Cell Signaling pSP64 Poly(A) Vector Product pSP64 Poly(A) Vector For Research Use Only. Not for Use in Diagnostic Procedures. Description: The pSP64 Poly(A) Vector can be used as a standard cloning vector and for in vitro transcription from the SP6 promoter. The pSP64 Poly(A) Vector also can be used to generate poly(A)+ transcripts in vitro. The vector has a stretch of 30 dA:dT residues inserted between the SacI and EcoRI sites. Therefore, when foreign DNA is cloned into any polylinker site other than EcoRI (HindIII, PstI, SalI, AccI, HincII, XbaI, BamHI, AvaI, SmaI or SacI), linearization of the recombinant plasmid with EcoRI allows the use of SP6 RNA polymerase in vitro to prepare RNA copies of the inserted sequences that contain a synthetic 3´ “poly(A)” tail of 30 residues. Features: • In Vitro Transcription: The SP6 promoter is next to the polylinker. • Generates Poly(A)+ Transcripts In Vitro: A stretch of 30 dA:dT residues are inserted between the SacI and EcoRI sites in the polylinker. Poly(A) tails can stabilize RNAs and lead to greater yields for in vitro translation reactions. • Convenient: Multiple cloning region provides a selection of restriction sites for cloning. Storage Conditions: Store vector at –20°C. Protocol pSP64 Poly(A) Vector Technical Bulletin SspI 2138 XmnI 1933 ScaI 1814 PvuI 1704 FspI 1556 BglI 1454 Ampr pSP64 Poly(A) Vector (3,030bp) PvuII 265 ori AatII 2256 SphI 2599 NaeI 2758 NheI 2789 SP6 HindIII PstI SalI AccI HincII XbaI BamHI AvaI SmaI SacI (dA:dT) 30 EcoRI 20 22 23 24 28 34 39 41 50 86 1 start 7 Part# TB052 FspI 430 BglI 1328 Size Cat.# Price (Fr) 20 µg P1241 170.00 pSP72 Vector Product pSP72 Vector For Research Use Only. Not for Use in Diagnostic Procedures. Description: The pSP72 Vector can be used as a standard cloning vector and also can be used for transcription of RNA in vitro. The pSP72 Vector contains the SP6 and T7 RNA polymerase promoters flanking a unique multiple cloning region, which includes restriction sites for XhoI, PvuII, HindIII, SphI, PstI, SalI, AccI, XbaI, BamHI, SmaI, KpnI, SacI, EcoRI, ClaI, EcoRV and BglII. The pSP72 and pSP73 Vectors are essentially identical except for the orientation of the multiple cloning site region. Features: • Versatile: This vector can be used for standard cloning and in vitro transcription from SP6 and T7 RNA polymerase promoters flanking the multiple cloning region. • Convenient: Multiple cloning site provides a selection of restriction sites for cloning. Storage Conditions: Store vector at –20°C. Protocol pSP72 Vector Technical Bulletin ScaI 1688 PvuI 1578 Ampr (2,462bp) pSP72 Vector XmnI 1807 SspI 2012 AatII 2130 NdeI 2379 SP6 XhoI PvuII HindIII SphI PstI SalI AccI XbaI ori HpaI 136 BamHI SmaI KpnI SacI EcoRI ClaI EcoRV BglII T7 12 16 26 32 34 35 40 46 53 59 65 67 74 81 85 101 1 start 4 Part# TB040 Size Cat.# Price (Fr) 20 µg P2191 186.00 4 For complete and up-to-date product information visit: www.promega.com/catalog 103 Cloning and DNA Markers 0306VA05_2A 0298VA05_2A Pagina 106
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