Science Catalog 2012 Life Cell Signaling HaloTag® Fusion (N-Terminal) Mammalian Expression Vectors Product pHTN HaloTag® CMV-neo Vector pFN28A HaloTag® CMV-neo Flexi® Vector pFN28K HaloTag® CMV-neo Flexi® Vector pFN21A HaloTag® CMV Flexi® Vector pFN21K HaloTag® CMV Flexi® Vector pFN22A HaloTag® CMVd1 Flexi® Vector pFN22K HaloTag® CMVd1 Flexi® Vector pFN23A HaloTag® CMVd2 Flexi® Vector pFN23K HaloTag® CMVd2 Flexi® Vector pFN24A HaloTag® CMVd3 Flexi® Vector pFN24K HaloTag® CMVd3 Flexi® Vector For Research Use Only. Not for Use in Diagnostic Procedures. For additional information see page 238. ELISAs and Antibodies BDNF Emax® ImmunoAssay Systems Product BDNF Emax® ImmunoAssay System For Research Use Only. Not for Use in Diagnostic Procedures. Description: The BDNF Emax® ImmunoAssay Systems provide optimized reagents and a protocol for the sensitive and specific detection of brainderived neurotrophic factor (BDNF). After an overnight coating of a 96-well plate, the specific protein is detected using an antibody sandwich format. The systems use a horseradish peroxidase-conjugated secondary antibody and a single-component TMB substrate for the final chromogenic detection of bound neurotrophic factor. Using this system, BDNF in tissue culture supernatants, tissue homogenates, plasma and urine can be quantitated in the range of 7.8–500pg/ml. Binding and recovery from mouse brain homogenates has not been fully characterized. Features: • High Value: Optimized reagents and protocol provided. • Specificity: Specific detection of BDNF; less than 3% cross-reactivity with other related neurotrophic and growth factors. • Sensitivity: Detect picogram levels of factor per milliliter of sample. • Flexibility: Available in sizes for two or five 96-well plates; can configure plates as desired. Storage Conditions: Store the entire system in its original package protected from light at –20°C. Protocol BDNF Emax® ImmunoAssay System Technical Bulletin Part# TB257 Size Cat.# Price (Fr) 2 × 96 wells G7610 786.00 5 × 96 wells G7611 1572.00 NGF Emax® ImmunoAssay Systems Product NGF Emax® ImmunoAssay System For Research Use Only. Not for Use in Diagnostic Procedures. Description: The NGF Emax® ImmunoAssay Systems provide optimized reagents and a protocol for the sensitive and specific detection of biologically active nerve growth factor (NGF). After an overnight coating of a 96-well plate, the specific protein is detected using an antibody sandwich format. The systems use horseradish peroxidase-conjugated secondary antibody and a single-component TMB substrate for the final chromogenic detection of bound neurotrophic factor. The system can be used to quantitate NGF in tissue culture supernatants and tissue extracts in the range of 3.9–250pg/ml. Avoid using samples containing high levels of IgG such as serum, plasma and spleen. Features: • High Value: Optimized reagents and protocol provided. • Specificity: Specific detection of NGF; less than 3% cross-reactivity with other related neurotrophic and growth factors. • Sensitivity: Detect picogram levels of factor per milliliter of sample. • Flexibility: Available in sizes for two or five 96-well plates; can configure plates as desired. Storage Conditions: Store the entire system in its original package protected from light at –20°C. Protocol NGF Emax® ImmunoAssay System Technical Bulletin Part# TB226 Size Cat.# Price (Fr) 2 × 96 wells G7630 786.00 5 × 96 wells G7631 1572.00 Size Cat.# Price (Fr) 20 µg G7721 Pls. Enq. 20 µg G8441 Pls. Enq. 20 µg G8451 Pls. Enq. 20 µg G2821 399.00 20 µg G2831 399.00 20 µg G2841 399.00 20 µg G2851 399.00 20 µg G2861 399.00 20 µg G2871 399.00 20 µg G2881 399.00 20 µg G2981 399.00 GDNF Emax® ImmunoAssay Systems Product GDNF Emax® ImmunoAssay System For Research Use Only. Not for Use in Diagnostic Procedures. Description: The GDNF Emax® ImmunoAssay Systems provide optimized reagents and a protocol for the sensitive and specific detection of glial cellline-derived neurotrophic factor (GDNF). After an overnight coating of a 96-well plate, the specific protein is detected using an antibody sandwich format. The systems use horseradish peroxidase-conjugated secondary antibody and a single-component TMB substrate for the final chromogenic detection of bound neurotrophic factor. Using this system, GDNF in tissue culture supernatants or tissue homogenates can be quantitated in the range of 15.6–1,000pg/ml. Features: • High Value: Optimized reagents and protocol provided. • Specificity: Specific detection of GDNF; less than 3% cross-reactivity with other related neurotrophic and growth factors. • Sensitivity: Detect picogram levels of factor per milliliter of sample. • Flexibility: Available in sizes for two or five 96-well plates; can configure plates as desired. Storage Conditions: Store the entire system in its original package protected from light at –20°C. Once thawed, store the system (except the GDNF Standard) at 4°C. Protocol GDNF Emax® Immunoassay System Technical Bulletin Part# TB221 Size Cat.# Price (Fr) 2 × 96 wells G7620 786.00 5 × 96 wells G7621 1572.00 164 For complete and up-to-date product information visit: www.promega.com/catalog Pagina 167
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