Science Catalog 2012 Life Cell Signaling pGEM®-9Zf(–) Vector Product pGEM®-9Zf(–) Vector For Research Use Only. Not for Use in Diagnostic Procedures. Description: The pGEM®-9Zf(–) Vector is a recombinant plasmid designed to provide a versatile range of cloning strategies, efficient synthesis of RNA in vitro and the production of single-stranded DNA. The plasmid contains T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the α-peptide coding region of β-galactosidase. Insertional inactivation of the α-peptide allows recombinant clones to be identified directly by color screening on indicator plates when using appropriate E. coli strains (e.g., JM109). The multiple cloning region is unique and includes restriction sites for NsiI, SpeI, HindIII, XbaI, EcoRI, SalI and SacI. Features: • Excisable SP6/T7 Insert: This vector allows the excision of an insert containing the SP6 and T7 RNA polymerase promoters. • Blue/White Screening: Allows the easy identification of recombinant clones. • Versatile: This vector can be used for standard cloning, single-stranded DNA production and in vitro transcription from SP6 and T7 RNA polymerase promoters flanking the multiple cloning region. • Convenient: Multiple cloning site provides a selection of restriction sites for cloning. Storage Conditions: Store vector at –20°C and bacterial strain at –70°C. Protocol pGEM®-9Zf(–) Vector Technical Bulletin XmnI 1935 ScaI 1816 f1 ori Ampr pGEM®-9Zf(–) Vector (2,912bp) lacZ NaeI 2382 Tth111I NotI SP6 NsiI SpeI HindIII XbaI EcoRI SalI SacI SfiI ori ori 2879 2887 T7 55 82 12 18 27 36 42 52 1 start 9 XmnI 1961 ScaI 1842 Ampr f1 ori pGEM®-11Zf(+/–) Vectors (3,221bp) AatII 2284 NdeI 2533 Part# TB070 Size Cat.# Price (Fr) 20 µg P2391 238.00 pGEM®-11Zf(+/–) Vectors Product pGEM®-11Zf(+) Vector pGEM®-11Zf(–) Vector For Research Use Only. Not for Use in Diagnostic Procedures. Description: The pGEM®-11Zf(+) and pGEM®-11Zf(–) Vectors can be used as standard cloning vectors, as templates for in vitro transcription and for the production of ssDNA. These plasmids contain T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the α-peptide coding region of β-galactosidase. Insertional inactivation of the α-peptide allows recombinant clones to be identified directly by color screening on indicator plates when using appropriate E. coli strains (e.g., JM109). The multiple cloning region contains unique restriction sites for SfiI, SacI, EcoRI, SalI, XhoI, BamHI, ApaI, XbaI, NotI, SphI, NsiI and HindIII. The pGEM®-11Zf(–) and pGEM®-11Zf(+) Vectors are identical except for the orientation of the f1 origin. Features: • Blue/White Screening: Allows the easy identification of recombinant clones. • Versatile: These vectors can be used for standard cloning, single-stranded DNA production and in vitro transcription from SP6 and T7 RNA polymerase promoters flanking the multiple cloning region. • Convenient: Multiple cloning site provides a selection of restriction sites for cloning. Storage Conditions: Store vector at –20°C and bacterial strain at –70°C. Protocol pGEM®-11Zf(+) Vector Technical Bulletin pGEM®-11Zf(–) Vector Technical Bulletin Part# TB075 TB074 Size Cat.# Price (Fr) 20 µg P2411 238.00 20 µg P2421 238.00 T7 SfiI lacZ SacI EcoRI SalI XhoI 1 start BamHI ApaI XbaI NotI SphI NsiI HindIII SP6 17 27 29 35 41 47 57 59 67 76 78 80 93 102 For complete and up-to-date product information visit: www.promega.com/catalog    0288VA05_4A  0290VA05_4A Pagina 105

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