Cell Signaling Anti-VAChT pAb Product Anti-VAChT pAb For Research Use Only. Not for Use in Diagnostic Procedures. Description: The purified Anti-VAChT (Vesicular Acetylcholine Transporter) pAb is raised in goats against a peptide (CSPPGPFDGCEDDYNYYSRS) corresponding to amino acids 511–530 of the carboxy terminus of the cloned rat VAChT. It is a novel tool to identify functional cholinergic neurons in the central and peripheral nervous system where the antibody stains fibers and neuronal cell bodies. This antibody has application for the study of the pathophysiology of neurodegenerative diseases involving the cholinergic system and for mapping cholinergic neurons in the nervous system. Features: • Immunogen: Carboxy-terminal peptide sequence 511–530 corresponding to cloned rat VAChT protein. • Antibody Form: Purified goat polyclonal IgG; 0.5mg/ml in PBS containing no preservatives. • Specificity: Cross-reacts with VAChT in rat and mouse, but not in human, guinea pig, rabbit or cat. Storage Conditions: Store at –20°C. Product Alkaline Phosphatase-Conjugated Antibodies Size Cat.# Price (Fr) Anti-Mouse IgG (H+L), AP Conjugate Anti-Rabbit IgG (Fc), AP Conjugate Anti-Human IgG (H+L), AP Conjugate Anti-Rat IgG (H+L), AP Conjugate Donkey Anti-Goat IgG, AP For Laboratory Use. 100 µl S3721 175.00 100 µl S3731 175.00 100 µl S3821 175.00 100 µl S3831 175.00 60 µl V1151 180.00 Immunofluorescence staining for VAChT protein in ventral gray matter of rat spinal cord. VAChT was detected using Anti-VAChT pAb at 1μg/ml followed by anti-goat Cy®3 conjugate. Specificity was demonstrated by preabsorption of Anti-VAChT pAb (1μg/ml) with the peptide immunogen (5μg/ml) prior to incubation with the primary antibody and anti-goat Cy®3 conjugate (data not shown). Protocols developed and performed at Promega. Donkey Anti-Rabbit IgG (H+L) HRP, AntiACTIVE® Qualified Product Donkey Anti-Rabbit IgG (H+L), HRP For Laboratory Use. Size Cat.# Price (Fr) 60 µl V7951 197.00 Description: Donkey Anti-Rabbit Ig, (H+L), HRP, are horseradish peroxidase-conjugated secondary antibodies specifically tested for use with the Anti-ACTIVE® antibodies. They are qualified for use in Western blot analysis using chemiluminescent and colorimetric detection methods. These antibody conjugates exhibit minimal cross-reactivity to goat, mouse and sheep IgG, bovine serum albumin (BSA) and proteins in mammalian cell extracts. These secondary antibody conjugates provide low background and highly specific signals when used at the recommended dilutions with Anti-ACTIVE® MAPK, Anti-ACTIVE® JNK and Anti-ACTIVE® p38 pAbs. The conjugates are provided in phosphate-buffered saline containing BSA as a stabilizer and gentamicin as a preservative. Description: The Alkaline Phosphatase-Conjugated Antibodies are used in the detection of proteins in Western blotting and ELISAs. The alkaline phosphatase (AP) catalyzes colorimetric reactions using Western Blue® Substrate (standard BCIP/NBT) or AttoPhos® Reagent. It can also drive the chemiluminescent detection reactions involving substrates such as 3-(2´-spiroadamantane)4-methyl-4-(3´´ phosphoryloxyphenyl-1, 2-dioxetane (AMPPD®). Goat Anti-Mouse IgG (H+L), Alkaline Phosphatase Conjugate is an affinity-purified goat anti-mouse antibody. It reacts with mouse IgG (all subclasses) as well as with light chains on other mouse immunoglobulins. Antibodies may cross-react with immunoglobulins from other species. Goat Anti-Rabbit IgG (Fc), Alkaline Phosphatase Conjugate is an affinitypurified goat anti-rabbit antibody. It reacts with the heavy chains of rabbit IgG but not with light chains. Antibodies may cross-react with immunoglobulins from other species. Goat Anti-Human IgG (H+L), Alkaline Phosphatase Conjugate is an affinity-purified goat anti-human antibody. This product has been isolated from antisera by immunoaffinity chromatography using immobilized antigens. It reacts with all subclasses of human IgG as well as with light chains on other human immunoglobulins. Antibodies may cross-react with immunoglobulins from other species. This product displays minimal cross-reactivity with horse and bovine serum proteins. Goat Anti-Rat IgG (H+L), Alkaline Phosphatase Conjugate is an affinitypurified goat anti-rat antibody. This product has been isolated from antisera by immunoaffinity chromatography using immobilized antigens. It reacts with heavy and light chains of rat IgG. Antibodies may cross-react with immunoglobulins from other species. Donkey Anti-Goat IgG, Alkaline Phosphatase Conjugate is a secondary antibody developed in donkeys against goat IgG; it has been affinity-purified and conjugated to alkaline phosphatase. Size Cat.# Price (Fr) 100 µg G4481 393.00 Features: • Sensitivity: When conjugates are used in a Western blot at a 1:10,000 dilution along with Anti-ACTIVE® MAPK pAb (Cat.# V8031), they can detect active MAP kinase in 5μg of activated (nerve growth factor [NGF]-treated) PC12 cell extract using colorimetric detection. • Specificity: Preferentially detects rabbit IgG with minimum reactivity with immunoglobulins from other species (including goat, sheep and mouse) or with bovine serum albumin and mammalian cell extract proteins. • Value: 60μl per vial, sufficient to generate 300–600ml of Western blotting solution when used at the recommended dilution of 1:5,000 to 1:10,000. • Immunogen: Intact rabbit IgG (H+L chains). • Antibody Form: Donkey IgG, affinity-purified polyclonal antibody conjugated to horseradish peroxidase (HRP). Storage Conditions: Store at –20°C. Protocol Anti-ACTIVE® MAPK, JNK and p38 Polyclonal Antibodies and Anti-ACTIVE® Qualified Secondary Antibody Conjugates Technical Bulletin Anti-ACTIVE® CaM KII pAb, (pT286) and Anti-ACTIVE® Qualified Secondary Antibody Conjugates Technical Bulletin Part# TB262 TB264 8 For complete and up-to-date product information visit: www.promega.com/catalog 175 Imaging and Immunological Detection 2018TB Pagina 178
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