Science Catalog 2012 Life Cell Signaling Horseradish Peroxidase-Conjugated Antibodies Product Anti-Rabbit IgG (H+L), HRP Conjugate Anti-Mouse IgG (H+L), HRP Conjugate Anti-Human IgG (H+L), HRP Conjugate Anti-Chicken IgY, HRP Conjugate Donkey Anti-Goat IgG, HRP Size Cat.# Price (Fr) 300 µl W4011 205.00 300 µl W4021 205.00 300 µl W4031 205.00 300 µl G1351 217.00 60 µl V8051 197.00 W4011, W4021, W4031, V8051 For Laboratory Use. G1351 For Research Use Only. Not for Use in Diagnostic Procedures. Description: These high-quality antibodies are raised in goat, rabbit or donkey, affinity-purified and conjugated to horseradish peroxidase. The human, mouse and rabbit antibodies react with all IgG subclasses. All antibodies may crossreact with immunoglobulins of other species. Goat Anti-Rabbit IgG (H+L), HRP Conjugate is an affinity-purified goat anti-rabbit antibody. This antibody has been isolated from antisera by immunoaffinity chromatography using immobilized antigens. This anti-rabbit antibody reacts with heavy and light chains of rabbit IgG. A working dilution of 1:2,500 is suggested for Western blots and ELISAs. Goat Anti-Mouse IgG (H+L), HRP Conjugate is an affinity-purified goat anti-mouse antibody. This antibody has been isolated from antisera by immunoaffinity chromatography using immobilized antigens. This anti-mouse antibody reacts with all subclasses of mouse IgG as well as with light chains on other mouse immunoglobulins. A working dilution of 1:2,500 is suggested for Western blots and ELISAs. Goat Anti-Human IgG (H+L), HRP Conjugate is an affinity-purified goat anti-human antibody. This product has been isolated from antisera by immunoaffinity chromatography using immobilized antigens. This anti-human antibody reacts with all subclasses of human IgG as well as with light chains on other human immunoglobulins. A working dilution of 1:2,500 is suggested for Western blots and ELISAs. Rabbit Anti-Chicken IgY, HRP Conjugate is a secondary antibody developed in rabbits against chicken IgY; it has been affinity-purified and conjugated to horseradish peroxidase. Anti-Chicken IgY, HRP Conjugate recognizes both the heavy and light chains of IgY and has been validated for use in Western blots, dot blots and ELISAs. Donkey Anti-Goat IgG, HRP Conjugate is a secondary antibody developed in donkeys against goat IgG; it has been affinity-purified and conjugated to horseradish peroxidase. When used in a Western blot at a 1:10,000 dilution with colorimetric detection, Donkey Anti-Goat IgG, HRP Conjugate shows reactivity to goat and sheep IgG but minimal cross-reactivity to rabbit and mouse IgG and extracts of PC12 cells. It detects 100ng of goat IgG with minimal background signal. Anti-Chicken IgY, HRP Conjugate Product Anti-Chicken IgY, HRP Conjugate For Research Use Only. Not for Use in Diagnostic Procedures. Description: This secondary antibody is developed in rabbits against chicken IgY, and has been affinity purified and conjugated to horseradish peroxidase. The Anti-Chicken IgY, HRP Conjugate recognizes both the heavy and light chains of IgY. This antibody has been validated for use in Western blots, dot blots and ELISAs. Formulation: 1mg/ml in 10mM KPO4 (pH 7.6), 0.15M NaCl, 10mg/ml BSA and 0.01% gentamicin. Storage Conditions: Store at –20°C. Avoid multiple freeze-thaw cycles. When stored and handled properly, this product is stable for 6 months from date of purchase. Size Cat.# Price (Fr) 300 µl G1351 217.00 Chicken IgY, Control Immunoglobulin Product Chicken IgY, Control Immunoglobulin For Laboratory Use. Size Cat.# Price (Fr) 1 mg G1161 191.00 Description: Chicken IgY, Control Immunoglobulin is specific to chickens and is the counterpart to IgG from mammals. Chicken IgY may be used as a control antigen when performing Westerns, ELISAs and in vivo and in vitro immunohistochemical studies using anti-chicken IgY antibodies. The antibody is prepared by sequential precipitation, is chromatography-purified and is provided at 1mg/ml in PBS with no preservatives. The purity is >75%. TMB One Solution Product TMB One Solution For Laboratory Use. Size Cat.# Price (Fr) 100 ml G7431 139.00 Description: TMB One Solution is a chromagen substrate, 3,3´,5,5´tetramethylbenzidine (TMB) provided in a mildly acidic, nonhazardous buffer for horseradish peroxidase detection in an ELISA format. The substrate is provided as a single solution at a ready-to-use working dilution. The substrate develops a blue reaction product when oxidized by peroxidase and a yellow reaction product in an endpoint multiwell assay after the addition of an acid solution provided by the end user. Features: • Convenient: Single solution provided ready-to-use; just add, incubate, stop and read. This homogeneous reagent improves assay variation. • Stable: Stable for 12 months at 4°C, providing extended shelf life; the assay end product is stable for at least one hour after stopping the assay. • Safe: Provided in a slightly acidic, nonhazardous proprietary buffer without aprotic solvents; noncaustic to plastics used in automated systems. • Sensitive: Low background provides greater assay sensitivity. Storage Conditions: Store at 4°C protected from light. In vivo Imaging VivoGlo™ Luciferin, In Vivo Grade Product VivoGlo™ Luciferin, In Vivo Grade For Research Use Only. Not for Use in Diagnostic Procedures. Description: Luciferase genes from the North American firefly (Photinus pyralis) and from other beetles are commonly used as light-emitting reporters in cellular and animal models. VivoGlo™ Luciferin is the potassium salt of D-luciferin, the firefly luciferase substrate capable of generating light when a suitable model is used. VivoGlo™ In Vivo Imaging Substrates are provided in cooperation with Xenogen Corporation and Caliper Life Sciences for use in in vivo bioluminescence imaging applications. Features: • Highest Quality Substrates: Eliminate potential interference in assays due to the presence of endotoxins. • Assured Product Integrity: Most products are packaged in amber vials with septa to ensure product integrity as well as offer ease of dilution and use for imaging experiments. Product is packaged with fine tolerances to minimize the need to weigh substrates. • Flexibility and Convenience: Available in multiple sizes to accommodate a variety of experimental settings. Storage Conditions: Store at –20°C. 176 For complete and up-to-date product information visit: www.promega.com/catalog Size Cat.# Price (Fr) 50 mg P1041 480.00 250 mg P1042 960.00 1 g P1043 Pls. Enq. Pagina 179
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