Science Catalog 2012 Life Cell Signaling Genomic DNA Purification ReliaPrep™ Large Volume HT gDNA Isolation System Product ReliaPrep™ Large Volume HT gDNA Isolation System Available Separately ReliaPrep™ LV 32 HSM Instrument ReliaPrep™ LV 32 HSM Standard Service Agreement ReliaPrep™ Cover ReliaPrep™ Tube Rack ReliaPrep™ Tube Rack Stand 1 each A1715 Pls. Enq. 1 each SA3070 Pls. Enq. 1 each A1712 180.00 1 each A1713 416.00 1 each A1714 304.00 A1751 For Laboratory Use. A1715, SA3070, A1712, A1713, A1714 For Research Use Only. Not for Use in Diagnostic Procedures. Product may not be available in all countries. Please contact your local representative for more information. Description: The ReliaPrep™ Large Volume HT gDNA Isolation System isolates genomic DNA (gDNA) from blood samples ranging from 3ml to 10ml of blood in a scalable format. The chemistry eliminates tedious centrifugation steps as well as the use of hazardous chemicals, which are inherent in precipitation-based chemistries. The system has been automated on robotic liquid-handling workstations, allowing walkaway purification of genomic DNA from 3–10ml of whole blood, regardless of sample storage or shipping conditions. For low-throughput isolation of gDNA from up to 32 samples at one time, the ReliaPrep™ LV 32 HSM can be used in a manual mode, where the user performs the pipetting functions. The HSM has an LCD screen that directs the user through the protocol. Features: • Decrease Hands-On Time: Automation reduces operator time spent on instrument setup and takedown by allowing walkaway operation for large numbers of samples at a time. • Remove Protocol Bottlenecks: Heater Shaker Magnet eliminates the need to move samples on the robot deck, reducing instrument failures; precipitation-free chemistry dramatically reduces purification failures. • Achieve Peace of Mind: Automated liquid level sensing for all samples and solutions with operator notification allows recovery of samples in case of error. • Isolate Pure DNA from All Samples: Purification chemistry is equally effective at recovering DNA from pristine as well as challenged (hemolysed or frozen) samples. • Save a Day or Two of Processing: Samples are eluted in buffer, ready for use in downstream assays or archiving, eliminating resuspension of pelleted DNA, which can take 24–48 hours. • Reduce Waste: Chemistry is automatically scaled for each sample, using only the reagent required for optimal purification. Plastic use is also conserved, reducing liquid and solid waste during sample runs. Storage Conditions: Store at 15–30°C. Protocol ReliaPrep™ Large Volume HT gDNA Isolation System Technical Manual Part# TM341 Size Cat.# Price (Fr) 96 × 10ml preps A1751 1997.00 ReliaPrep™ Blood gDNA Miniprep System Product ReliaPrep™ Blood gDNA Miniprep System For Laboratory Use. Description: The ReliaPrep™ Blood gDNA Miniprep System provides a complete, ready-to-use method for purification of gDNA from up to 200μl of blood or body fluid, consistently isolating pure, intact gDNA without the use of alcohol washes or precipitations. Genomic DNA can be prepared from fresh or frozen blood in less than 40 minutes with expected DNA yields of 4–10μg, depending on the white blood cell count of the blood sample. Features: • Easy to Use: Reagents are supplied “ready to go”; no additions required. • Save Time: Eluted DNA obtained in 30 minutes or less. • No Ethanol: Eliminates alcohol inhibition and carryover. • Pure gDNA: Improved A260/A230 ratios vs. the leading competitor. • Peace of Mind: Consistent results from run to run and between users even with hemolyzed samples. • Concentrated DNA: Good recovery and purity in as little as 50μl elution. Storage Conditions: Store at 15–30°C. Protocol ReliaPrep™ Blood gDNA Miniprep System Technical Manual Part# TM330 ReliaPrep™ gDNA Tissue Miniprep System Product ReliaPrep™ gDNA Tissue Miniprep System For Laboratory Use. Description: The ReliaPrep™ gDNA Tissue Miniprep System provides a complete, ready-to-use method for purification of gDNA from up to 25mg of tissue, a buccal (cheek) swab, or a 1cm mouse tail snip, obtaining intact gDNA without the use of ethanol washes or precipitations. Features: • Easy to Use: Reagents are supplied “ready-to-use”; no additions required. • Save Time: Eluted DNA obtained in 30 minutes or less (hands-on time). • No Ethanol: Eliminates alcohol inhibition and carryover. • Pure gDNA: Improved A260/A230 ratios vs. the leading competitor. • Peace of Mind: Consistent results from run to run and between users. • Concentrated DNA: Good recovery and purity in as little as 50μl elution. Storage Conditions: Store at 15–30°C. Protocol ReliaPrep™ gDNA Tissue Miniprep System Technical Manual Part# TM345 Size Cat.# Price (Fr) 100 preps A2051 428.00 250 preps A2052 937.00 Size Cat.# Price (Fr) 100 preps A5081 374.00 250 preps A5082 819.00 110 For complete and up-to-date product information visit: www.promega.com/catalog Pagina 113
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