Cell Signaling TaqBead™ Hot Start Polymerase Product TaqBead™ Hot Start Polymerase, 1.25u/ bead, Nonbarrier For Research Use Only. Not for Use in Diagnostic Procedures. Description: TaqBead™ Hot Start Polymerase consists of spherical beads of wax containing Taq DNA polymerase. TaqBead™ Hot Start Polymerase facilitates hot-start PCR by keeping the enzyme sequestered in paraffin wax until the reaction temperature reaches approximately 60°C. This increases PCR specificity by keeping the polymerase separate from the rest of the reaction components until a critical temperature is reached, decreasing the probability of amplifying products that are the result of nonspecific binding of primers. The nonbarrier format of the bead is intended for use with heated-lid thermal cyclers or with the addition of a mineral oil overlay. Thermophilic DNA Polymerase 10X Reaction Buffer: 500mM KCl, 100mM Tris-HCl (pH 9.0) at 25°C, 1.0% Triton® X-100. Magnesium Chloride: 25mM MgCl2 Solution included. Features: • Higher Yield PCR: Hot-start PCR protocols increase amplimer yield by minimizing nonspecific priming, primer-dimer formation or other reactions that can occur at low temperatures once all the PCR amplification components are mixed. • Increased Specificity: Hot-start format reduces the incidence of nonspecific amplification products. • Reliable: Minimized nonspecific priming results in increased reproducibility of amplification reactions. • Flexible: Sufficient 25mM MgCl2 is provided separately to allow optimization of enzyme performance under different conditions. Storage Conditions: Store at –20°C. Protocol TaqBead™ Hot Start Polymerase Technical Bulletin Part# TB247 Size Cat.# Price (Fr) 100 reactions M5661 196.00 Long PCR GoTaq® Long PCR Master Mix Product GoTaq® Long PCR Master Mix For Research Use Only. Not for Use in Diagnostic Procedures. Description: GoTaq® Long PCR Master Mix contains the high-performance GoTaq® Hot Start Polymerase in a specially formulated mixture with a proprietary thermal stable proofreading polymerase. This optimized enzyme mixture allows efficient amplification of up to 40kb from lambda DNA or 30kb from human genomic DNA. The presence of a proofreading enzyme to repair DNA mismatches in the presence of a highly processive polymerase allows the polymerase to continue to elongate the DNA much further, resulting in longer DNA amplification. The optimized formulation of the GoTaq® Long PCR Master Mix components have been engineered to enable simple reaction setup and provide consistently efficient, accurate and robust performance of long DNA amplicons. Features: The proven robust amplification of GoTaq® Polymerase is now available for long-range PCR (up to 30kb gDNA). • Easy: Hot-start master mix for convenient handling and simple setup. • Enhanced: Yield, sensitivity and specificity with optimized components. • Accurate: Blend of thermostable DNA polymerases with enhanced processivity and proofreading. • Confidence: Control primer pair and human gDNA template to control reactions and test template quality. • Efficient: Perfect for cloning genes, mutational analysis and DNA sequencing. Storage Conditions: Upon arrival, store all components at –20°C, protected from light. For immediate use, components may be stored at 2–8°C, protected from light, for up to 3 months. Protocol GoTaq® Long PCR Master Mix Technical Manual Part# TM359 Size Cat.# Price (Fr) 100 reactions M4021 Pls. Enq. 12 For complete and up-to-date product information visit: www.promega.com/catalog 207 PCR Pagina 210
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