Cell Signaling Anti-ERK 1/2 pAb, Rabbit Product Anti-ERK 1/2 pAb, Rabbit For Research Use Only. Not for Use in Diagnostic Procedures. Description: Anti-ERK 1/2 pAb is a polyclonal antibody purified from rabbit serum. The antibody is affinity-purified using a peptide sequence in human/ rat ERK1. Features: • Specificity: Detects ERK1 and ERK2 in the nonphosphorylated, monophosphorylated and dually phosphorylated forms. • Immunogen: Sequence representing a conserved region in human and rat ERK1 located outside of the catalytic core of the enzyme. • Antibody Form: Affinity-purified rabbit IgG; supplied in PBS (pH 7.4). • Value: When used at the recommended 1:5,000 dilution, this product will generate up to 200ml of blotting solution, sufficient for 20 Western blots of 10ml each. Storage Conditions: Store at –20°C. Protocol Anti-ERK 1/2 pAb, Rabbit Product Information Anti-ACTIVE® MAPK pAb kDa 98 – 64 – 50 – Anti-ERK 1/2 pAb Part# 9PIV114 Size Cat.# Price (Fr) 40 µl V1141 388.00 Anti-Human BDNF pAb Product Anti-Human BDNF pAb For Research Use Only. Not for Use in Diagnostic Procedures. Description: BDNF, a 27kDa homodimer originally derived from human brain, shares high sequence homology with NGF, NT-3 and NT-4/5 and influences many neuron types in the CNS. Anti-Human BDNF pAb is generated in chickens and purified using a proprietary polyethylene glycol procedure. IgY, the 180kDa chicken IgG homolog, can be produced in chickens against certain biological antigens that fail to elicit a humoral immune response in rabbits or other mammals due to species relatedness. This antibody is highly specific for BDNF. Features: • Immunogen: Human recombinant BDNF. • Antibody Form: Chicken IgY, provided at 0.5mg/ml in 0.1M NaCl, 0.01M K2HPO4 and 50μg/ml gentamicin. • Specificity: Cross-reactive between mammalian species; does not cross-react with other neurotrophic factors. Storage Conditions: Store at 4°C. Size Cat.# Price (Fr) 200 µg G1641 791.00 8 36 – 1 2 1 2 Detection of the specifically phosphorylated form of MAPK in NGFtreated PC12 cell extracts. Anti-ACTIVE® MAPK pAb (Cat.# V8031) and Anti-ERK 1/2 (“pan ERK 1/2”) pAb (Cat.# V1141) detection of ERK 1/2 in untreated (lanes 1) or NGF-treated (lanes 2) PC12 cell extracts (2μg). Localization of BDNF in primary cultures of hippocampal neurons. The Anti-Human BDNF pAb was used at a 1:200 dilution. Primary antibody was detected using HRP-conjugated goat anti-chicken IgY secondary antibody. Photomicrograph kindly provided by Dr. Laurie Goodman, Lynx Therapeutics, Hayward, CA. Reprinted by permission of Academic Press, Goodman, L. et al. (1996) Mol. Cell Neurosci. 7, 222. For complete and up-to-date product information visit: www.promega.com/catalog 169 Imaging and Immunological Detection 2582TB03_9A 1830TA07_7A Pagina 172
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