Cell Signaling Wizard® MagneSil® Sequencing Reaction Clean-Up System Product Wizard® MagneSil® Sequencing Reaction Clean-Up System Wizard® MagneSil® Sequencing Reaction Clean-Up System, HTP1 Available Separately MagneSil® GREEN For Research Use Only. Not for Use in Diagnostic Procedures. Description: The Wizard® MagneSil® Sequencing Reaction Clean-Up System was developed for high-throughput purification of sequencing reactions, including BigDye® Terminator reactions. Cleanup is performed using the proprietary MagneSil® GREEN Paramagnetic Particles with standard, nonskirted 96-well amplification plates. No user intervention is required from the time the plates are placed on the instrument until the samples are ready for loading onto the fluorescent DNA sequencer. Protocols are available for automated instruments from Beckman Coulter and Tecan. The system relies upon the MagnaBot® II for magnetic separation. The Plate Clamp 96 and Plate Stand are recommended for automated use because they ensure PCR plates are uniformly flat for liquid transfer on a robotic instrument. Features: • Get Immediate Results: Validated, walkaway method. • Gain Confidence in Results: Purified products are approved for fluorescent sequencing reactions. Phred* 20 quality scores ≥650 bases. • Automate This Assay: Validated automated methods available at: www.promega.com/automethods/ *A Phred score is a widely recognized method to measure the quality of DNA sequences. Phred is a base-calling program for DNA sequence traces available from Codoncode Corporation. Storage Conditions: Store at 22–25°C. Protocol Wizard® MagneSil® Sequencing Reaction Clean-Up System Technical Bulletin Part# TB287 Electropherogram of purified sequencing reactions analyzed on an ABI PRISM® 3700 DNA Sequencer. BigDye® terminator reactions purified with the Wizard® MagneSil® Sequencing Reaction Clean-Up System. Size Cat.# Price (Fr) 4 × 96 preps A1831 802.00 8 × 96 preps A1832 1524.00 100 × 96 preps A1835 Pls. Enq. 100 ml A8231 574.00 5 Cleanup is performed using the MagnaBot® II Magnetic Separation Device (Cat.# V8351) accompanied by the Plate Clamp 96 (Cat.# V8251). These devices are designed to work with most robotic platforms. For complete and up-to-date product information visit: www.promega.com/catalog 109 DNA and RNA Purification 3250CC03_1A 3312TA03_1A Pagina 112

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