Science Catalog 2012 Life Cell Signaling PowerPlex® 16 HS System Product PowerPlex® 16 HS System Available Separately Internal Lane Standard 600 9947A DNA Water, Amplification Grade Size Cat.# Price (Fr) 100 reactions DC2101 3253.00 400 reactions DC2100 11386.00 Size Conc. 150 µl Cat. # Price (Fr) DG1071 472.00 250 ng 10 ng/µl DD1001 Pls. Enq. 6,250 µl DW0991 47.00 DG1071, DW0991 For Laboratory Use. DC2101, DC2100, DD1001 Not For Medical Diagnostic Use. Description: The PowerPlex® 16 HS System is a multiplex STR system for use in DNA typing. This system co-amplifies the loci D18S51, D21S11, TH01, D3S1358, Penta E (labeled with fluorescein); FGA, TPOX, D8S1179, vWA and Amelogenin (labeled with TMR); and CSF1PO, D16S539, D7S820, D13S317, D5S818 and Penta D (labeled with JOE). This multiplex includes all 13 CODIS STR markers, Amelogenin for gender determination and two low-stutter, highly discriminating pentanucleotide STR markers. All sixteen loci are amplified simultaneously in a single tube and analyzed in a single injection. The PowerPlex® 16 HS System is compatible with ABI PRISM® 310, 3100 and 3100-Avant Genetic Analyzers and Applied Biosystems 3130, 3130xl, 3500 and 3500xL Genetic Analyzers. Features: • Robustness: The PowerPlex® 16 HS System is more tolerant of PCR inhibitors than competing STR systems and the previous version of PowerPlex® 16. Generate profiles with samples that previously failed to amplify. Avoid costly and time-consuming sample cleanup. • Sensitivity: Each lot is quality tested to produce full profiles from 100pg of DNA. Gain confidence in analysis of limited samples. • High Discrimination: The loci included in the PowerPlex® 16 HS System are more discriminating than competitive systems and are ideal for resolving partial matches or challenging familial cases. • Proven Design: Primer sequences, dyes and ladders are all unchanged from PowerPlex® 16. Expect concordance with existing databases. • Complete System: The PowerPlex® 16 HS System includes size standard, amplification-grade water and Taq DNA polymerase in the master mix. Simple to order, easy to use. • Automatic Assignment of Genotypes: Panels and bins text files are required for automatic assignment of genotypes using the GeneMapper® ID and ID-X software and are available for download. Storage Conditions: Store at –20°C. Protocol PowerPlex® 16 HS System Technical Manual Part# TMD022 PowerPlex® 16 System Product PowerPlex® 16 System Available Separately Internal Lane Standard 600 9947A DNA Size Cat.# Price (Fr) 100 reactions DC6531 3253.00 400 reactions DC6530 11386.00 Size Conc. 150 µl DG1071 For Laboratory Use. DC6531, DC6530, DD1001 Not For Medical Diagnostic Use. Description: The PowerPlex® 16 System is a multiplex STR system for use in DNA typing, including paternity testing, forensic DNA analysis, human identity testing and tissue culture strain identification. The system allows co-amplification and three-color detection of sixteen loci (fifteen STR loci and Amelogenin): Penta E, D18S51, D21S11, TH01, D3S1358, FGA, TPOX, D8S1179, vWA, Amelogenin, Penta D, CSF1PO, D16S539, D7S820, D13S317 and D5S818. One primer for each of the Penta E, D18S51, D21S11, TH01 and D3S1358 loci is labeled with fluorescein (FL); one primer for each of the FGA, TPOX, D8S1179, vWA and Amelogenin loci is labeled with carboxy-tetramethylrhodamine (TMR); and one primer for each of the Penta D, CSF1PO, D16S539, D7S820, D13S317 and D5S818 loci is labeled with 6-carboxy-4´,5´-dichloro-2´,7´dimethoxy-fluorescein (JOE). All sixteen loci are amplified simultaneously in a single tube and analyzed in a single injection or gel lane. The PowerPlex® 16 System is compatible with the ABI PRISM® 310, 3100 and 3100-Avant Genetic Analyzers, Applied Biosystems 3130 and 3130xl Genetic Analyzers and ABI PRISM® 377 DNA Sequencer. Features: • Single Amplification: Amplify all 13 CODIS STR loci in one reaction. • Maximum Sensitivity: Analyze minute quantities of DNA. The system is optimized for use with 1ng of DNA; however, each lot is performance tested to provide reproducible results with 0.5ng of DNA. Additional studies show interpretable results can be obtained with <0.5ng of DNA. • Internal Lane Standard 600: This marker offers the greatest precision available for DNA typing. It is used in each gel lane or capillary injection to increase the precision of analysis. • Allelic Ladders: Allelic ladders for all 16 loci are provided to simplify interpretation. • Automatic Assignment of Genotypes: Panels and bins text files are required for use with GeneMapper® ID software and are available for download. The PowerTyper™ 16 Macro (available separately) facilitates data analysis, allowing automatic assignment of genotypes using the Genotyper® software. The PowerTyper™ Macros can be downloaded or ordered on CD-ROM. • Validation Studies: The PowerPlex® 16 System has undergone extensive validation efforts including internal validation, CODIS database validation and forensic casework validation. Storage Conditions: Store at –20°C. The fluorescent primer pairs and allelic ladders are light-sensitive; therefore, minimize light exposure. Protocol PowerPlex® 16 System Technical Manual PowerPlex® 16 BIO System Part# TMD012 Cat. # Price (Fr) DG1071 472.00 250 ng 10 ng/µl DD1001 Pls. Enq. 154 For complete and up-to-date product information visit: www.promega.com/catalog Pagina 157
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