Science Catalog 2012 Life Cell Signaling Anti-TGFβ1 pAb Product Anti-TGFβ1 pAb For Research Use Only. Not for Use in Diagnostic Procedures. Description: Transforming growth factor β1 (TGFβ1) is a 25kDa homodimer composed of two 12.5kDa subunits held together by disulfide bonds. TGFβ1 is a protein of immense interest to a number of fields and has been associated with intracellular matrix deposition and tissue repair/damage, cell cycle control and apoptosis. The Anti-TGFβ1 pAb is directed against biologically active human TGFβ1, providing a useful tool to analyze TGFβ1 in Western blot analysis or immunostaining applications. Features: • Immunogen: Biologically active human TGFβ1. • Antibody Form: Rabbit IgG provided at 1mg/ml in PBS containing 0.02mg/ml gentamicin as a preservative. • Specificity: Reacts with biologically active TGFβ1 with no cross-reactivity to TGFβ2 and TGFβ3. Storage Conditions: Store at –20°C. Anti-TrkB In pAb Product Anti-TrkB In pAb For Research Use Only. Not for Use in Diagnostic Procedures. Description: TrkB is the high-affinity receptor for brain-derived neurotrophic factor (BDNF) and neurotrophin-4 (NT-4). Two forms of the TrkB receptor exist: the full-length, active 145kDa protein and a truncated, nonsignaling 95kDa protein. The truncated isoform lacks the cytoplasmic tyrosine kinase catalytic region and therefore does not signal in response to BDNF or NT-4. The Anti-TrkB In pAb is a polyclonal antibody raised in chickens against a cytoplasmic domain of the carboxy terminus of mouse TrkB. Features: • Immunogen: A fusion protein containing residues 482–501 (HISNGSNTPSSSEGGPDAVI) derived from a portion of the intracellular domain of mouse TrkB generated in E. coli using a proprietary synthetic carrier protein technology. • Antibody Form: Chicken IgY provided at 0.5mg/ml in 0.1M NaCl, 0.01M K2PO4 (pH 7.4). • Specificity: Detects full-length TrkB, the high-affinity receptor for BDNF and NT-4, in human, rat and primate brain. Storage Conditions: Store at –20°C. Immunostaining for βIII tubulin in rat cerebellum using Anti-βIII Tubulin mAb. Paraffin-embedded rat brain section doubleimmunofluorescence labeled with the primary antibody and detected using an anti-mouse Cy®3-conjugated secondary antibody (yellow). Nuclei were stained with DAPI (blue). Protocols developed and performed at Promega. Size Cat.# Price (Fr) 100 µg G1561 402.00 Size Cat.# Price (Fr) 100 µg G1221 621.00 Anti-βIII Tubulin mAb Product Anti-βIII Tubulin mAb For Research Use Only. Not for Use in Diagnostic Procedures. Description: Anti-βIII Tubulin mAb is a protein G-purified IgG1 monoclonal antibody (from clone 5G8) raised in mice against a peptide (EAQGPK) corresponding to the C-terminus of βIII tubulin. It is directed against βIII tubulin, a specific marker for neurons. The major use of this antibody is for labeling neurons in tissue sections and cell culture. The antibody has been tested to perform in frozen and paraffin-embedded sections of rat brain, cerebellum and spinal cord, human and rat fetal CNS progenitor cell cultures and adult human paraffin-embedded brain. Features: • Immunogen: Peptide corresponding to the C-terminus (EAQGPK) of βIII tubulin. • Antibody Form: Mouse monoclonal IgG1 (clone 5G8), 1mg/ml in PBS containing no preservatives. • Specificity: Cross-reacts with most mammalian species. Does not label nonneuronal cells (e.g., astrocytes). Storage Conditions: Store at 4°C. Size Cat.# Price (Fr) 100 µg G7121 594.00 Immunohistochemical staining of rat cerebellum Purkinje cells with chicken Anti-TrkB In pAb. Visualized with a donkey anti-chicken IgY, biotin conjugate, followed by VECTASTAIN® Elite AB reagent, DAB reaction and OsO4 enhancement. Protocols developed and performed at Promega. 174 For complete and up-to-date product information visit: www.promega.com/catalog 1865TA08_7A 2137TA Pagina 177
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