Science Catalog 2012 Life Cell Signaling Anti-ACTIVE® Caspase-3 pAb Product Anti-ACTIVE® Caspase-3 pAb For Research Use Only. Not for Use in Diagnostic Procedures. Description: Anti-ACTIVE® Caspase-3 pAb is intended for use as a marker of apoptosis; it specifically stains apoptotic cells without staining nonapoptotic cells. Includes sufficient antibody to perform 125 immunocytochemical assays (100μl/assay) at a 1:250 dilution. Features: • Immunogen: Peptide derived from the p17 fragment of caspase-3 and having sequence homology in human, mouse, rat and hamster. • Antibody Form: Affinity-purified rabbit IgG; supplied in Dulbecco’s PBS. • Specificity: Specifically recognizes the cleaved active form of caspase-3 in human, rat and mouse. Storage Conditions: Store at –20°C. Protocol Anti-ACTIVE® Caspase-3 pAb Product Information Part# 9PIG748 Size Cat.# Price (Fr) 50 µl G7481 616.00 Product Anti-β-Galactosidase mAb Size Anti-β-Galactosidase, Purified Monoclonal Antibody For Laboratory Use. Description: This antibody [subclass IgG2a(κ)] was purified from ascites of a mouse hybridoma and recognizes E. coli β-galactosidase. Features: • Immunogen: β-galactosidase. • Antibody Form: 2.0–2.5mg/ml in 10mM Tris-HCl (pH 8.0), 150mM NaCl, 0.02% sodium azide. • Specificity: E. coli β-galactosidase near the C-terminal end. Storage Conditions: Store undiluted at –20°C. A. B. Conc. Cat.# Price (Fr) 100 µg 2.0–2.5 mg/ml Z3781 69.00 2 mg 2.0–2.5 mg/ml Z3783 690.00 C. D. E. F. Demonstration of Anti-ACTIVE® Caspase-3 pAb positive cells in postnatal day 0 (P0) mouse brain paraffin-embedded sections. Panel A. Three Anti-ACTIVE® Caspase-3 pAb-positive cells (colored arrows). Panel B. Corresponding DAPI-stained nuclei. Note the correspondence of Anti-ACTIVE® Caspase-3 pAb label with the typical apoptotic, condensed nuclear morphology in Panel B. Protocols developed and performed at Promega. Histological analysis of axonal termination in the accessory olfactory bulb (AOB). Sagittal sections through the AOB stained with the Anti-β-Galactosidase mAb (Cat.# Z3781; green) and antibodies against synaptophysin (DAKO, red). DAPI staining is shown in blue. Panel A. Heterozygous VL mouse. Panels B and C. Homozygous VL mouse. Panel D. Homozygous ΔV mouse. Panel E. Heterozygous MV mouse. Panel F. Homozygous VG mouse. Details on gene targeting, mutations and immunostaining may be found in Rodriguez, J., Feinstein, P. and Mombaerts, P. (1999) Cell 97, 199. Images kindly provided by Dr. Peter Mombaerts, The Rockefeller University, New York. Reprinted by permission of Cell Press. 170 For complete and up-to-date product information visit: www.promega.com/catalog 3158TB 2761TA10_9A Pagina 173
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