Science Catalog 2012 Life Cell Signaling Hot-Start PCR GoTaq® Hot Start Polymerase Product GoTaq® Hot Start Polymerase 100 u M5001 93.00 500 u M5005 338.00 2,500 u M5006 1521.00 10,000 u M5008 Pls. Enq. GoTaq® Hot Start Green Master Mix GoTaq® Hot Start Colorless Master Mix 100 reactions M5122 164.00 1,000 reactions M5123 1230.00 100 reactions M5132 164.00 1,000 reactions M5133 1230.00 For Research Use Only. Not for Use in Diagnostic Procedures. a tube of 25mM MgCl2, allowing optimization of the magnesium concentration in your reactions. It is also supplied with 5X Green GoTaq® Flexi Buffer and 5X Colorless GoTaq® Flexi Buffer. The buffers contain a compound that increases sample density, so that samples sink easily into the wells of an agarose gel. The green buffer also contains two dyes (yellow and blue) that separate to allow easy monitoring during electrophoresis. Use the green reaction buffer for direct-to-gel analysis after amplification and the colorless reaction buffer for amplifications where the dyes may interfere with postamplification analysis such as fluorescence or absorbance testing. Description: GoTaq® Hot Start Polymerase contains the high-performance GoTaq® DNA Polymerase bound to a proprietary antibody that blocks polymerase activity. The polymerase activity is restored during the initial denaturation step when the amplification reactions are heated at 94–95°C for two minutes. This allows for hot-start PCR, where polymerase activity is eliminated or minimized at temperatures below 70°C. GoTaq® Hot Start Polymerase exhibits 5´→3´ exonuclease activity. The system is supplied with GoTaq® Hot Start Master Mixes are premixed, ready-to-use solutions containing GoTaq® Hot Start Polymerase, magnesium, dNTPs and buffer. Reactions can be set up in less than a minute at room temperature; simply add your template, water and primers. Available with either green or colorless reaction buffers, which also serve as loading buffers, allowing you to go directly from thermal cycler to gel analysis. GoTaq® Hot Start Master Mixes offer the specificity and sensitivity of an antibody-based hot-start polymerase in a convenient, easy-to-use, time-saving format. Features: • Enhance Yield, Sensitivity and Specificity: The proven, robust amplification and sensitivity of GoTaq® DNA Polymerase now with a built-in hot start to deliver even more superior results. • Ease of Use: Set up your reaction at room temperature—no need to set up on ice. • Higher Yield: Two-minute activation saves time and ensures maximum enzyme activity. • Higher Specificity: Minimize nonspecific amplification and primer-dimers. • Improve Productivity: Go directly from PCR to gel analysis. Green GoTaq® Reaction Buffer serves as both reaction buffer and gel-loading solution. • Convenient: One tube, one pipetting step. Only add template and primers when using the master mixes. • Optimization: Control the magnesium in your reaction for specialized templates when using the standalone polymerase. Storage Conditions: Store at –20°C. Protocol GoTaq® Hot Start Polymerase Protocol GoTaq® Hot Start Green Master Mix Protocol GoTaq® Hot Start Colorless Master Mix Protocol Part# 9PIM500 9PIM512 9PIM513 517 350 bp 1,605 1,198 Standard Taq GoTaq® Hot Start Size Cat.# Price (Fr) MM Improve amplification of targets that require hot start using GoTaq® Hot Start Polymerase. A 1.5kb fragment of a Corynephage omega gene that requires hot start PCR was amplified from 500pg of plasmid DNA using either standard Taq or GoTaq® Hot Start Polymerase in Green and Colorless Flexi Reaction Buffers. Use of GoTaq® Hot Start Polymerase resulted in amplification of only the target 1.5kb fragment. Using standard Taq DNA Polymerase, a nonspecific 410bp product also was amplified. 206 For complete and up-to-date product information visit: www.promega.com/catalog 6988TA Colorless Green Colorless Green Pagina 209
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