Cell Signaling PureYield™ Plasmid Maxiprep System Product PureYield™ Plasmid Maxiprep System Available Separately Eluator™ Vacuum Elution Device 4 each A1071 161.00 A1071 For Laboratory Use. A2392, A2393, A6730, A6732, A6734 For Research Use Only. Not for Use in Diagnostic Procedures. Description: The PureYield™ Plasmid Maxiprep System isolates transfectionquality plasmid DNA. The system provides a rapid method for purification of up to 1mg of plasmid DNA from a 250ml bacterial culture. Plasmid DNA can be purified rapidly with the vacuum protocol, greatly reducing the time spent on purification compared to silica resin or other membrane-column methods. The PureYield™ Plasmid Maxiprep System incorporates a unique Endotoxin Removal Wash designed to remove substantial amounts of protein, RNA and endotoxin contaminants from purified plasmid DNA. Removal of contaminants improves the robustness of sensitive applications such as eukaryotic transfection, in vitro transcription and coupled in vitro transcription/translation (e.g., TNT® Quick Coupled Transcription/Translation System). Purification is achieved without isopropanol precipitation of purified plasmid DNA. The system has been designed for use with a vacuum source and vacuum manifold (e.g., the Vac-Man® Laboratory Vacuum Manifold). The Eluator™ Vacuum Elution Device is used to elute nucleic acids from PureYield™ Midiprep or Maxiprep columns. It consists of two pieces, a blue base and a clear column assembly. The base interfaces with a Vacuum Manifold that contains Luer-Lok® fittings, such as the Vac-Man® Laboratory Vacuum Manifold (Cat.# A7231), and holds a 1.5ml tube to capture the eluted nucleic acids. The column assembly accepts PureYield™ Midiprep or Maxiprep columns. The Eluator™ Device eliminates the requirement for a centrifuge with a swinging bucket rotor for nucleic acid purification, simplifying and speeding purification protocols. Features: • Improved Productivity: Vacuum protocol simplifies purification of multiple samples at one time. • Confidence in Results: High purity and concentration of plasmid DNA gives proven performance in transfection, in vitro expression and other molecular biology applications. • Ease of Use: Simple protocol eliminates tedious, gravity-drip columns and post-elution alcohol precipitation. • Flexibility: PureYield™ membrane column allows purification of large amounts of plasmid DNA, exceeding the capabilities of other maxiprep systems. Storage Conditions: Store at 22–25°C. Protocol PureYield™ Plasmid Maxiprep System Technical Manual Part# TM280 Wizard® 240 Minutes Size Cat.# Price (Fr) 10 preps A2392 243.00 25 preps A2393 532.00 PureYield™ Done in 60 Minutes 5 Qiagen 240 Minutes Comparison of time required per prep using different systems. PureYield™ system protocols were performed according to the instructions using 250ml overnight cultures of JM109 bacteria transformed with a high-copy-number plasmid. Total times to perform the PureYield™ preps are noted. Other times are estimated based on protocols. 1,000 1,200 200 400 600 800 0 Standard Midi Alternative Midi Standard Maxi Maxiprep 50ml 100ml 150ml 200ml 250ml Bacterial Culture (ml) Plasmid yield from various culture volumes using the PureYield™ Maxiprep and Midiprep Systems. Increasing amounts of JM109 containing the phMGFP plasmid were grown and processed using the PureYield™ Plasmid Systems. Lysate was prepared using the midiprep standard vacuum protocol, the midiprep alternative lysate clearing protocol and the standard maxiprep protocol. The midiprep standard protocol is recommended only for 50ml cultures. For complete and up-to-date product information visit: www.promega.com/catalog 119 DNA and RNA Purification Plasmid Amount (µg) 5816MA 5815MA Pagina 122
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