Cell Signaling Block & Sample 5X Buffer Product Block & Sample 5X Buffer For Laboratory Use. Size Cat.# Price (Fr) 54 ml G3311 84.00 Description: The Block & Sample 5X Buffer is optimized for use in the Emax® ImmunoAssay Systems (for BDNF, GDNF and NGF) providing additional buffer for further sample dilutions and manipulations. This buffer is used to block the plates and dilute the standards, samples, detection antibodies and conjugates in these Emax® ImmunoAssay Systems. The buffer is provided as 54ml of buffer containing gentamicin as a preservative. Note: The Block & Sample 5X Buffer should not be used with the TGFβ1 Emax® ImmunoAssay System.Storage Conditions: Store at 4°C. TGFβ1 Emax® ImmunoAssay Systems Product TGFβ1 Emax® ImmunoAssay System For Research Use Only. Not for Use in Diagnostic Procedures. Description: The TGFβ1 Emax® ImmunoAssay System provides optimized reagents and a protocol for the sensitive and specific detection of transforming growth factor β1 (TGFβ1). After an overnight coating of a 96-well plate, the specific protein is detected using an antibody sandwich format. The system uses horseradish peroxidase-conjugated secondary antibody and a singlecomponent TMB substrate for the final chromogenic detection of bound TGFβ1. Using this system, biologically active TGFβ1 in tissue culture supernatants, plasma, serum or urine can be quantitated in the range of 15.6–1,000pg/ml. Features: • High Value: Optimized reagents and protocol provided. • Specificity: Specific detection of TGFβ1; less than 3% cross-reactivity with other related growth factors (TGFβ2 and TGFβ3). • Sensitivity: Detect picogram levels of factor per milliliter of sample. • Flexibility: Available in sizes for two or five 96-well plates; can configure plates as desired. Storage Conditions: Store the entire system in its original package protected from light at –20°C. Protocol TGFß1 Emax® ImmunoAssay System Technical Bulletin TGFβ Sample 10X Buffer Product TGFβ Sample 10X Buffer For Research Use Only. Not for Use in Diagnostic Procedures. Description: The TGFβ Sample 10X Buffer is an optimized proprietary buffer designed for use with the TGFβ1 Emax® ImmunoAssay System to reduce high background, a common problem with traditional buffers used in TGFβ ELISAs. Storage Conditions: Store at 4°C. Size Cat.# Price (Fr) 20 ml G1291 80.00 Part# TB196 Immunocytochemical staining of Akt in embryonic rat brain cells. Embryonic (day 17) rat brain cells were collected and treated with 20ng/ml each of EGF and FGF. Anti-pS473 Akt pAb was used at a 1:50 dilution. Positive cells were visualized using a donkey anti-rabbit, Cy®3-conjugated secondary antibody. Nuclei were stained using DAPI. Protocols developed and performed at Promega. Size Cat.# Price (Fr) 2 × 96 wells G7590 786.00 5 × 96 wells G7591 1572.00 Anti-pS473 Akt pAb Product Anti-pS473 Akt pAb For Research Use Only. Not for Use in Diagnostic Procedures. Description: Anti-pS473 Akt pAb is an affinity-purified polyclonal rabbit antibody. The antibody is purified using a phosphorylated peptide that corresponds to the phospho-S473 form of Akt-1 and is useful for both Western blotting and immunocytochemistry. Features: • Specificity: The antibody is selective for the Ser473 phosphorylated isoforms of Akt and does not show cross-reactivity with nonphosphorylated Akt. • Immunogen: Peptide from the singly phosphorylated Ser473 region from the C-terminus of Akt-1 protein. • Antibody Form: Affinity-purified rabbit IgG, supplied in PBS with 50μg/ml gentamicin. • Value: Will generate 100ml of blotting solution, sufficient for 10 Western blots of 10ml each. Storage Conditions: Store at 4°C for daily/weekly use or dispense into aliquots and store at –20°C for long-term storage. Protocol Anti-pS473 Akt pAb Product Information Part# 9PIG744 Size Cat.# Price (Fr) 40 µl G7441 677.00 8 For complete and up-to-date product information visit: www.promega.com/catalog 165 Imaging and Immunological Detection 2769TA10_9A Pagina 168
Pagina 170Interactieve erapport, deze nieuwsbrief of pdf is levensecht online geplaatst met Online Touch en bied het online bladerbaar maken van digi-verenigingsbladen.
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