Cell Signaling Anti-Human GDNF pAb Product Anti-Human GDNF pAb For Research Use Only. Not for Use in Diagnostic Procedures. Description: Human glial cell-line-derived neurotrophic factor (GDNF), a 30kDa homodimer, has been shown to be a potent survival factor for a variety of neurons. The receptor complex for GDNF has been elucidated, though members of the multicomponent receptor family continue to grow. With applications in Western blotting, ELISA and immunostaining, the Anti-Human GDNF pAb is a useful tool to continue the investigation of GDNF’s role in multiple facets of neurological systems. Features: • Immunogen: Human recombinant GDNF. • Antibody Form: Chicken IgY; 0.5mg/ml in 0.1M NaCl, 0.01M K2HPO4, 50μg/ml gentamicin. • Specificity: Cross-reactive between mammalian species; does not cross-react with TGFα, TGFβ1, NGF or BDNF at up to 10μg/ml. Storage Conditions: Store at 4°C. Anti-GFAP pAb Product Anti-GFAP pAb For Research Use Only. Not for Use in Diagnostic Procedures. Description: Anti-GFAP pAb is a polyclonal antibody against glial fibrillary acidic protein (GFAP), a specific marker of astrocytes in the central nervous system, and is qualified for immunostaining applications. Features: • Immunogen: Purified glial fibrillary acidic protein from bovine spinal cord. • Antibody Form: Purified rabbit IgG; supplied at 1mg/ml in PBS containing 50μg/ml gentamicin. • Specificity: Human, bovine and rat GFAP; not recommended for mouse. Storage Conditions: Store at 4°C. NIH/3T3 cells transiently transfected with a luciferase gene. Luciferase-expressing cells were detected using the Anti-Luciferase pAb (Cat.# G7451). Protocols developed and performed at Promega. Size Cat.# Price (Fr) 100 µg G5601 376.00 Size Cat.# Price (Fr) 200 µg G2791 760.00 Anti-Luciferase pAb Product Anti-Luciferase pAb For Research Use Only. Not for Use in Diagnostic Procedures. Description: Anti-Luciferase pAb is a goat polyclonal antibody designed for use in immunocytochemistry and Western blot applications. Anti-Luciferase pAb can detect luciferase enzyme expression in situ. Features: • Immunogen: Recombinant luciferase from North American firefly (Photinus pyralis). • Antibody Form: Goat polyclonal IgG at 1mg/ml in PBS containing 50μg/ml gentamicin. • Specificity: Anti-Luciferase pAb is specific for firefly luciferase (Photinus pyralis) and does not cross-react with sea pansy (Renilla reniformis) luciferase. Storage Conditions: Store at 4°C. Size Cat.# Price (Fr) 200 µg G7451 407.00 8 Anti-GFAP-labeled astrocytes in mixed rat neural progenitor cultures. Cells were labeled with DAPI (blue) and Anti-GFAP pAb with a Cy®3-conjugated secondary antibody (red). Protocols developed and performed at Promega. For complete and up-to-date product information visit: www.promega.com/catalog 171 Imaging and Immunological Detection 2773TA10_9A 2760TA10_9A Pagina 174
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