is a substrate for the beetle luciferase reporter systems. D-luciferin is provided for those researchers who prefer to formulate their own assay reagents for monitoring in vitro or in vivo luciferase activity. Formula: C11H7N2O3S2•K. Formula Weight: 318.4Da (anhydrous). Features: • Formulation: Supplied as a potassium salt for easy preparation in aqueous buffer. • Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/myway/ Storage Conditions: Store at –70°C. Bisacrylamide, Molecular Grade (N,N´Methylenebisacrylamide) Product Bisacrylamide, Molecular Grade For Research Use Only. Not for Use in Diagnostic Procedures. Description: Bisacrylamide, Molecular Grade, is a cross-linking agent used in the preparation of polyacrylamide gels. This product is tested for its efficiency in gel polymerization. Formula Weight: 154.20. Form: White, free-flowing crystals. Properties: • Purity: ≥99.0%. • Acrylic Acid (CH2:CHCOOH): ≤0.001%. • A290 (1% solution): ≤0.20. • Magnesium: ≤2ppm. • Conductivity (2% in water): ≤10μmhos. Features: • Quality Tested: Each lot of Bisacrylamide is tested and certified to be free of DNase, RNase and protease activity. Storage Conditions: Store at 22–25°C. Size Cat.# Price (Fr) 25 g V3141 49.00 125 g V3143 172.00 4 For complete and up-to-date product information visit: www.promega.com/catalog Pagina 7
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