Science Catalog 2012 Life Cell Signaling Wizard® Plus Minipreps DNA Purification Systems Product Size Cat.# Price (Fr) Wizard® Plus Minipreps DNA Purification System 50 preps A7100 217.00 100 preps A7500 348.00 250 preps A7510 783.00 Available Separately Cell Resuspension Solution (CRA) Cell Lysis Solution (CLA) Neutralization Solution (NSA) Column Wash Solution (CWB) Wizard® Minipreps DNA Purification Resin Wizard® Minicolumns 150 ml A7112 79.00 150 ml A7122 79.00 150 ml A7131 79.00 125 ml A8102 95.00 250 ml A7141 372.00 250 each A7211 372.00 A7112, A7122 For Laboratory Use. A7100, A7500, A7131, A7510, A8102, A7141, A7211 For Research Use Only. Not for Use in Diagnostic Procedures. Description: The resin-based Wizard® Plus Minipreps DNA Purification System provides a simple and reliable method for rapid isolation of plasmid DNA. When using the standard protocol, the entire miniprep process can be completed in 15 minutes or less, with no organic extractions or ethanol precipitations. Minipreps may be processed individually or in multiples with the Vac-Man® (20-sample capacity, Cat.# A7231) or Vac-Man® Jr. (2-sample capacity, Cat.# A7660) Laboratory Vacuum Manifold. DNA is eluted from the Wizard® Minicolumn in Nuclease-Free Water (Cat.# P1193). The purified plasmid can be used directly for automated fluorescent DNA sequencing and restriction digestion without further manipulation and also can be used for in vitro transcription reactions supplemented with a ribonuclease inhibitor, such as Recombinant RNasin® Ribonuclease Inhibitor (Cat.# N2511). The Wizard® Minipreps DNA Purification Resin is used in the isolation and preparation of plasmid DNA in conjunction with the Wizard® Plus Minipreps DNA Purification Systems. The resin is available with the systems and as a standalone product. Features: • High Performance: DNA is suitable for most molecular biology applications, including fluorescent sequencing. • Confidence in Results: Purified DNA meets a target of >98% accuracy over 500 bases using pGEM®-3Zf(+) Vector in BigDye® terminator sequencing. • Fast: Entire procedure may be completed in 15 minutes or less. • Convenient: No phenol extractions or ethanol precipitations required. Storage Conditions: Store at 22–25°C. Protocol Part# Wizard® Plus Minipreps DNA Purification System Technical Bulletin TB117 Wizard® Plus Midipreps DNA Purification System Product Cell Resuspension Solution (CRA) Cell Lysis Solution (CLA) Neutralization Solution (NSA) Column Wash Solution (CWB) Wizard® Midipreps DNA Purification Resin Wizard® Midicolumns Size Cat.# Price (Fr) Wizard® Plus Midipreps DNA Purification System 25 preps A7640 314.00 Available Separately 150 ml A7112 79.00 150 ml A7122 79.00 150 ml A7131 79.00 125 ml A8102 95.00 1,000 ml A7701 850.00 100 each A7651 277.00 A7112, A7122 For Laboratory Use. A7640, A7131, A8102, A7701, A7651 For Research Use Only. Not for Use in Diagnostic Procedures. Description: The resin-based Wizard® Plus Midipreps DNA Purification System provides a simple and reliable method for rapid isolation of plasmid DNA. When using the standard protocol, the entire midiprep process can be completed in 90 minutes or less, yielding up to 200μg of high-quality DNA with no organic extractions or ethanol precipitations. Multiple midipreps can be easily processed at one time with the Vac-Man® (20-sample capacity, Cat.# A7231) or Vac-Man® Jr. (2-sample capacity, Cat.# A7660) Laboratory Vacuum Manifold. DNA is eluted from the Wizard® Midicolumn in Nuclease-Free Water (Cat.# P1193). The purified plasmid can be used directly for automated fluorescent DNA sequencing or restriction digestion without further manipulation and also can be used for in vitro transcription reactions supplemented with a ribonuclease inhibitor such as Recombinant RNasin® Ribonuclease Inhibitor (Cat.# N2511). The system includes sufficient reagents for 25 DNA isolations from 10–100ml of liquid culture. Features: • Fast: Rapid batch column method used for DNA isolation. • Safe: Eliminates the need for cesium chloride:ethidium bromide gradient centrifugation and does not require organic extractions. • Reliable: Yields plasmid DNA of comparable quantity and quality to cesium chloride:ethidium bromide gradient techniques that are much more timeand labor-intensive. • High Performance: DNA is suitable for restriction enzyme digestions, automated fluorescent DNA sequencing, transformation and subcloning. • Confidence in Results: Purified DNA meets a target of >98% accuracy over 500 bases using pGEM®-3Zf(+) Vector in BigDye® terminator sequencing. Storage Conditions: Store at 22–25°C. Protocol Part# Wizard® Plus Midipreps DNA Purification System Technical Bulletin TB173 120 For complete and up-to-date product information visit: www.promega.com/catalog Pagina 123
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