Science Catalog 2012 Life Cell Signaling Biochemical Reagents Gold ST★R 10X Buffer Product Gold ST★R 10X Buffer For Research Use Only. Not for Use in Diagnostic Procedures. Description: Gold ST★R 10X Buffer can be used to amplify STR loci using AmpliTaq Gold® DNA polymerase. Gold ST★R Buffer can be substituted for the STR 10X Buffer, which is supplied with PowerPlex® and GenePrint ® STR Systems, allowing the use of either AmpliTaq® or AmpliTaq Gold® DNA polymerase. This buffer includes BSA for a more robust reaction and improved results under nonoptimal conditions. The combination of Gold ST★R 10X Buffer and AmpliTaq Gold® DNA polymerase can result in greater sensitivity and reduced amplification artifacts. Storage Conditions: Store at –20°C. 2800M Control DNA Product 2800M Control DNA Not For Medical Diagnostic Use. Description: The 2800M Control DNA is a single-source male human genomic DNA. This DNA can be used as a control for human STR analysis. Storage Conditions: Store at 2–10°C. K562 DNA High Molecular Weight Product K562 DNA High Molecular Weight For Research Use Only. Not for Use in Diagnostic Procedures. Description: K562 DNA is purified from a subculture of the human chronic myelogenous leukemia cell line. K562 DNA serves as a control for most steps of the single-locus probe analysis procedure. The DNA also can be used as a reference for determining fragment sizes of VNTR alleles following appropriate restriction digestion. K562 fragment sizes obtained may vary slightly due to interlaboratory differences in protocols and methods of analysis. Concentration: 0.4–1.0μg/μl. Storage Conditions: Store at –20°C. Avoid multiple freeze-thaw cycles or exposure to frequent temperature changes. These fluctuations can greatly alter product stability. Size Cat.# Price (Fr) 30 µg DD2011 133.00 Size Conc. 25 µl 10 ng/µl Cat.# Price (Fr) DD7101 160.00 500 µl 0.25 ng/µl DD7251 Pls. Enq. Size Cat.# Price (Fr) 1.2 ml DM2411 74.00 Biochemical Reagents Product SILVER SEQUENCE™ Staining Reagents STR 10X Buffer Gold ST★R 10X Buffer Agarose STR 2X Loading Solution Blue Dextran Loading Solution Gel Tracking Dye Bromophenol Blue Loading Solution Mineral Oil Size Cat.# Price (Fr) 10 gels Q4132 310.00 1.2 ml DM2211 74.00 1.2 ml DM2411 74.00 1 kg DV3123 1290.00 3 ml DV4331 74.00 3 × 1 ml DV4351 74.00 4 × 250 µl DV4361 57.00 3 × 1 ml DV4371 74.00 12 ml DY1151 53.00 DV4351, DV4361, DV4371 For Laboratory Use. Q4132, DM2211, DM2411, DV3123, DV4331, DY1151 For Research Use Only. Not for Use in Diagnostic Procedures. Description: Promega offers supporting reagents for separation, hybridization and detection of specific loci in the human genome. These quality-tested reagents are optimized for use with Promega genetic identity systems. Storage Conditions: Store Cat.# DM2211, DM2411, DV4331, DV4351, DV4361 and DV4371 at –20°C. Store Cat.# Q4132, DV3123 and DY1151 at 22–25°C. Protocol Part# SILVER SEQUENCE™ DNA Sequencing System Technical Manual TM023 160 For complete and up-to-date product information visit: www.promega.com/catalog Pagina 163

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