Cell Signaling STR Systems GenePrint® Fluorescent STR Systems Product GammaSTR™ Multiplex (Fluorescein) D16S539, D7S820, D13S317, D5S818 CSF1PO, TPOX, TH01, vWA Multiplex (Fluorescein) Available Separately CTTv Allelic Ladder Mix (Fluorescein) GammaSTR™ Allelic Ladder Mix (Fluorescein) 150 µl DG2121 1523.00 150 µl DG3291 1523.00 DG2121, DG3291 For Research Use Only. Not for Use in Diagnostic Procedures. DC6071, DC6070, DC6301, DC6300 Not For Medical Diagnostic Use. Description: The GenePrint ® Fluorescent STR Systems were developed for use with the Hitachi FMBIO® Fluorescence Imaging Systems and ABI PRISM® 377 DNA Sequencer. One primer for each locus is labeled with fluorescein to allow fluorescent detection. Fluorescein has an excitation maximum at 488nm and an emission maximum at 532nm. Therefore, the systems are compatible with a variety of fluorescence-detection instruments, including the ABI PRISM® 310, 3100 and 3100-Avant Genetic Analyzers and Applied Biosystems 3130 and 3130xl Genetic Analyzers. Each system provides all materials required to amplify STR regions of purified genomic DNA except Taq DNA polymerase. Amplification of DNA using the system components plus Taq DNA polymerase produces fluorescein-labeled fragments representing alleles from the template DNA. For instruments that support two-color fluorescence detection, additional precision may be achieved by including the Internal Lane Standard 600 (Cat.# DG1071) in each gel lane or injection. Features: • High-Throughput Analysis: Analysis is achieved by comparing amplified DNA fragments directly with the allelic ladder provided for each locus. • Efficiency: The fluorescent STR multiplex systems support simultaneous single-tube amplification of four polymorphic STR loci with nonoverlapping allele size ranges. • Allelic Ladders: Comparing amplified alleles with allelic ladders provided with each system allows rapid and reliable allele assignment. Storage Conditions: Store at –20°C. The fluorescent primer pairs and allelic ladders are light-sensitive; therefore, minimize light exposure. Protocol GenePrint ® Fluorescent STR Systems Technical Manual Part# TMD006 Available Separately CTT Allelic Ladder Mix FFv Allelic Ladder Mix Size Cat.# Price (Fr) 100 reactions DC6071 1352.00 400 reactions DC6070 4732.00 100 reactions DC6301 1352.00 400 reactions DC6300 4732.00 GenePrint® STR Systems (Silver Stain Detection) Product GenePrint ® SilverSTR® III System (D7S820, D13S317, D16S539) CSF1PO, TPOX, TH01 Multiplex F13A01, FESFPS, vWA Multiplex Size Cat.# Price (Fr) 100 reactions DC6451 1325.00 400 reactions DC6450 4638.00 100 reactions DC6001 1325.00 400 reactions DC6000 4638.00 100 reactions DC6031 1325.00 400 reactions DC6030 4638.00 150 µl DG2101 1066.00 150 µl DG2141 1066.00 DG2101, DG2141 For Research Use Only. Not for Use in Diagnostic Procedures. DC6451, DC6450, DC6001, DC6000, DC6031, DC6030 Not For Medical Diagnostic Use. Description: The GenePrint ® Silver STR Systems provide a rapid, nonradioactive method to evaluate small amounts (e.g., 1ng) of human DNA. The systems provide all materials required to amplify STR regions of purified genomic DNA except for Taq DNA polymerase and sample DNA. The amplified STR fragments are separated by polyacrylamide gel electrophoresis and detected by silver staining. The combination of SilverSTR® III, CTT and FFv provides access to seven of the thirteen core loci that comprise the Combined DNA Index System (CODIS) database. Features: • Economical: The GenePrint ® Silver STR Systems do not require fluorescence-detection equipment for analysis. The systems are ideal for labs that are starting STR analysis or do not wish to purchase expensive fluorescence-detection equipment. • Efficient: Analysis requires less than one day. Each multiplex allows simultaneous amplification of three nonoverlapping STR loci for high discrimination power. Storage Conditions: Store at –20°C. Protocol GenePrint ® STR Systems Technical Manual Sex Identification Systems GenePrint® Sex Identification Systems Product Amelogenin (Fluorescein Detection) Amelogenin (TMR) Not For Medical Diagnostic Use. Description: The GenePrint ® Sex Identification Systems can be used for sex determination. When used under reaction conditions recommended in the Technical Manuals (#TMD004, #TMD006 and #TMD008), a specific segment of the human X chromosome generates a 212bp product, while the corresponding human Y-chromosomal DNA segment produces a 218bp fragment. The Amelogenin locus may be co-amplified and co-analyzed with a compatible multiplex system by mixing the Amelogenin primers with those of the appropriate multiplex system prior to use. Storage Conditions: Store at –20°C. The fluorescent primer pair and ladder are light-sensitive; therefore, minimize light exposure. Protocol GenePrint ® Fluorescent STR Systems Technical Manual PowerPlex® 1.1 System Technical Manual Part# TMD006 TMD008 Size Cat.# Price (Fr) 100 reactions DC5171 414.00 100 reactions DC6171 414.00 Part# TMD004 7 For complete and up-to-date product information visit: www.promega.com/catalog 159 Genetic Identity Pagina 162
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