Cell Signaling HaloTag® Ligand Building Blocks Product HaloTag® Amine (O4) Ligand HaloTag® Amine (O2) Ligand HaloTag® Iodoacetamide (O4) Ligand HaloTag® Iodoacetamide (O2) Ligand HaloTag® Succinimidyl Ester (O4) Ligand HaloTag® Succinimidyl Ester (O2) Ligand HaloTag® Thiol (O4) Ligand For Research Use Only. Not for Use in Diagnostic Procedures. Description: The HaloTag® Ligand Building Blocks can carry a variety of functionalities, including fluorescent labels, affinity tags and attachments to a solid phase. The covalent bond forms rapidly under general physiological conditions, is highly specific and essentially irreversible. The HaloTag® Ligand Building Blocks allow researchers to apply the chloroalkane group that HaloTag® protein reacts with to any compound or surface with a compatible chemical group, creating endless possible applications. The HaloTag® Succinimidyl Ester (04) Ligand contains a reactive succinimidyl ester (SE) group connected to an alkyl chloride separated by three ethylene glycol repeats (04). The HaloTag® Succinimidyl Ester (04) Ligand can be successfully conjugated to any reporter group, protein or nucleic acid derivative containing an amine, forming stable amide bond linkages. The ligand with functional group can then be used with the HaloTag® protein for any application of interest. The HaloTag® Succinimidyl Ester (O2) Ligand contains a reactive succinimidyl ester (SE) group connected to an alkylchloride separated by an ethylene glycol repeat (O2). The HaloTag® Succinimidyl Ester (O2) Ligand can be successfully conjugated to any reporter group, protein or nucleic acid derivative containing an amine, forming stable amide bond linkages. The ligand with functional group can then be used with the HaloTag® protein for any application of interest. The HaloTag® Amine (04) Ligand contains a reactive amine group connected to an alkyl chloride, separated by an ethylene glycol repeat (04). The HaloTag® Amine (04) Ligand can be successfully conjugated to any reporter group, protein, or nucleic acid derivative containing an activated carboxylic acid, sulfonyl halide or isocyanate. Examples of activated carboxylic acids are succinimidyl esters, STP esters, acid halides and TFP esters. The ligand with functional group can then be used with the HaloTag® protein for any application of interest. The HaloTag® Amine (O2) Ligand contains a reactive amine group connected to an alkylchloride, separated by an ethylene glycol repeat (O2). The HaloTag® Amine (O2) Ligand can be successfully conjugated to any reporter group, protein or nucleic acid derivative containing an activated carboxylic acid, sulfonyl halide or isocyanate. Examples of activated carboxylic acids are succinimidyl esters, STP esters, acid halides and TFP esters. The ligand with functional group can then be used with the HaloTag® protein for any application of interest. The HaloTag® Iodoacetamide (04) Ligand contains a reactive iodoacetamide group connected an alkyl chloride separated by an ethylene glycol repeat (04). The HaloTag® Iodoacetamide (04) Ligand has been designed to rapidly react with sulfhydryl-containing molecules, whether small organic compounds, peptides or proteins. The ligand with functional group can then be used with the HaloTag® protein for any application of interest. The HaloTag® Iodoacetamide (O2) Ligand contains a reactive iodoacetamide group connected to an alkylchloride separated by an ethylene glycol repeat (O2). HaloTag® Iodoacetamide (O2) Ligand has been designed to rapidly react with sulfhydryl-containing molecules, whether small organic compounds, peptides or proteins. The ligand with functional group can then be used with the HaloTag® protein for any application of interest. The HaloTag® Thiol (O4) Ligand contains a reactive sulfhydryl group connected to an alkyl chloride separated by three ethylene glycol repeats (04). The HaloTag® Thiol (04) Ligand can be successfully conjugated to any reporter group, cross-linking reagent (bound or free), or nucleic acid derivative containing a number of different alkylating groups, forming stable thioether bonds. Commonly used reagents that rapidly react with sulfhydryls include iodo- or bromo-acetyls or benzyls, bromo- or chloro-mustards, maleimides, For complete and up-to-date product information visit: www.promega.com/catalog 163 Size Cat.# Price (Fr) 5 mg P6741 831.00 5 mg P6711 831.00 5 mg P6771 831.00 5 mg P1681 831.00 5 mg P6751 831.00 5 mg P1691 831.00 5 mg P6761 831.00 aziridines, acryloyl derivatives, and halide or sulfonate containing arenes (those bearing Electron Withdrawing Groups (EWGs) react most rapidly). The reactive ligand can be captured with HaloTag® protein either before or after the thiol group is functionalized for any application of interest. Storage Conditions: Store Cat.# P1691 and P6751 at or below –70°C under inert atmosphere. Store Cat.# P6711 and P6741 at or below –20°C in an air-tight container in the absence of light. Store Cat.# P1681, P6771 and P6761 at or below –20°C under inert atmosphere in the absence of light. See Promega Product Information for additional details on individual products. Anti-HaloTag® pAb Product Anti-HaloTag® pAb Size Conc. For Research Use Only. Not for Use in Diagnostic Procedures. Description: The Anti-HaloTag® pAb is a purified rabbit polyclonal antibody raised against the HaloTag® protein. The antibody is purified using Protein G affinity resin and supplied at 1mg/ml in PBS. The antibody detects HaloTag® fusion proteins in Western blot hybridization and immunocytochemistry applications with high sensitivity and specificity. The HaloTag® protein is not endogenous to mammalian, plant and E. coli cells. E. coli and mammalian cell extracts demonstrate low cross-reactivity with the Anti-HaloTag® pAb. Features: • Specificity: The Anti-HaloTag® pAb is specific for HaloTag® protein and exhibits low cross-reactivity with E. coli and mammalian cell extracts. Storage Conditions: Store at –20°C. Protocol HaloTag® Technology: Focus on Imaging Technical Manual HaloTag® Fusion (C-Terminal) Mammalian Expression Vectors Product pHTC HaloTag® CMV-neo Vector pFC27A HaloTag® CMV-neo Flexi® Vector pFC27K HaloTag® CMV-neo Flexi® Vector pFC14A HaloTag® CMV Flexi® Vector pFC14K HaloTag® CMV Flexi® Vector pFC15A HaloTag® CMVd1 Flexi® Vector pFC15K HaloTag® CMVd1 Flexi® Vector pFC16A HaloTag® CMVd2 Flexi® Vector pFC16K HaloTag® CMVd2 Flexi® Vector pFC17A HaloTag® CMVd3 Flexi® Vector pFC17K HaloTag® CMVd3 Flexi® Vector For Research Use Only. Not for Use in Diagnostic Procedures. Size Cat.# Price (Fr) 20 µg G7711 Pls. Enq. 20 µg G8421 Pls. Enq. 20 µg G8431 Pls. Enq. 20 µg G9651 399.00 20 µg G9661 399.00 20 µg G1611 399.00 20 µg G1601 399.00 20 µg G1591 399.00 20 µg G1571 399.00 20 µg G1551 399.00 20 µg G1321 399.00 Part# TM260 Cat.# Price (Fr) 200 µg 1 mg/ml G9281 444.00 8 Imaging and Immunological Detection Pagina 166
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