Science Catalog 2012 Life Cell Signaling pGEM®-3Zf(+/–) Vectors Product pGEM®-3Zf(+) Vector pGEM®-3Zf(–) Vector For Research Use Only. Not for Use in Diagnostic Procedures. Description: The pGEM®-3Zf(+) and pGEM®-3Zf(–) Vectors are derived from the pGEM®-3Z Vector and contain the origin of replication of the filamentous phage f1. These plasmids contain T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the α-peptide coding region of β-galactosidase. Insertional inactivation of the α-peptide allows recombinant clones to be identified directly by color screening on indicator plates when using appropriate E. coli strains (e.g., JM109). The multiple cloning region contains unique restriction sites for EcoRI, SacI, KpnI, AvaI, SmaI, BamHI, XbaI, SalI, AccI, HincII, PstI, SphI and HindIII. The pGEM®-3Zf(+) and -3Zf(–) Vectors are identical except for the orientation of the f1 origin and can be used as standard cloning vectors, as templates for in vitro transcription and for the production of circular ssDNA. Features: • Blue/White Screening: Allows the easy identification of recombinant clones. • Versatile: These vectors can be used for standard cloning, single-stranded DNA production and in vitro transcription from SP6 and T7 RNA polymerase promoters flanking the multiple cloning region. • Convenient: Multiple cloning site provides a selection of restriction sites for cloning. Storage Conditions: Store vector at –20°C and bacterial strain at –70°C. Protocol pGEM®-3Zf(+) Vector Technical Bulletin pGEM®-3Zf(–) Vector Technical Bulletin AatII 2260 XmnI 1937 ScaI 1818 Ampr NdeI 2509 T7 f1 ori pGEM®-3Zf(+/–) Vectors (3,197bp) lacZ EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII PstI SphI ori HindIII SP6 15 21 21 23 26 32 38 39 40 48 54 56 69 1 start 5 ori Part# TB086 TB045 Ampr pGEM®-4Z Vector (2,746bp) Size Cat.# Price (Fr) 20 µg P2271 186.00 20 µg P2261 186.00 pGEM®-4Z Vector Product pGEM®-4Z Vector For Research Use Only. Not for Use in Diagnostic Procedures. Description: The pGEM®-4Z Vector is intended for use as a standard cloning vector, as well as for the highly efficient synthesis of RNA in vitro. The vector carries the lacZ α-peptide and the multiple cloning region arrangement from pUC18 allowing selection of recombinants by blue/white screening. In addition, the vector contains both the SP6 and T7 RNA polymerase promoters flanking the multiple cloning region. The pGEM®-3Z and pGEM®-4Z Vectors are essentially identical except for the orientation of the SP6 and T7 promoters. Features: • Blue/White Screening: Allows the easy identification of recombinant clones. • Versatile: This vector can be used for standard cloning and in vitro transcription from SP6 and T7 RNA polymerase promoters flanking the multiple cloning region. • Convenient: Multiple cloning site provides a selection of restriction sites for cloning. Storage Conditions: Store vector at –20°C and bacterial strain at –70°C. Protocol pGEM®-4Z Vector Technical Bulletin XmnI 1939 ScaI 1820 SspI 2144 AatII 2262 NdeI 2511 NarI 2563 lacZ SP6 EcoRI SacI KpnI AvaI XmaI SmaI BamHI XbaI SalI AccI HincII PstI SphI HindIII T7 17 23 23 23 25 28 34 40 41 42 50 56 58 70 1 start 7 Part# TB036 Size Cat.# Price (Fr) 20 µg P2161 186.00 100 For complete and up-to-date product information visit: www.promega.com/catalog 0282VA05_4A 0280VA05_4A Pagina 103
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