Cell Signaling Unmethylated Lambda DNA Product Unmethylated Lambda DNA For Laboratory Use. Size Cat.# Price (Fr) 250 µg D1521 134.00 Description: Unmethylated cl857 Sam7 Lambda DNA (48,502bp) is isolated from infected GM119, an E. coli strain lacking both the dam and dcm methylase activities. Unmethylated Lambda DNA is used as a substrate for restriction enzymes sensitive to DNA methylation. Features: • Unmethylated Substrate: Use as a substrate for methylation-sensitive restriction enzymes. Storage Conditions: Store at –20°C. pBR322 Vector Product pBR322 Vector Size Conc. Cat.# Price (Fr) 10 µg 1 µg/µl D1511 116.00 For Research Use Only. Not for Use in Diagnostic Procedures. Description: The plasmid pBR322 Vector (4,361bp) carries the genes for tetracycline and ampicillin resistance. pBR322 DNA digests typically are used as molecular weight size markers in gel analysis of nucleic acids. Storage Conditions: Store at –20°C. AccI 3545 PstI 3611 PvuI 3736 ScaI 3846 Ampr ori PvuII 2066 (4,361bp) pBR322 Vector EcoRI 4359 HindIII 29 Tetr StyI 1369 BstZI 939 SalI 651 EcoRV 187 BamHI 375 SphI 566 K562 DNA High Molecular Weight Product K562 DNA High Molecular Weight For Research Use Only. Not for Use in Diagnostic Procedures. Description: K562 DNA is purified from a subculture of the human chronic myelogenous leukemia cell line. K562 DNA serves as a control for most steps of the single-locus probe analysis procedure. The DNA also can be used as a reference for determining fragment sizes of VNTR alleles following appropriate restriction digestion. K562 fragment sizes obtained may vary slightly due to interlaboratory differences in protocols and methods of analysis. Concentration: 0.4–1.0μg/μl. Storage Conditions: Store at –20°C. Always avoid multiple freeze-thaw cycles or exposure to frequent temperature changes. These fluctuations can greatly alter product stability. Size Cat.# Price (Fr) 30 µg DD2011 133.00 SacII 2863 SinI 2782 MluI 2147 φX174 (5,386bp) AccI 1193 HpaI 1292 DraI 1406 StuI 4489 ΦX174, RF DNA Product ΦX174, RF DNA For Laboratory Use. Size Conc. Cat.# Price (Fr) 50 µg 1 µg/µl D1531 194.00 Description: The icosahedral bacteriophage ΦX174 replicative form (RF) is a double-stranded circular DNA molecule of 5,386 bases. Restriction enzymedigested ΦX174 DNA generates molecular weight size markers used in gel analysis of nucleic acids. ΦX174 DNA is often used in the assays of restriction enzymes for the presence of nickase activity. Storage Conditions: Store at –20°C. HpaI 5002 AsuI 5042 PstI 1 HpaI 28 XhoI 163 1 MluI 222 DraI 327 AsuI 978 For complete and up-to-date product information visit: www.promega.com/catalog 13 Biochemicals and Labware 0270VA05_2A 0271VA04_1A Pagina 16

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