Science Catalog 2012 Life Cell Signaling Vitronectin, Human Product Vitronectin, Human For Research Use Only. Not for Use in Diagnostic Procedures. Description: Human Vitronectin is purified from plasma. Vitronectin belongs to the group of structurally and functionally homologous adhesive proteins (fibrinogen, fibronectin, Von Willebrand factor) that interact with platelets and the vessel wall in the early stages of blood clotting. When coated on surfaces, very low concentrations of Vitronectin promote endothelial cell attachment and induce spreading and migration of cells in a time- and concentrationdependent fashion. Activity: When coated onto tissue culture plastic, Vitronectin promotes onehalf maximal attachment of BALB/3T3 fibroblasts in serum-free medium below 0.1μg/cm2. Maximal attachment occurs at approximately 0.2μg/cm2. Storage Conditions: Store at –70°C. Protocol Vitronectin, Human Protocol Western Blue® Stabilized Substrate for Alkaline Phosphatase Product Western Blue® Stabilized Substrate for Alkaline Phosphatase For Research Use Only. Not for Use in Diagnostic Procedures. Description: Western Blue® Stabilized Substrate for Alkaline Phosphatase is a stable, ready-to-use substrate for Western blots and immunoscreening. Western Blue® Substrate should be used directly and without dilution. It is a mixture of 5-bromo-4-chloro-3-indolyl-phosphate (BCIP) and nitro blue tetrazolium (NBT) in a proprietary stabilizing buffer. This liquid substrate deposits a permanent dark purple stain on membrane sites bearing alkaline phosphatase. Western Blue® Substrate is as sensitive as BCIP/NBT reagents. Features: • Convenient: Use directly without diluting or mixing. • Sensitive: Substrate is as sensitive as other BCIP/NBT reagents. • Stable: Stable for one year at room temperature. Storage Conditions: Store at 22–25°C. X-Gal (5-bromo-4-chloro-3-indolyl-β-dgalactopyranoside) Product X-Gal For Laboratory Use. Description: X-Gal, in conjunction with IPTG, is used to detect β-galactosidase activity to differentiate recombinants from nonrecombinants in cloning experiments using vectors containing the lacZ or lacZ α-peptide gene. Features: • Concentration: 50mg/ml in dimethylformamide, 2.0ml/vial. • Quality Tested: X-Gal is tested for use with the pGEM®-Z Vectors in a chromogenicity assay. Storage Conditions: Store at 4°C or –20°C. Size Conc. Cat.# Price (Fr) 100mg/2 ml 50 mg/ml V3941 95.00 Size Cat.# Price (Fr) 100 ml S3841 92.00 For Laboratory Use. Description: Herring Sperm DNA is tested and certified to be free of any DNase or RNase activity. It is useful as a blocking agent in nucleic acid hybridization experiments. Features: • Quality Tested: Certified to be free of any DNase or RNase activity. • Multiple Applications: Use as a blocking agent in hybridizations or as carrier DNA. • Ready to Use: Provided as a 10mg/ml solution. • Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/myway/ Storage Conditions: Store at –20°C. Note: Product may be viscous at 4°C. Prior to use, ensure product is at room temperature (it may be briefly warmed at 37°C) and mixed thoroughly to ensure homogeneity. Lambda DNA Product Lambda DNA For Laboratory Use. Description: λ DNA cl857 Sam7 is isolated from infected E. coli strain W3350. Restriction enzyme-digested λ DNA (48,502bp) may be used as a molecular weight size marker in gel analysis of nucleic acids. λ DNA is also a commonly used substrate in restriction enzyme activity assays. The nucleotide sequence has been determined. Features: • Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/myway/ Storage Conditions: Store at –20°C. Size Cat.# Price (Fr) 250 µg D1501 116.00 Part# 9PIG538 Size Cat.# Price (Fr) 100 µg G5381 584.00 Nucleic Acids Genomic DNA Product Human Genomic DNA: Male Human Genomic DNA: Female Human Genomic DNA Mouse Genomic DNA Size Cat.# Price (Fr) 100 µg G1471 301.00 100 µg G1521 301.00 100 µg G3041 242.00 100 µg G3091 242.00 G1471, G1521, G3041 For Laboratory Use. G3091 For Research Use Only. Not for Use in Diagnostic Procedures. Description: Genomic DNA from selected species are purified, and greater than 90% of the DNA is longer than 50kb in size as measured by pulsedfield gel electrophoresis. The DNA is suitable for Southern blot hybridizations, genomic analysis (including PCR), and genomic library construction. The Mouse Genomic DNA is isolated from whole blood from disease-free mice. Human Genomic DNA comes from multiple anonymous donors. Storage Conditions: Store at 4°C. Herring Sperm DNA Product Herring Sperm DNA Size Conc. Cat.# Price (Fr) 10 mg 10 µg/µl D1811 92.00 100 mg 10 µg/µl D1815 276.00 500 mg 10 µg/µl D1816 690.00 12 For complete and up-to-date product information visit: www.promega.com/catalog Pagina 15
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