Cell Signaling Untagged Flexi® Mammalian Expression Vectors Product pF4A CMV Flexi® Vector pF4K CMV Flexi® Vector pF5A CMV-neo Flexi® Vector pF5K CMV-neo Flexi® Vector pF9A CMV hRluc-neo Flexi® Vector For Research Use Only. Not for Use in Diagnostic Procedures. Description: These Flexi® Vectors are designed specifically for high-level expression of proteins in mammalian cells from the CMV promoter with or without a selectable marker. The pFN9A Vector provides Renilla luciferase, which may be used as a transfection control. The pFN9A Vector was designed to complement pGL4 firefly luciferase vectors when exogenous proteins (e.g., a receptor of transcription factor) must be expressed for reporter assays. All inserts may be confirmed by cell-free expression with the TN System (Cat.# L1170). T® T7 Quick Note: Flexi® Vectors contain the lethal barnase gene to reduce background colonies without inserts during the subcloning procedure. Using the Flexi® Vector Cloning System replaces the barnase gene with your insert. These vectors, as purchased, cannot be cultured in normal laboratory strains of E. coli without an insert. Features: • Versatility: You can choose between a variety of initial applications (e.g., bacterial protein, mammalian, or cell-free protein expression) and then transfer to others as required. • Time Savings: Efficient transfer allows for direct use of recombinant clones, minimizing time wasted screening background colonies. • Enhanced Productivity: Adaptable to high-throughput formats for large screening projects. • Easy Access: No licensing fees or complicated transfer restrictions. Storage Conditions: Store vectors at –20°C. Inducible T7-Driven Flexi® Vectors for E. coli Expression Product pF1A T7 Flexi® Vector pF1K T7 Flexi® Vector pFN18A HaloTag® T7 Flexi® Vector pFN18K HaloTag® T7 Flexi® Vector pFN19A HaloTag® T7 SP6 Flexi® Vector pFN19K HaloTag® T7 SP6 Flexi® Vector pFC20A HaloTag® T7 SP6 Flexi® Vector pFC20K HaloTag® T7 SP6 Flexi® Vector pFN2A (GST) Flexi® Vector pFN2K (GST) Flexi® Vector pFN6A (HQ) Flexi® Vector pFN6K (HQ) Flexi® Vector pFC7A (HQ) Flexi® Vector pFC7K (HQ) Flexi® Vector For Research Use Only. Not for Use in Diagnostic Procedures. For additional information see page 239. Size Cat.# Price (Fr) 20 µg C8441 424.00 20 µg C8451 424.00 20 µg G2751 399.00 20 µg G2681 399.00 20 µg G1891 399.00 20 µg G1841 399.00 20 µg G1681 399.00 20 µg G1691 399.00 20 µg C8461 476.00 20 µg C8471 476.00 20 µg C8511 476.00 20 µg C8521 476.00 20 µg C8531 476.00 20 µg C8541 476.00 Size Cat.# Price (Fr) 20 µg C8481 476.00 20 µg C8491 476.00 20 µg C9401 476.00 20 µg C9411 476.00 20 µg C9361 476.00 pALTER®-MAX Vector Product pALTER®-MAX Vector Available Separately Ampicillin Repair Oligonucleotide For Research Use Only. Not for Use in Diagnostic Procedures. Description: The pALTER®-MAX Vector is a 5,534bp plasmid. It contains the human cytomegalovirus (CMV) immediate-early enhancer/promoter region for strong, constitutive expression of cloned DNA inserts in a variety of mammalian cell types. The pALTER®-MAX Vector as supplied is chloramphenicol-resistant and ampicillin-sensitive. Features: • High Expression: Protein expression driven by the human cytomegalovirus (CMV) immediate-early enhancer/promoter region allows high constitutive expression in a variety of cell lines. A chimeric intron composed of the 5´-splice site from the β-globin intron and the 3´-splice site from an IgG intron allows increased expression levels. • Efficient: SV40 late polyadenylation signal for efficient RNA processing. T7 and T3 RNA polymerase promoters flank the multiple cloning site and allow RNA in vitro synthesis from sense or antisense strands. • Flexible: Restriction sites flanking the CMV enhancer (BglII and SgfI) and the CMV promoter (SgfI and I-PpoI) allow for easy replacement of these regions by alternative regulatory regions. Storage Conditions: Store vector DNA at –20°C. pGEM®-3Z Vector Product pGEM®-3Z Vector For Research Use Only. Not for Use in Diagnostic Procedures. Description: The pGEM®-3Z Vector is intended for use as a standard cloning vector, as well as for the highly efficient synthesis of RNA in vitro. The vector carries the lacZ α-peptide and the multiple cloning region arrangement from pUC18 allowing selection of recombinants by blue/white screening. In addition, the vector contains both the SP6 and T7 RNA polymerase promoters flanking the multiple cloning region. The pGEM®-3Z and pGEM®-4Z Vectors are essentially identical except for the orientation of the SP6 and T7 promoters. Features: • Blue/White Screening: Allows the easy identification of recombinant clones. • Versatile: This vector can be used for standard cloning and in vitro transcription from SP6 and T7 RNA polymerase promoters flanking the multiple cloning region. • Convenient: Multiple cloning site provides a selection of restriction sites for cloning. Storage Conditions: Store vector at –20°C. Protocol pGEM®-3Z Vector Technical Bulletin XmnI 1937 ScaI 1818 Ampr pGEM®-3Z Vector (2,743bp) SspI 2142 AatII 2260 NdeI 2509 T7 NarI 2561 lacZ EcoRI SacI KpnI AvaI XmaI SmaI BamHI XbaI SalI AccI HincII PstI SphI ori HindIII SP6 15 21 21 21 23 26 32 38 39 40 48 54 56 69 1 start 5 Part# TB033 Size Cat.# Price (Fr) 20 µg P2151 186.00 Size Cat.# Price (Fr) 20 µg Q5761 523.00 30 µl Q6311 97.00 4 For complete and up-to-date product information visit: www.promega.com/catalog 99 Cloning and DNA Markers 0278VA05_4A Pagina 102
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