Science Catalog 2012 Life Cell Signaling Tfl DNA Polymerase Product Tfl DNA Polymerase For Laboratory Use. Description: Tfl DNA Polymerase is a thermostable enzyme of approximately 94kDa isolated from Thermus flavus. The enzyme replicates DNA at 74°C and exhibits a half-life of 40 minutes at 95°C. Tfl DNA Polymerase catalyzes the polymerization of nucleotides into duplex DNA in the 5´→3´ direction in the presence of magnesium and the polymerization of nucleotides into DNA using an RNA template in the 5´→3´ direction in the presence of manganese. The enzyme also possesses a 5´→3´ exonuclease activity. Tfl DNA Polymerase is recommended for use in PCR and primer extension reactions at elevated temperatures. Tfl DNA Polymerase 10X Reaction Buffer: 200mM Tris-acetate (pH 8.9 at 25°C), 100mM ammonium sulfate. Magnesium Sulfate: 25mM MgSO4 Solution included. Features: • Flexible: Provided with a 10X Reaction Buffer that does not contain magnesium. Sufficient 25mM MgSO4 is provided separately to allow optimization of enzyme performance under different conditions. • Performance Guarantee: Promega PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any Promega PCR product, we will send a replacement or refund your account. Storage Conditions: Store at –20°C. Pfu DNA Polymerase Product Pfu DNA Polymerase Size Conc. Cat.# Price (Fr) 100 u 2–3 u/µl M7741 162.00 500 u 2–3 u/µl M7745 628.00 For Research Use Only. Not for Use in Diagnostic Procedures. Product may not be available in all countries. Please contact your local representative for more information. Description: Pfu DNA Polymerase is a thermostable enzyme of approximately 90kDa isolated from Pyrococcus furiosus. The enzyme replicates DNA at 75°C, catalyzing the polymerization of nucleotides into duplex DNA in the 5´→3´ direction in the presence of magnesium. Pfu DNA Polymerase also possesses 3´→5´ exonuclease (proofreading) activity. Base misinsertions that may occur during polymerization are rapidly excised by the proofreading activity of the polymerase. Consequently, Pfu DNA Polymerase is recommended for use in PCR and primer extension reactions that require high-fidelity synthesis. Pfu DNA Polymerase-generated PCR fragments are blunt-ended. Pfu DNA Polymerase 10X Reaction Buffer with MgSO4: 200mM Tris-HCl (pH 8.8 at 25°C), 100mM KCl, 100mM (NH4)2SO4, 20mM MgSO4, 1.0% Triton® X-100 and 1mg/ml nuclease-free BSA. Features: • High Fidelity: Pfu DNA Polymerase exhibits the lowest error rate of any thermostable DNA polymerase. • Complete: Provided with 10X Buffer containing 20mM MgSO4. • Performance Guarantee: Promega PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any Promega PCR product, we will send a replacement or refund your account. Storage Conditions: Store at –20°C. Protocol Pfu DNA Polymerase Protocol Part# 9PIM774 dNTP Mix Product dNTP Mix For Laboratory Use. Description: dNTP Mix is a premixed solution containing sodium salts of dATP, dCTP, dGTP and dTTP, each at 10mM in water; the total concentration of nucleotides, therefore, is 40mM (pH 7.5). One microliter of the dNTP Mix into a 50μl reaction will give a final dNTP concentration of 200μM for each dNTP. Features: • High Purity: dNTPs are >98% pure. • Easy to Use: Reduced pipetting steps contribute to ease-of-use and reduce the risk of contamination. Storage Conditions: Store at –20°C. Avoid exposure to frequent temperature changes. Size Conc. Cat.# Price (Fr) 200 µl 10 mM U1511 44.00 1,000 µl 10 mM U1515 187.00 Size Conc. Cat.# Price (Fr) 100 u 5 u/µl M1941 84.00 1,000 u 5 u/µl M1945 672.00 PCR Nucleotide Mix Product PCR Nucleotide Mix For Laboratory Use. Description: High-quality deoxynucleotide triphosphates (dNTPs) are critical for the success of the polymerase chain reaction (PCR). The PCR Nucleotide Mix is a premixed solution containing the sodium salts of dATP, dCTP, dGTP and dTTP, each at a concentration of 10mM in water; the total concentration of nucleotides, therefore, is 40mM (pH 7.5). This solution is ready to use and is optimized for standard polymerase chain reactions and specialty approaches including hot-start and reverse transcription PCR (RT-PCR). One microliter (1μl) is sufficient for PCR amplification in a typical 50μl reaction volume. Features: • Optimized and Pretested in PCR: Equimolar amounts of each dNTP ensure optimal PCR. • Convenient: 1μl addition for 50μl PCR. • Easy to Use: Reduced pipetting steps contribute to ease-of-use and reduce the risk of contamination. • Performance Guarantee: Promega PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any Promega PCR product, we will send a replacement or refund your account. • Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/myway/ Storage Conditions: Store at –20°C. Avoid exposure to frequent temperature changes. Protocol PCR Nucleotide Mix Protocol Part# 9PIC114 Size Conc. Cat.# Price (Fr) 200 µl 10 mM C1141 124.00 1,000 µl 10 mM C1145 450.00 210 For complete and up-to-date product information visit: www.promega.com/catalog Pagina 213
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