Cell Signaling PowerPlex® 18D System Product PowerPlex® 18D System Available Separately CC5 Internal Lane Standard 500 Water, Amplification Grade 2800M Control DNA Size Cat.# Price (Fr) 200 reactions DC1802 6799.00 800 reactions DC1808 23797.00 Size Conc. 300 µl 6,250 µl Cat. # Price (Fr) DG1521 495.00 DW0991 47.00 25 µl 10 ng/µl DD7101 160.00 DG1521, DW0991 For Laboratory Use. DC1802, DC1808, DD7101 Not For Medical Diagnostic Use. Description: The PowerPlex® 18D System is a multiplex STR system for use in database and paternity testing. This system is optimized for direct amplification of sample on FTA® cards. This five-color multiplex allows co-amplification of the 13 CODIS loci (D18S51, D21S11, TH01, D3S1358, FGA, TPOX, D8S1179, vWA, CSF1PO, D16S539, D7S820, D13S317, D5S818) plus Amelogenin, Penta E, Penta D, D2S1338 and D19S433. All eighteen loci are amplified simultaneously in a single tube and analyzed in a single injection. The PowerPlex® 18D System is compatible with ABI PRISM® 3100 and 3100-Avant Genetic Analyzers and Applied Biosystems 3130, 3130xl, 3500 and 3500xL Genetic Analyzers. The PowerPlex 18D System was given NDIS approval in July 2011 for NDIS CODIS databasing. Configuration of the PowerPlex® 18D System. The PowerPlex® 18D System contains all 13 CODIS loci: D3S1358, TH01, D21S11, D18S51, D5S818, D13S317, D7S820, D16S539, CSF1PO, vWA, D8S1179, TPOX and FGA, plus Amelogenin, Penta E, Penta D, D19S433 and D2S1338. Features: • Eliminate DNA Extraction: Simplify and shorten sample processing with direct amplification from FTA® cards. • Reduce PCR Time: Amplify in less than 1.5 hours using rapid cycling technology. • Upload More Markers: Type D2S1338, D19S433, Penta D, Penta E, Amelogenin and the 13 CODIS loci with one kit. • Automatically Assign Genotypes: Panels and bins text files are required for automatic assignment of genotypes using the GeneMapper® ID and ID-X software and are available for download. Storage Conditions: Store kit at –20°C. Upon receipt, remove 2800M Control DNA and store at 4°C. Protocol PowerPlex® 18D System Technical Manual Part# TMD031 7 A. B. C. D. E. Amplification of sample using the PowerPlex® 18D System. Two 1.2mm punches were taken from a buccal sample transferred to an FTA® card and amplified for 27 cycles using the PowerPlex® 18D System. Amplification products were mixed with CC5 Internal Lane Standard 500 and analyzed with an Applied Biosystems 3130xl Genetic Analyzer using a 3kV, 5-second injection. Results were analyzed using GeneMapper® ID software, version 3.2. For complete and up-to-date product information visit: www.promega.com/catalog 153 Genetic Identity 9654TA 9682TA Pagina 156
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