Cell Signaling pGEM®-5Zf(+) Vector Product pGEM®-5Zf(+) Vector For Research Use Only. Not for Use in Diagnostic Procedures. Description: The pGEM®-5Zf(+) Vector is derived from the pGEM®-3Zf(+) Vector and contains the origin of replication of the filamentous phage f1. This plasmid serves as a standard cloning vector, as a template for in vitro transcription and can be used for the production of circular ssDNA. This vector contains T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the α-peptide coding region of β-galactosidase. Insertional inactivation of the α-peptide allows recombinant clones to be identified directly by color screening on indicator plates when using appropriate E. coli strains (e.g., JM109). The multiple cloning region contains unique restriction sites for ApaI, AatII, SphI, NcoI, SacII, EcoRV, SpeI, NotI, PstI, SalI, NdeI, SacI, BstXI and NsiI. This arrangement is designed specifically for generating unidirectional deletions with the Erase-a-Base™ System. Features: • Blue/White Screening: Allows the easy identification of recombinant clones. • Versatile: This vector can be used for standard cloning, single-stranded DNA production and in vitro transcription from SP6 and T7 RNA polymerase promoters flanking the multiple cloning region. • Convenient: Multiple cloning site provides a selection of restriction sites for cloning. • Unidirectional Deletions: Restriction sites are positioned conveniently for use with the Erase-a-Base™ System. Storage Conditions: Store vector at –20°C and bacterial strain at –70°C. Protocol pGEM®-5Zf(+) Vector Technical Bulletin XmnI 1994 ScaI 1875 f1 ori Ampr pGEM®-5Zf(+/–) Vectors (3,000bp) T7 lacZ ApaI AatII SphI NcoI SacII EcoRV SpeI NotI PstI SalI NdeI SacI BstXI NsiI ori SP6 14 103 112 126 20 26 37 46 51 55 62 73 75 82 94 1 start XmnI 1991 ScaI 1872 f1 ori Ampr pGEM®-7Zf(+/–) Vectors (2,997bp) lacZ T7 ApaI AatII SphI XbaI XhoI EcoRI KpnI SmaI Csp45I ClaI HindIII BamHI SacI BstXI NsiI ori SP6 1 start 100 109 14 20 26 31 37 43 53 56 61 67 72 78 91 123 Part# TB047 Size Cat.# Price (Fr) 20 µg P2241 238.00 pGEM®-7Zf(+/–) Vectors Product pGEM®-7Zf(+) Vector pGEM®-7Zf(–) Vector For Research Use Only. Not for Use in Diagnostic Procedures. Description: The pGEM®-7Zf(+) and pGEM®-7Zf(–) Vectors are derivatives of the pGEM®-3Zf(+) Vector and contain the origin of replication of the filamentous phage f1. These plasmids serve as standard cloning vectors, as templates for in vitro transcription and can be used for the production of circular ssDNA. These plasmids contain SP6 and T7 RNA polymerase promoters flanking a region of multiple cloning sites within the α-peptide coding region of β-galactosidase. Insertional inactivation of the α-peptide allows recombinant clones to be identified directly by color screening on indicator plates when using appropriate E. coli strains (e.g., JM109). The multiple cloning region is unique and includes restriction sites for ApaI, AatII, SphI, XbaI, XhoI, EcoRI, KpnI, SmaI, Csp45I, ClaI, HindIII, BamHI, SacI, BstXI and NsiI. This arrangement is designed specifically for generating unidirectional deletions with the Erase-a-Base™ System. pGEM®-7Zf(+) and pGEM®-7Zf(–) Vectors are identical except for the orientation of the f1 origin. Features: • Blue/White Screening: Allows the easy identification of recombinant clones. • Versatile: These standard cloning vectors are equipped for singlestranded DNA production and in vitro transcription from SP6 and T7 RNA polymerase promoters flanking the multiple cloning region. • Convenient: Multiple cloning site provides a selection of restriction sites for cloning. • Unidirectional Deletions: Restriction sites are positioned conveniently for use with the Erase-a-Base™ System. Storage Conditions: Store vector at –20°C and bacterial strain at –70°C. Protocol pGEM®-7Zf(+) Vector Technical Bulletin pGEM®-7Zf(–) Vector Technical Bulletin Part# TB048 TB069 Size Cat.# Price (Fr) 20 µg P2251 238.00 20 µg P2371 238.00 4 For complete and up-to-date product information visit: www.promega.com/catalog 101 Cloning and DNA Markers 0284VA05_4A 0286VA05_4A Pagina 104
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