Science Catalog 2012 Life Cell Signaling Wizard® SV 96 PCR Clean-Up System Product Wizard® SV 96 PCR Clean-Up System Size Cat.# Price (Fr) 1 × 96 preps A9340 244.00 4 × 96 preps A9341 854.00 8 × 96 preps A9342 1623.00 100 × 96 preps A9345 Pls. Enq. Available Separately Membrane Binding Solution Wizard® SV 96 Binding Plates For Research Use Only. Not for Use in Diagnostic Procedures. Description: The Wizard® SV 96 PCR Clean-Up System is designed for highthroughput purification of 100bp to 10kb PCR products from excess nucleotides, primers and primer dimers. This membrane-based system recovers >90% PCR fragments in as little as 20 minutes. The purified DNA can be used for automated fluorescent sequencing, cloning, labeling, restriction digestion or microarray analysis without further manipulation. The Wizard® SV 96 PCR Clean-Up System uses 96-well filtration without the need to disassemble the manifold. Filtrate waste is delivered directly to a vacuum trap, eliminating the need to dispose of collected waste within the manifold assembly. Protocols are available for automated instruments from Beckman Coulter and PerkinElmer. Features: • High Performance: Optimized methods deliver purified PCR products suitable for demanding applications such as microarray analysis. • Confidence: Average recovery for 100–500bp fragments of >90%. Automated fluorescent sequencing Phred* 20 scores >600. • Automation: Validated automated methods available at: www.promega.com/automethods/ • Your Choice of Configuration: Learn more about our custom options for this product at: www.promega.com/myway/ *A Phred score is a widely recognized method to measure the quality of DNA sequences. Phred is a base-calling program for DNA sequence traces available from Codoncode Corporation. Storage Conditions: Store at 22–25°C. Protocol Wizard® SV 96 PCR Clean-Up System Technical Bulletin Part# TB311 20 ml A9301 112.00 10 pack A2271 1381.00 A. + Betaine – Betaine UP PP PU PP PP B. 1. 2. Microarray of purified PCR products. PCR products (300bp) were amplified in the presence or absence of 1M betaine, then purified using the Wizard® SV 96 PCR Clean-Up System. Panel A. Agarose gel analysis. Purified (P) and unpurified (U) PCR products amplified with (+) or without (–) betaine were separated on an ethidium bromide-stained, 2% agarose gel. Panel B. Representative microarray blocks of purified PCR product hybridized to complementary Cy®-labeled cDNA. Betaine interferes with microarray analysis, so the fact that the microarray data for PCR with and without betaine are equivalent clearly demonstrates removal of betaine using the Wizard® SV 96 PCR Clean-Up System. 1. PCR product amplified under standard amplification conditions (–betaine). 2. 1M betaine added to the PCR mix. 108 For complete and up-to-date product information visit: www.promega.com/catalog 3796TA08_2A Pagina 111
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