Science Catalog 2012 Life Cell Signaling Anti-NGF mAb Product Anti-NGF mAb For Research Use Only. Not for Use in Diagnostic Procedures. Description: Nerve growth factor (NGF) is a member of the neurotrophin family of growth factors. NGF is expressed in sympathetic and sensoryinnervated peripheral tissues and mediates phosphorylation of specific intracellular proteins. At the cellular level, NGF expression has been demonstrated in lymphocytes, smooth muscle cells, epithelial cells, astrocytes, fibroblasts and Schwann cells. Anti-NGF mAb was designed as a specific marker of NGF in Western blotting, ELISA and immunostaining applications. Features: • Immunogen: Purified murine NGF, 2.5S. • Antibody Form: Rat IgG (clone 1G3) provided at 1mg/ml as frozen liquid in PBS containing no preservatives. • Specificity: Reacts with human NGF, 2.5S mNGF and to a lesser extent with 7S mNGF. Cross-reacts between mammalian species. • Activity: The Anti-NGF mAb exhibits a half-maximal titer of less than or equal to 250ng/ml in an ELISA protocol using 100ng of 2.5S mNGF (Cat.# G5141). Storage Conditions: Store at –20°C. Size Cat.# Price (Fr) 20 µg G1132 232.00 100 µg G1131 928.00 Anti-Human NT-3 pAb Product Anti-Human NT-3 pAb For Research Use Only. Not for Use in Diagnostic Procedures. Description: Neurotrophin-3, a 27kDa homodimer that shares high sequence homology with NGF, BDNF, NT-4 and NT-5, influences many neuron types in the central and peripheral nervous system. NT-3 is also highly conserved across species. Anti-Human NT-3 pAb is generated in chickens and purified using a proprietary polyethylene glycol procedure. IgY, the 180kDa chicken IgG homolog, can be produced in chickens against certain biological antigens that fail to elicit a humoral immune response in rabbits or other mammals due to species relatedness. This antibody is highly specific for NT-3 in a variety of mammalian species. Features: • Immunogen: Human recombinant NT-3. • Antibody Form: Chicken IgY is provided at 0.5mg/ml in 0.1M NaCl, 0.01M K2HPO4 and 50μg/ml gentamicin. • Specificity: Cross-reactive with human and mouse NT-3 and is presumed to cross-react with rat and Rhesus monkey NT-3 based on factor sequence identity across species; does not cross-react with BDNF or NGF and has limited cross-reactivity to NT-4. Storage Conditions: Store at 4°C. Size Cat.# Price (Fr) 200 µg G1651 791.00 Rat basal forebrain cholinergic neuron stained with Anti-NGF mAb following intraventricular injection of 30µg of NGF and factor uptake. Photomicrograph kindly provided by Dr. Charles Howe, University of California, San Francisco. Immunofluorescent detection of NT-3 in monocyte-derived macrophages and purified human fetal microglia. Chicken Anti-Human NT-3 pAb in red and RCA-1 (macrophage marker) in green. Image kindly provided by Drs. Pam Sarnacki, Wanda Wang and Chris Achim, University of Pittsburgh. . 172 For complete and up-to-date product information visit: www.promega.com/catalog 1283TA12_5B 1411TA03_6A Pagina 175
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