Cell Signaling ReliaPrep™ FFPE gDNA Miniprep System Product ReliaPrep™ FFPE gDNA Miniprep System For Research Use Only. Not for Use in Diagnostic Procedures. Description: The ReliaPrep™ FFPE gDNA Miniprep System provides a complete, all-inclusive method for purifying quality genomic DNA from formalin-fixed paraffin-embedded tissue without using hazardous solvents or overnight digestion. Genomic DNA can be isolated from FFPE tissue in approximately 2 1/2 hours with minimal hands-on time. Features: • Isolate Quality, Intact gDNA: Optimized lysis and binding conditions reverse modifications introduced by the fixation process, resulting in intact, amplifiable gDNA. • Safely Deparaffinize Your Sample: Deparaffinization step occurs without harsh organic solvents. • Save Time: Purify gDNA from FFPE tissue in less than 2 1/2 hours with minimal hands-on time. No overnight digestion required. • Easy to Use: Minimal preparation time; simply add ethanol and go! Storage Conditions: Store at room temperature. Protocol ReliaPrep™ FFPE gDNA Miniprep System Technical Manual Wizard® Genomic DNA Purification Kit Product Wizard® Genomic DNA Purification Kit Available Separately Cell Lysis Solution (Genomic Purification) Nuclei Lysis Solution Protein Precipitation Solution DNA Rehydration Solution RNase A Solution, 4mg/ml Proteinase K 1 liter A7933 276.00 50 ml A7941 73.00 1 liter A7943 876.00 25 ml A7951 92.00 350 ml A7953 726.00 50 ml A7963 98.00 1 ml A7973 108.00 100 mg V3021 145.00 A7933 For Laboratory Use. A1120, A1125, A7941, A1620, A7943, A7951, A7953, A7963, A7973, V3021 For Research Use Only. Not for Use in Diagnostic Procedures. Size Cat.# Price (Fr) 100 isolations × 300 µl A1120 363.00 500 isolations × 300 µl A1125 1089.00 100 isolations × 10 ml A1620 1929.00 Part# TM352 DNA Yields from Various Starting Materials Using the Wizard® Genomic DNA Purifi cation Kit. Amount of Source Whole Blood Starting Material 300µl 1ml 10ml Tissue Culture Cells Animal Tissue Mouse Liver Mouse Tail Insect Cells Plant Leaf Tissue Bacterial Culture* Yeast* *Overnight culture. 96-well plate, 50µl/well 106–107 cells 11mg 0.5–1cm of tail 5 ⋅ 106 cells 40mg 108–1010 cells 1.9 ⋅ 108 cells Typical DNA Yield 5–15µg 25–50µg 250–500µg 0.2–0.7µg 5–30µg 15–20µg 10–30µg 16µg 7–12µg 5–20µg 4.5–6.5µg 9483LA Size Cat.# Price (Fr) 10 reactions A2351 120.00 100 reactions A2352 560.00 Description: The Wizard® Genomic DNA Purification Kit provides a simple, solution-based method for isolation of DNA from white blood cells, tissue culture cells, animal tissue, plant tissue, yeast and Gram-positive and Gram-negative bacteria. DNA purified with this system is suitable for a variety of applications, including amplification, digestion with restriction endonucleases and membrane hybridizations (e.g., Southern and dot/slot blots). Features: • Improved Productivity: Rapidly isolate genomic DNA from blood, tissue culture, animal and plant cells, bacteria and yeast in approximately 60 minutes. • Scalability: Reagent volumes can be adjusted to correspond to the amount of material to be processed. • Flexibility: Genomic DNA purified from a variety of sample types is suitable for a variety of applications. • Your Choice of Configuration: Learn more about our custom options for this product at: www.promega.com/myway/ Storage Conditions: Store at 22–25°C. Protocol Wizard® Genomic DNA Purification Kit Technical Manual Part# TM050 5 For complete and up-to-date product information visit: www.promega.com/catalog 111 DNA and RNA Purification Pagina 114
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