Cell Signaling Wizard® Plus Maxipreps DNA Purification System Product Cell Resuspension Solution (CRA) Cell Lysis Solution (CLA) Neutralization Solution (NSA) Column Wash Solution (CWB) Wizard® Maxipreps DNA Purification Resin Wizard® Maxi/Megapreps Filtering System Size Cat.# Price (Fr) Wizard® Plus Maxipreps DNA Purification System 10 preps A7270 252.00 Available Separately 150 ml A7112 79.00 150 ml A7122 79.00 150 ml A7131 79.00 125 ml A8102 95.00 500 ml A7401 642.00 50 each A7421 429.00 A7112, A7122 For Laboratory Use. A7270, A7131, A8102, A7401, A7421 For Research Use Only. Not for Use in Diagnostic Procedures. Description: The Wizard® Plus Maxipreps DNA Purification System provides a simple and rapid resin-based batch column method for purification of plasmid DNA that eliminates the need for cesium chloride:ethidium bromide gradient centrifugation. Use of this system requires only a centrifuge, a vacuum source and a vacuum manifold. This system typically yields 300μg–1mg of high-copynumber plasmid DNA (200–20,000bp) from a 100–500ml culture in less than 3 hours. The purified DNA is eluted in Nuclease-Free Water (Cat.# P1193) and can be used directly for DNA sequencing and restriction digestion without further manipulation. The DNA also can be used for in vitro transcription reactions supplemented with a ribonuclease inhibitor such as Recombinant RNasin® Ribonuclease Inhibitor (Cat.# N2511). Features: • Flexible: DNA is suitable for restriction enzyme digestions, automated fluorescent DNA sequencing, transformation and subcloning. • High Quality: Yields plasmid DNA of comparable quantity and quality to cesium chloride:ethidium bromide gradient techniques that are much more time- and labor-intensive. • Fast: Rapid batch binding and column washing method used for DNA isolation. • Safe: Eliminates the need for cesium chloride:ethidium bromide gradient centrifugation and does not require organic extractions. Storage Conditions: Store at 22–25°C. Protocol Part# Wizard® Plus Maxipreps DNA Purification System Technical Bulletin TB139 Wizard® Plus Megapreps DNA Purification System Product Cell Resuspension Solution (CRA) Cell Lysis Solution (CLA) Neutralization Solution (NSA) Column Wash Solution (CWB) Wizard® Megapreps DNA Purification Resin Wizard® Maxi/Megapreps Filtering System Size Cat.# Price (Fr) Wizard® Plus Megapreps DNA Purification System 5 preps A7300 285.00 Available Separately 150 ml A7112 79.00 150 ml A7122 79.00 150 ml A7131 79.00 125 ml A8102 95.00 1,000 ml A7361 1139.00 50 each A7421 429.00 A7112, A7122 For Laboratory Use. A7300, A7131, A8102, A7361, A7421 For Research Use Only. Not for Use in Diagnostic Procedures. Description: Wizard® Plus Megapreps DNA Purification System provides a simple and rapid method for large-scale purifications of plasmid DNA that eliminates the need for cesium chloride:ethidium bromide gradient centrifugation. Use of this system requires only a centrifuge, a vacuum source and a vacuum manifold. The system yields greater than one milligram of highcopy-number plasmid DNA (200–20,000bp) from a 1,000ml culture in less than three hours. The purified DNA is eluted in Nuclease-Free Water (Cat.# P1193) or TE buffer and can be used directly for DNA sequencing and restriction digestion without further manipulation. The DNA also can be used for in vitro transcription reactions supplemented with a ribonuclease inhibitor such as Recombinant RNasin® Ribonuclease Inhibitor (Cat.# N2511). Features: • Fast: Rapid batch binding and column washing method used for DNA isolation • Safe: Eliminates the need for cesium chloride:ethidium bromide gradient centrifugation and does not require organic extractions. • Reliable: Yields plasmid DNA of comparable quantity and quality to cesium chloride:ethidium bromide gradient techniques that are much more timeand labor-intensive. • Yield: Each megaprep produces >1mg of DNA from 1,000ml of bacterial culture when using a high-copy-number plasmid. • Quality: DNA is suitable for restriction enzyme digestions, automated fluorescent DNA sequencing, transformation and subcloning. Storage Conditions: Store at 22–25°C. Protocol Part# Wizard® Plus Megapreps DNA Purification System Technical Bulletin TB140 5 For complete and up-to-date product information visit: www.promega.com/catalog 121 DNA and RNA Purification Pagina 124
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