Science Catalog 2012 Life Cell Signaling Streptavidin Product Streptavidin For Laboratory Use. Description: Promega Streptavidin is purified by affinity chromatography and is of the highest quality available. Storage Conditions: Store at –20°C. Streptavidin Alkaline Phosphatase Product Streptavidin Alkaline Phosphatase For Laboratory Use. Size Cat.# Price (Fr) 0.5 ml V5591 213.00 Description: Streptavidin Alkaline Phosphatase is used for the detection of biotinylated molecules. Composition: Conjugated Streptavidin Alkaline Phosphatase in PBS, 1mg/ml BSA, 1mM MgCl2, 0.1mM ZnCl2 and 0.02% sodium azide. Features: • Quality Tested: Streptavidin Alkaline Phosphatase is quality tested to ensure optimal performance for the detection of biotinylated molecules. Storage Conditions: Store at 4°C. Do not freeze! TAE Buffer, Molecular Biology Grade (Trisacetate-EDTA) Product Size Conc. Cat.# Price (Fr) TAE Buffer, 10X, Molecular Biology Grade 1,000 ml 10 X V4271 66.00 TAE Buffer, 40X, Molecular Biology Grade 1,000 ml 40 X V4281 132.00 For Research Use Only. Not for Use in Diagnostic Procedures. Description: TAE Buffer is the most commonly used buffer for agarose DNA electrophoresis. A 1X solution is obtained by adding 1 part of the concentrated TAE to 9 or 39 parts of deionized water. Form: Clear, colorless liquid. Properties: • Composition (10X): 400mM Tris-acetate, 10mM EDTA. • Composition (40X): 1.6M Tris-acetate, 40mM EDTA. • pH at 25°C: 8.2–8.4. • Lead: ≤10ppm. Features: • Quality Tested: Each lot of TAE Buffer is tested and certified to be free of DNase, RNase and protease activity. Storage Conditions: Store at 22–25°C. Size Cat.# Price (Fr) 1 mg Z7041 117.00 TBE Buffer, 10X, Molecular Biology Grade Product TBE Buffer, 10X, Molecular Biology Grade For Research Use Only. Not for Use in Diagnostic Procedures. Description: TBE Buffer, 10X (pH 8.3), is used for polyacrylamide and agarose gel electrophoresis. This product has been optimized for use in DNA applications. Form: Clear, colorless liquid. Composition: 890mM Tris-borate, 890mM boric acid, 20mM EDTA. Properties: • pH at 25°C (1X): 8.2–8.4. Features: • Quality Tested for DNase Activity: Each lot of TBE Buffer is tested and demonstrates ≤1% release. • Quality Tested for RNase Activity: Each lot of TBE Buffer is tested and demonstrates ≤1% release. Storage Conditions: Store at 22–25°C. TE Buffer, 1X, Molecular Biology Grade Product TE Buffer, 1X, Molecular Biology Grade For Research Use Only. Not for Use in Diagnostic Procedures. Description: TE Buffer, 1X, Molecular Grade (pH 8.0), is a buffer composed of 10mM Tris-HCl containing 1mM EDTA•Na2. Properties: • pH at 25°C: 7.9–8.1. • A280: ≤0.05. Features: • Quality Tested: Each lot of TE Buffer is tested and certified to be free of DNase, RNase and protease activity. Storage Conditions: Store at 22–25°C. TMB Stabilized Substrate for Horseradish Peroxidase Product TMB Stabilized Substrate for Horseradish Peroxidase For Research Use Only. Not for Use in Diagnostic Procedures. Description: TMB Stabilized Substrate is a stable, ready-to-use TMB (3,3´, 5,5´-tetramethylbenzidine) color development substrate for localization of horseradish peroxidase-conjugated antibodies on dot blots and Western blots. It is easier to use than 4-chloro-1-naphthol (CN), which must be prepared immediately before use. TMB Stabilized Substrate comes premixed and fully diluted in a proprietary buffer containing less than 0.5% organic solvent. Features: • Convenient: Premixed, ready-to-use; in proprietary buffer containing less than 0.5% organic solvents. • Stable: Stable at room temperature for 12 months. • Sensitive: At least threefold more sensitive than 4-chloro-1-naphthol (CN); as little as 412pg of β-galactosidase detected on TMB blot as compared to 1.12ng on CN blot when detected with a β-galactosidase-specific antibody and HRP-conjugated secondary antibody. • Long-Lasting Color: Color is much more stable than 4-chloro-1-naphthol and photographs more easily. Storage Conditions: Store at 22–25°C. 10 For complete and up-to-date product information visit: www.promega.com/catalog Size Cat.# Price (Fr) 200 ml W4121 221.00 Size Cat.# Price (Fr) 100 ml V6231 52.00 500 ml V6232 221.00 Size Cat.# Price (Fr) 1,000 ml V4251 66.00 Pagina 13
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