Cell Signaling PowerPlex® CS7 System, Custom Product PowerPlex® CS7 System, Custom Available Separately Internal Lane Standard 600 9947A DNA Water, Amplification Grade Size Conc. 150 µl Size Cat.# Price (Fr) 100 reactions X6613 1700.00 Cat. # Price (Fr) DG1071 472.00 250 ng 10 ng/µl DD1001 Pls. Enq. 6,250 µl DG1071, DW0991 For Laboratory Use. X6613, DD1001 Not For Medical Diagnostic Use. Description: The PowerPlex® CS7 System, Custom, is a multiplex STR assay for relationship testing and human identification. The PowerPlex® CS7 System, Custom, allows co-amplification and three-color detection of seven STR loci, including LPL, F13B, FESFPS, F13A01, Penta D, Penta C and Penta E. All seven loci are amplified simultaneously in a single tube and analyzed in a single injection. The PowerPlex® CS7 System, Custom, contains two loci, Penta D and Penta E, that overlap with the loci included in the PowerPlex® 16 and PowerPlex® 16 HS Systems. This feature allows the PowerPlex® CS7 System, Custom, to be used as a confirmatory kit in paternity applications using the five unshared STR loci to supplement the genotype and increase the available information. The PowerPlex® CS7 System, Custom, is compatible with the ABI PRISM® 3100 and 3100-Avant Genetic Analyzers and Applied Biosystems 3130 and 3130xl Genetic Analyzers. The PowerPlex® CS7 System, Custom, provides all materials necessary to amplify STR regions of purified genomic DNA. Features: • More Loci: Supplement current testing with LPL, F13B, FESFPS, F13A01 and Penta C for greater discrimination. • Confirm Results: Overlap of Penta D and Penta E in the PowerPlex® CS7 and 16 HS Systems allow detection of sample mixup when used together. • Complete: Hot-start Taq DNA Polymerase is provided in the master mix, and size standard is included. Storage Conditions: Store at –20°C. Protocol PowerPlex® CS7 System Custom Technical Manual Part# TMD030 DW0991 47.00 PowerPlex® S5 System Product PowerPlex® S5 System Available Separately Internal Lane Standard 600 9947A DNA Water, Amplification Grade Size Cat.# Price (Fr) 100 reactions DC6951 1555.00 400 reactions DC6950 5443.00 Size Conc. 150 µl Cat. # Price (Fr) DG1071 472.00 250 ng 10 ng/µl DD1001 Pls. Enq. 6,250 µl DW0991 47.00 DG1071, DW0991 For Laboratory Use. DC6951, DC6950, DD1001 Not For Medical Diagnostic Use. Description: The PowerPlex® S5 System is a mini-STR kit that allows co-amplification and detection of four STR markers (D18S51, D8S1179, TH01 and FGA) plus Amelogenin. One primer specific for each of the Amelogenin, D18S51 and D8S1179 loci is labeled with fluorescein (FL), and one primer specific for each of the TH01 and FGA loci is labeled with 6-carboxy-4´,5´dichloro-2´,7´-dimethoxy-fluorescein (JOE). All five loci are amplified simultaneously in a single tube and analyzed in a single injection. The four STR loci are included in the CODIS and European databases. The amplicons for all loci are smaller than 260bp. The PowerPlex® S5 System was the first Promega STR kit to include hot-start Taq DNA polymerase, which is included in the PowerPlex® S5 5X Master Mix. The PowerPlex® S5 System is primarily a screening tool but also can be used as a mini-STR casework kit. Features: • Sensitive: Generate full DNA profiles with as little as 50pg of DNA. • Easy to Use: The PowerPlex® S5 System comes complete with premixed primer pairs, a master mix with Taq DNA polymerase and internal lane standard. The simplified thermal cycling protocol requires no ramping, and the system is compatible with a number of instrument platforms, including ABI PRISM® 310, 3100 and 3100-Avant and Applied Biosystems 3130 and 3130xl Genetic Analyzers. • Robust: The PowerPlex® S5 System is more tolerant of DNA degradation and less sensitive to inhibitors. Full DNA profiles can be achieved in the presence of 130μM hematin, 200ng tannic acid or 150ng humic acid. • Automatic Assignment of Genotypes Available: Panels and bins text files are required for use with GeneMapper® ID software and are available for download. The PowerTyper™ S5 Macro (available separately) facilitates data analysis, allowing automatic assignment of genotypes using the Genotyper® software. The PowerTyper™ Macros can be downloaded or ordered on CD-ROM. Storage Conditions: Store at –20°C. Protocol PowerPlex® S5 System Technical Manual Part# TMD021 7 For complete and up-to-date product information visit: www.promega.com/catalog 155 Genetic Identity Pagina 158
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