Science Catalog 2012 Life Cell Signaling Size Standards PowerPlex® 5-Dye Matrix Standards Product PowerPlex® 5-Dye Matrix Standards, 310 PowerPlex® 5-Dye Matrix Standards, 3100/3130 Not For Medical Diagnostic Use. Size Cat.# Price (Fr) 50 µl DG4600 162.00 25 µl DG4700 311.00 Description: The PowerPlex® 5-Dye Matrix Standards allow the PowerPlex® ESX, ESI and 18D Systems to be analyzed on the ABI PRISM® 310 Genetic Analyzer (Cat.# DG4600) and the ABI PRISM® 3100 and 3100-Avant or Applied Biosystems 3130, 3130xl, 3500 and 3500xL Genetic Analyzers (Cat.# DG4700). Proper generation of a spectral calibration file is critical to evaluate multicolor systems. The PowerPlex® 5-Dye Matrix Standards contain matrix fragments labeled with five fluorescent dyes: Fluorescein, JOE, TMR-ET, CXR-ET and CC5. Once generated, the spectral calibration file is applied during collection of PowerPlex® data to calculate and compensate for spectral overlap between different fluorescent dye colors. Storage Conditions: Store at –20°C. The matrix standards are light-sensitive; therefore, minimize light exposure. Protocol PowerPlex® 5-Dye Matrix Standards, 310 Technical Bulletin Part# TBD023 PowerPlex® 5-Dye Matrix Standards, 3100/3130 Technical Bulletin TBD024 PowerPlex® 4-Dye Matrix Standards Product PowerPlex® Matrix Standards, 310 PowerPlex® Matrix Standards, 3100/3130 Not For Medical Diagnostic Use. Size Cat.# Price (Fr) 50 µl DG4640 142.00 25 µl DG4650 318.00 Description: The PowerPlex® 4-Dye Matrix Standards allow the PowerPlex® 16, PowerPlex® 16 HS, PowerPlex® ES, PowerPlex® S5, PowerPlex® Y, PowerPlex® CS7, Custom, and PowerPlex® 16 and ES Monoplex Systems to be analyzed on the ABI PRISM® 310 Genetic Analyzer or ABI PRISM® 377 DNA Sequencer (Cat.# DG4640) and ABI PRISM® 3100 and 3100-Avant or Applied Biosystems 3130, 3130xl, 3500 and 3500xL Genetic Analyzers (Cat.# DG4650). Proper generation of a spectral calibration file is critical to evaluate multicolor systems. The PowerPlex® 4-Dye Matrix Standards contain matrix fragments labeled with four fluorescent dyes: Fluorescein, JOE, TMR and CXR. Once generated, the spectral calibration file is applied during collection of PowerPlex® data to calculate and compensate for spectral overlap between different fluorescent dye colors. Storage Conditions: Store at –20°C. The matrix standards are light-sensitive; therefore, minimize light exposure. Protocol PowerPlex® Matrix Standards, 310 Technical Bulletin PowerPlex® Matrix Standards, 3100/3130 Technical Bulletin Part# TBD021 TBD022 PowerPlex® Matrix Standards, 310/377 Product PowerPlex® Matrix Standards, 310/377 Not For Medical Diagnostic Use. Size Cat.# Price (Fr) 50 µl DG3640 142.00 Description: The PowerPlex® Matrix Standards, 310/377, allow the GenePrint® Fluorescent STR Systems to be run on the ABI PRISM® 310 Genetic Analyzer or ABI PRISM® 377 DNA Sequencer. Proper generation of a spectral calibration file is critical to evaluate multicolor systems. The PowerPlex® Matrix Standards, 310/377, contain matrix fragments labeled with five fluorescent dyes: Fluorescein, JOE A, JOE B, TMR and CXR. Once generated using the PowerPlex® dye set, the spectral calibration file is applied during the analysis of PowerPlex® data to calculate and compensate for the spectral overlap between different fluorescent dye colors. The PowerPlex® Matrix Standards, 310/377, contains two tubes of JOE, designated JOE A and JOE B. The PowerPlex® Matrix Standards, 3100 (Cat.# DG3650 and X3121), have been discontinued. Contact Promega Technical Services for more information. Protocol PowerPlex® Matrix Standards, 310/377 Technical Bulletin Internal Lane Standard 600 Product Internal Lane Standard 600 For Laboratory Use. Size Cat.# Price (Fr) 150 µl DG1071 472.00 Description: The Internal Lane Standard 600 (ILS 600) consists of 22 bands ranging in size from 60bp to 600bp. Fragments of 60–200bp are spaced at 20bp intervals, fragments of 200–500bp are spaced every 25 bases, and fragments of 500–600bp are spaced every 50 bases. Fragments that are multiples of 100 bases have fluorescence intensities approximately twice that of other fragments to simplify size assignment. The DNA ladder is double-stranded and asymmetrically labeled with carboxy-X-rhodamine (CXR). The Internal Lane Standard 600 is used to assign sizes to DNA fragments separated by electrophoresis and detected using a variety of fluorescence-detection instruments (e.g., Hitachi FMBIO® Fluorescence Imaging System and ABI PRISM® 310, 3100, 3100-Avant and Applied Biosystems 3130, 3130xl, 3500 and 3500xL Genetic Analyzers). ILS 600 is commonly used as an internal size marker for other applications and can be visualized by detecting fluorescent emission at 597nm after excitation at 576nm. In addition, the Internal Lane Standard 600 contains additives that prevent the formation of two artifacts (“split peak” and “n–10”) at the vWA locus in the PowerPlex® 16, 16 HS and PowerPlex® ES Systems when using ABI PRISM® 3100, 3100-Avant and Applied Biosystems 3130 and 3130xl Genetic Analyzers. Storage Conditions: Store at –20°C. Avoid multiple freeze-thaw cycles or exposure to frequent temperature changes. These fluctuations can greatly alter product stability. The Internal Lane Standard 600 is light-sensitive; therefore, minimize light exposure. Part# TBD018 158 For complete and up-to-date product information visit: www.promega.com/catalog Pagina 161
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