Science Catalog 2012 Life Cell Signaling Product Wizard® SV Genomic DNA Purification System Size Cat.# Price (Fr) Wizard® SV Genomic DNA Purification System Available Separately Wizard® SV Lysis Buffer Wizard® SV Wash Solution Nuclei Lysis Solution EDTA, 0.5M (pH 8.0), Molecular Biology Grade RNase A Solution, 4mg/ml 50 preps A2360 193.00 250 preps A2361 821.00 50 ml Z3052 72.00 185 ml A1311 136.00 50 ml A7941 73.00 100 ml V4231 33.00 1 ml A7973 108.00 Z3052, A1311 For Laboratory Use. A2360, A2361, A6770, A7941, A6772, V4231, A6774, A7973 For Research Use Only. Not for Use in Diagnostic Procedures. Description: The Wizard® SV Genomic DNA Purification System provides a fast, simple, membrane-based technique for preparing genomic DNA from cultured cells and tissue, including mouse tails. Genomic DNA can be purified from cultured cells in about 20 minutes. Isolation from tissue or mouse tails requires an overnight digestion with Proteinase K (Cat.# V3021). Amplifiable genomic DNA can be isolated from up to 5 × 106 cells, 20mg of tissue or up to 1.2cm of a mouse tail tip without a centrifugation clearing step. The Wizard® SV Genomic DNA Purification System can be used in either a microcentrifuge (spin) or vacuum protocol. Up to 20 samples can be processed at once in the vacuum format with the Vac-Man® Laboratory Vacuum Manifold (Cat.# A7231) and the Vacuum Adapters (Cat.# A1331). Features: • Improved Productivity: Obtain genomic DNA approximately 20 minutes after lysis. • High Yield: Purify 20–30μg of DNA per prep from 1.2cm mouse tail. • Format Choice: Perform purification by either spin or vacuum formats. Storage Conditions: Store at 22–25°C. Protocol Wizard® SV Genomic DNA Purification System Technical Bulletin Part# TB302 Average Yield of Genomic DNA Purifi ed From Various Tissues Using the Wizard® SV and SV 96 Genomic DNA Purifi cation Systems. Sample Type Mouse Tail Clipping Mouse Liver Mouse Heart Mouse Brain CHO Cells NIH/3T3 Cells 293 Cells Starting Amount 20mg 20mg 20mg 20mg 1 ⋅ 106 cells 1 ⋅ 106 cells 1 ⋅ 106 cells Average Yield 20µg 15µg 10µg 6µg 5µg 9µg 8µg 9484LA Wizard® SV 96 Genomic DNA Purification System Product Wizard® SV 96 Genomic DNA Purification System Available Separately Wizard® SV Lysis Buffer Wizard® SV Wash Solution Nuclei Lysis Solution EDTA, 0.5M (pH 8.0), Molecular Biology Grade RNase A Solution, 4mg/ml Wizard® SV 96 Binding Plates Size Cat.# Price (Fr) 1 × 96 preps A2370 430.00 4 × 96 preps A2371 1505.00 50 ml Z3052 72.00 185 ml A1311 136.00 50 ml A7941 73.00 100 ml V4231 33.00 1 ml A7973 108.00 10 pack A2271 1381.00 Z3052, A1311 For Laboratory Use. A2370, A2371, A6780, A7941, A6782, V4231, A6784, A7973, A2271 For Research Use Only. Not for Use in Diagnostic Procedures. Description: The Wizard® SV 96 Genomic DNA Purification System provides a high-throughput, membrane-based technique for consistent preparation of genomic DNA from cultured cells and tissue, including mouse tails. Amplifiable genomic DNA can be isolated from up to 5 × 106 cells, 20mg of tissue or up to 1.2cm of a mouse tail tip without a centrifugation clearing step. With the Wizard® SV Genomic DNA purification system, genomic DNA is purified from cell lysates using 96-well vacuum filtration. Washing the bound DNA requires no disassembly of the manifold, and filtrate waste products are delivered directly to a vacuum trap, eliminating the need to empty waste collection trays. The Wizard® SV Genomic DNA Purification System is designed for use either in a manual format or with Beckman Coulter or PerkinElmer automated instruments. Features: • Improve Productivity: Obtain genomic DNA from mouse tails in 45–60 minutes, genomic DNA from cultured cells in 30 minutes. No centrifugation required. • Achieve High Yield: Purify 20–30μg of DNA per prep from 1.2cm of mouse tail. • Gain Confidence in Applications: Purified DNA ready for amplification. • Automate This Assay: Validated automated methods available at: www.promega.com/automethods/ • Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/myway/ Storage Conditions: Store at 22–25°C. Protocol Part# Wizard® SV 96 Genomic DNA Purification System Technical Bulletin TB303 112 For complete and up-to-date product information visit: www.promega.com/catalog Pagina 115
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