Cell Signaling GoTaq® PCR Core Systems Product GoTaq® PCR Core System I GoTaq® PCR Core System II For Research Use Only. Not for Use in Diagnostic Procedures. Description: The GoTaq® PCR Core Systems I and II are designed for the exponential amplification of specific regions of DNA using the polymerase chain reaction. Both systems include GoTaq® DNA polymerase and PCR Nucleotide Mix, along with high-performance buffers and magnesium chloride. The GoTaq® PCR Core System II also includes a Positive Control Plasmid DNA and Positive Control Primers, providing increased confidence in PCR control reactions. Each system’s components are performance-tested in PCR and are sufficient for 200 reactions. The GoTaq® PCR Core Systems are provided with a Technical Bulletin that provides simple protocols and a complete troubleshooting guide. Features: • Convenient: PCR-tested components are provided in optimized volumes for 200 reactions. • Flexible: Optimization tools are provided for reaction flexibility. • Positive Controls: The GoTaq® PCR Core System II provides Positive Control Plasmid DNA and Control Primers to help troubleshoot PCR parameters. • Performance Guarantee: Promega PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any Promega PCR product, we will send a replacement or refund your account. Storage Conditions: Store all components at –20°C. Protocol GoTaq® PCR Core Systems Technical Bulletin aTaq DNA Polymerase Product aTaq DNA Polymerase Size Conc. Cat.# Price (Fr) 100 u 5 u/µl M1241 64.00 500 u 5 u/µl M1245 232.00 2,500 u 5 u/µl M1248 1044.00 For Research Use Only. Not for Use in Diagnostic Procedures. Description: aTaq DNA Polymerase contains a nonrecombinant, modified form of Taq DNA polymerase that lacks 5´→3´ exonuclease activity. The enzyme is supplied in a proprietary formulation containing 50% glycerol. It is supplied with Thermophilic DNA Polymerase 10X Reaction Buffer, Mg-Free, and Magnesium Chloride Solution to allow the optimization of magnesium concentration. Features: • Reliable: Specifically formulated for routine PCR. • Flexible: Allows optimization of Mg2+ for each template. • Performance Guarantee: Promega PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any Promega PCR product, we will send a replacement or refund your account. Storage Conditions: Store at –20°C. Protocol aTaq DNA Polymerase Protocol Part# 9PIM124 Detection of low-copy-number templates using PCR Master Mix. Use of PCR Master Mix to detect low number of copies of the α-1-antitrypsin gene. PCR was performed on Human Genomic DNA (Cat.# G3041) using primers targeting a 360bp fragment of the α-1-antitrypsin gene (single copy per genome). Lane M, 100bp DNA Ladder (Cat.# G2101). Part# TB254 For Laboratory Use. Description: PCR Master Mix is a premixed, ready-to-use solution containing Taq DNA polymerase, dNTPs, MgCl2 and reaction buffers at optimal concentrations for efficient amplification of DNA templates by PCR. The PCR Master Mix has been optimized for use in routine PCR for amplifying DNA templates in the range of 0.2–2kb. Features: • Fast: Set up reactions in less than a minute. • Sensitive: Amplify as little as 2 copies of target template. • Convenient: One tube, one pipetting step. • Complete: Reagents, including Taq DNA polymerase, MgCl2, dNTPs and buffers, in one tube. • Scalable: Set up 10μl, 25μl or 50μl reactions. • Stable: Store at 4°C for up to 3 months. • Performance Guarantee: Promega PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any Promega PCR product, we will send a replacement or refund your account. • Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/myway/ Storage Conditions: Store at –20°C. PCR Master Mix can be stored at 4°C for up to 3 months. Protocol PCR Master Mix Protocol MM100 Template Copies per Reaction 0 2 10 1,000 Part# 9PIM750 Size Cat.# Price (Fr) 200 reactions M7650 225.00 200 reactions M7655 248.00 PCR Master Mix Product PCR Master Mix Size Conc. Cat.# Price (Fr) 10 reactions 2 X M7501 27.00 100 reactions 2 X M7502 162.00 1,000 reactions 2 X M7505 1215.00 12 For complete and up-to-date product information visit: www.promega.com/catalog 209 PCR 3063TA09_0A Pagina 212
Pagina 214Interactieve digi handleiding, deze PDF of reclamefolder is levensecht online geplaatst met Online Touch en bied het bladerbaar maken van eonderwijscatalogi.
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