Cell Signaling Sephacryl® S-400 Product Sephacryl® S-400 For Laboratory Use. Size Cat.# Price (Fr) 10 ml V3181 82.00 Description: Sephacryl® S-400 is a chromatography matrix used for rapid gel filtration. This matrix is useful in experiments involving the incorporation of synthetic linkers and adaptors. After linker ligation and digestion with the appropriate enzyme, unincorporated linkers and linker fragments may be rapidly removed from the DNA sample using spin columns containing Sephacryl® S-400. Such columns may be used to separate small DNA fragments (≤271bp) from longer DNA molecules. Composition: Suspension in 10mM Tris-HCl (pH 8.0), 100mM NaCl and 1mM EDTA. Features: • Quality Tested: Each lot is tested and certified to be free of DNase and RNase activity. Storage Conditions: Store at 4°C. Sodium Chloride, Molecular Biology Grade Product Sodium Chloride, Molecular Biology Grade For Research Use Only. Not for Use in Diagnostic Procedures. Description: Sodium Chloride, Molecular Biology Grade, is commonly used in many molecular biology and forensic applications. Formula Weight: 58.45. Properties: • Purity: ≥99.5%. • Iron: ≤2ppm. • Lead: ≤5ppm. • pH at 25°C of 1M: 5.0–8.0. • A260 at 1M: ≤0.02. • A280 at 1M: ≤0.01. • Conductivity at 25°C (0.05M): 5,000–7,000μSm. Features: • Quality Tested: Each lot of Sodium Chloride is tested and certified to be free of DNase, RNase and protease activity. Storage Conditions: Store at 22–25°C. Size Cat.# Price (Fr) 500 g H5271 49.00 1 kg H5273 89.00 Sodium Dodecyl Sulphate, Molecular Biology Grade (SDS) Product Sodium Dodecyl Sulphate, Molecular Biology Grade (SDS) For Research Use Only. Not for Use in Diagnostic Procedures. Description: Sodium Dodecyl Sulphate, Molecular Biology Grade (SDS), is a detergent that is known to denature proteins. It is used in denaturing polyacrylamide gel electrophoresis for the determination of protein molecular weight. It is also used in nucleic acid extraction procedures for the disruption of cell walls and dissociation of nucleic acid:protein complexes. Formula Weight: 288.38. Properties: • Purity: ≥99.5%. • pH at 25°C (3% w/v): 6.0–7.5. • A230 at 3%: ≤0.40. • A260 at 3%: ≤0.30. • A280 at 3%: ≤0.05. • A405 at 3%: ≤0.01. Features: • Quality Tested: Each lot is tested and certified to be free of DNase and RNase activity. Storage Conditions: Store at 22–25°C. SSC Buffer, 20X, Molecular Grade Product SSC Buffer, 20X, Molecular Grade For Research Use Only. Not for Use in Diagnostic Procedures. Description: SSC Buffer, 20X, Molecular Grade (pH 7.0), is commonly used in nucleic acid hybridization techniques at concentrations from 0.1X to 20X, depending on the application. Form: Clear, colorless liquid. Composition: 3M NaCl, 0.3M sodium citrate (for 20X concentration). Properties: • pH at 25°C (20X): 6.9–7.1. • Lead: ≤10ppm. • Conductivity at 25°C (2X): 24.4–32.4mmhos. Features: • Quality Tested: Each lot of SSC Buffer is tested and certified to be free of DNase, RNase and protease activity. Storage Conditions: Store at 22–25°C. Size Cat.# Price (Fr) 1,000 ml V4261 66.00 Size Cat.# Price (Fr) 100 g H5113 57.00 500 g H5114 243.00 1 kg H5115 438.00 1 For complete and up-to-date product information visit: www.promega.com/catalog 9 Biochemicals and Labware Pagina 12
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