Science Catalog 2012 Life Cell Signaling PowerPlex® Y System Product PowerPlex® Y System Available Separately Internal Lane Standard 600 9948 Male DNA 9947A DNA Size Cat.# Price (Fr) 50 reactions DC6761 2455.00 200 reactions DC6760 8593.00 Size Conc. 150 µl Cat. # Price (Fr) DG1071 472.00 250 ng 10 ng/µl DD2061 199.00 250 ng 10 ng/µl DD1001 Pls. Enq. DG1071 For Laboratory Use. DC6761, DC6760, DD2061, DD1001 Not For Medical Diagnostic Use. Description: The PowerPlex® Y System allows co-amplification and threecolor detection of twelve loci. The system contains primers for the loci DYS19, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438 and DYS439. One primer specific for DYS389I/II, DYS391 and DYS439 is labeled with fluorescein (FL). One primer specific for each of the DYS385a/b, DYS390 and DYS393 loci is labeled with carboxy-tetramethylrhodamine (TMR). One primer specific for each of the DYS19, DYS392, DYS437 and DYS438 loci is labeled with 6-carboxy-4´,5´-dichloro-2´,7´-dimethoxy-fluorescein (JOE). All twelve loci are amplified simultaneously in a single tube and analyzed in a single injection or gel lane. Fragment sizing is provided by an internal size standard (Internal Lane Standard 600) labeled with carboxy-X-rhodamine (CXR). Color deconvolution can be performed using matrix standards available from Promega. The PowerPlex® Y System is compatible with the ABI PRISM® 310, 3100 and 3100-Avant and Applied Biosystems 3130 and 3130xl Genetic Analyzers and ABI PRISM® 377 DNA Sequencer. Features: • Amplification of 12 Y-STR Loci in One Reaction: Amplify the nine loci defined as the European minimal haplotype and endorsed by ISFG (International Society of Forensic Genetics)* plus the two loci added to this panel by SWGDAM (Scientific Working Group on DNA Analysis Methods). Note: The data in the PowerPlex® Y Haplotype Database have been added to the US Y-STR Database, a searchable listing of 11- to 17-locus Y-STR haplotypes. For Y-STR haplotype searches, visit the US Y-STR Database at: www.usystrdatabase.org or Y-Chromosome Haplotype Reference Database at: www.yhrd.org *ISFG-endorsed loci are DYS19, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392, DYS393. SWGDAM-endorsed loci are DYS19, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS438 and DYS439. • Maximum Sensitivity: The system is optimized for use with 0.5–1ng of template DNA. However, each lot is performance tested to provide reproducible results with 0.25ng of DNA. Additional studies show interpretable results can be obtained with <0.25ng of DNA. • Superior Specificity—You Amplify Only Male DNA: Each lot is performance tested to ensure no amplification with up to 100ng of female DNA. Additional studies show no amplification with >100ng of female DNA. This makes the PowerPlex® Y System a great choice to analyze “heavy” mixture samples. • Short PCR Products: Amplification products are all less than 335 bases, which increases the likelihood of obtaining a full profile from degraded samples. • No Locus Overlap: Primers were designed so that alleles for each locus do not overlap with a neighboring locus, reducing the likelihood of misinterpretation. • Typing of Samples with Greater Confidence: Allelic ladders for all 12 loci are provided to simplify interpretation through the use of the PowerTyper™ Macro. The wide span of alleles included in the ladder allows typing of more rare alleles. • Automatic Assignment of Genotypes: The PowerTyper™ Y Macro (available separately) automatically labels fragments from Genotyper® data. The PowerTyper™ Macros can be downloaded or ordered on CD-ROM. Panels and bins text files for use with GeneMapper® ID are available for download. Storage Conditions: Store at –20°C. The fluorescent primer pairs and allelic ladders are light-sensitive; therefore, minimize light exposure. Protocol PowerPlex® Y System Technical Manual A. Part# TMD018 B. C. The PowerPlex® Y System. A single-source template DNA (0.5ng) was amplified using the PowerPlex® Y 10X Primer Pair Mix. The amplification products were analyzed with an Applied Biosystems 3130 Genetic Analyzer using a 3kV, 5-second injection. The results were analyzed using GeneMapper® ID software, version 3.2. Panel A. An electropherogram showing the peaks of the fluorescein-labeled loci: DYS389I/II, DYS391 and DYS439. Panel B. An electropherogram showing the peaks of the JOE-labeled loci: DYS19, DYS392, DYS437 and DYS438. Panel C. An electropherogram showing the peaks of the TMR-labeled loci: DYS385a/b, DYS390 and DYS393. 156 For complete and up-to-date product information visit: www.promega.com/catalog 6043TA Pagina 159

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