Cell Signaling Anti-PARP p85 Fragment pAb Product Anti-PARP p85 Fragment pAb For Research Use Only. Not for Use in Diagnostic Procedures. Description: Poly (ADP-ribose) polymerase (PARP), a nuclear enzyme involved in DNA repair, is a well known substrate for caspase-3 cleavage during apoptosis. Anti-PARP p85 Fragment pAb is a rabbit polyclonal antibody specific for the p85 fragment of PARP that results from caspase cleavage of the 116kDa intact molecule and thus provides an in situ marker for apoptosis. The antibody is affinity-purified using a peptide that corresponds to a region of the p85 fragment of PARP. The PARP immunogen is a synthetic peptide, gly-val-asp-glu-val-ala-lys (GVDEVAK), representing the N-terminus of the large C-terminal fragment of human PARP that results from caspase-3 cleavage. Each batch of antibody is quality assurance tested for use in immunostaining applications and contains sufficient antibody for 50 immunocytochemical reactions at the suggested working dilution of 1:100. Features: • Immunogen: N-terminal peptide from p85 fragment. • Antibody Form: Affinity-purified rabbit polyclonal antibody provided in Dulbecco’s PBS. • Specificity: Specifically detects PARP p85 fragment in human, rat and bovine cells and tissues. Does not recognize the 116kDa intact PARP protein. Storage Conditions: Store at –20°C. Protocol Anti-PARP p85 Fragment pAb Technical Bulletin Part# TB273 Size Cat.# Price (Fr) 50 µl G7341 709.00 Anti-Human p75 pAb Product Anti-Human p75 pAb For Research Use Only. Not for Use in Diagnostic Procedures. Description: The p75 neurotrophin receptor (p75NTR), also known as lowaffinity NGF receptor (LNGFR) and p75LNGFR, binds nerve growth factor, brainderived neurotrophic factor, neurotrophin-3 and neurotrophin-4 with varying specificities. p75NTR plays an important role in neurotrophic factor signaling including neuronal apoptosis. Anti-Human p75 pAb provides a valuable tool for understanding the role of p75NTR in neuronal death. Features: • Immunogen: Cytoplasmic domain of the human p75 neurotrophin receptor. • Antibody Form: Purified rabbit IgG; 1mg/ml in PBS containing 50μg/ml gentamicin. • Specificity: Human, rat, mouse and chicken p75. Storage Conditions: Store at 4°C. Size Cat.# Price (Fr) 200 µg G3231 790.00 8 Electron micrograph demonstrating immunostaining with AntiHuman p75 pAb in the inner molecular layer of the rat dentate gyrus. An axon terminal containing p75 immunoreactivity (A➤) is seen forming a synapse with a large unlabeled dendrite. Also labeled are a lengthwise axonal profile (B➤) and a small axonal cross section (C➤). Pre-embedding (Epon) immunohistochemistry was visualized with VECTASTAIN® ABC Reagent. Anti-PARP p85 Fragment pAb and TUNEL double-labeling of apoptotic Jurkat cells. Cells were labeled with the Anti-PARP p85 Fragment pAb (red) and the DeadEnd™ Fluorometric TUNEL System (Cat.# G3250; green). The colocalization of cleaved PARP in cells containing TUNEL-positive nuclei demonstrates that the Anti-PARP p85 Fragment pAb specifically labels apoptotic cells. Protocols developed and performed at Promega. Myelin sheath appears black due to OsO4 fixation. Image kindly provided by Drs. Karen Dougherty and Teresa Milner, Cornell University Medical College. For complete and up-to-date product information visit: www.promega.com/catalog 173 Imaging and Immunological Detection 2734TA08_9A 2029TA01_8A Pagina 176
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