Science Catalog 2012 Life Cell Signaling Tth DNA Polymerase Product Tth DNA Polymerase Size Conc. Cat.# Price (Fr) 100 u 5 u/µl M2101 112.00 500 u 5 u/µl M2105 448.00 For Research Use Only. Not for Use in Diagnostic Procedures. Description: Tth DNA Polymerase is a thermostable enzyme of approximately 94kDa isolated from Thermus thermophilus HB-8. The enzyme replicates DNA at 74°C and exhibits a half-life of 20 minutes at 95°C. Tth DNA Polymerase catalyzes the polymerization of nucleotides into duplex DNA in the 5´→3´ direction in the presence of magnesium and the polymerization of nucleotides into DNA using an RNA template in the 5´→3´ direction in the presence of manganese. The enzyme also possesses a 5´→3´ exonuclease activity. Tth DNA Polymerase is recommended for use in PCR, RT-PCR, reverse transcription and primer extension reactions at elevated temperatures. 10X Reverse Transcription Buffer: 100mM Tris-HCl (pH 8.3 at 25°C), 900mM KCl. 10X Chelate Buffer: 100mM Tris-HCl (pH 8.3 at 25°C), 1M KCl, 7.5mM EGTA, 0.5% Tween® 20, 50% glycerol. Thermophilic DNA Polymerase 10X Reaction Buffer: 500mM KCl, 100mM Tris-HCl (pH 9.0 at 25°C) and 1% Triton® X-100. Buffer is optimized for use with 0.2mM of each of the dNTPs. Manganese and Magnesium Chloride: 10mM MnCl2 and 25mM MgCl2 Solutions provided. Features: • Increased Specificity for RT-PCR: The ability to reverse transcribe at higher temperatures results in increased specificity of primer hybridization and extension. • Minimized Secondary Structures: Higher temperature RT-PCR minimizes problems associated with strong secondary structures in RNA. • Performance Guarantee: Promega PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any Promega PCR product, we will send a replacement or refund your account. Storage Conditions: Store at –20°C. Protocol Tth DNA Polymerase Protocol Primer Pairs for RT-PCR Product β-Actin Primer Pair CNTF Primer Pair NT-3 Primer Pair p75 Primer Pair Size Cat.# Price (Fr) 20 reactions G5740 274.00 20 reactions G5770 274.00 20 reactions G6801 274.00 20 reactions G6861 274.00 For Research Use Only. Not for Use in Diagnostic Procedures. Description: Promega Primer Pairs for RT-PCR provide convenient primers for analyzing the expression of specific mRNAs by RT-PCR. Each Primer Pair includes a 5´ and 3´ oligonucleotide primer provided in individual vials at 100μM ready for addition to the amplification reaction. The Primer Pairs have been confirmed in two-step conventional RT-PCR (Reverse Transcription System, Cat.# A3500) using rat brain total RNA. They also have been tested in one-step RT-PCR (Access RT-PCR System, Cat.# A1260) with human and mouse brain total RNA. PCR products from the use of the Primer Pairs have been sequence-confirmed for appropriate identity. Storage Conditions: Store at –20°C. Part# 9PIM210 Oligonucleotides and Primers: cDNA Synthesis and Cloning Product Oligo(dT)15 Primer Random Primers EcoRI Adaptors Size Cat.# Price (Fr) 20 µg C1101 118.00 20 µg C1181 82.00 150 pmol C1291 69.00 C1181 For Laboratory Use. C1101, C1291 For Research Use Only. Not for Use in Diagnostic Procedures. Description: Oligo(dT)15 Primer is suitable for the use of a primer for firststrand cDNA synthesis with a reverse transcriptase. The primer hybridizes to the poly(A) tail of mRNA. Random Primers can be used for first-strand cDNA synthesis and cloning; they are also available as components of the Universal Riboclone® cDNA Synthesis System (Cat.# C4360) and the Reverse Transcription System (Cat.# A3500). The primers are random hexadeoxynucleotides. The EcoRI Adaptors consist of two complementary oligonucleotides: a 16mer and a 12mer phosphorylated at the 5´-end. The oligonucleotides are provided annealed in equimolar concentrations in water. The EcoRI Adaptors are designed to attach EcoRI “sticky” ends to blunt-ended DNA. Storage Conditions: Store at –20°C. Product Universal RiboClone® cDNA Synthesis System Size Cat.# Price (Fr) Universal RiboClone® cDNA Synthesis System Available Separately Oligo(dT)15 Primer Random Primers Spin Columns EcoRI Adaptors 1.2kb Kanamycin Positive Control RNA Sephacryl® S-400 1 system C4360 1037.00 20 µg C1101 118.00 20 µg C1181 82.00 10 each C1281 69.00 150 pmol C1291 69.00 5 µg C1381 121.00 10 ml V3181 82.00 C1181, C1381, V3181 For Laboratory Use. C1101, C4360, C1281, C1291 For Research Use Only. Not for Use in Diagnostic Procedures. Description: The Universal RiboClone® cDNA Synthesis System contains the reagents required for the synthesis of double-stranded cDNA from mRNA and subsequent ligation into a suitable vector. The system is based on the method described by Okayama and Berg with modifications by Gubler and Hoffman. First-strand synthesis is driven by AMV (Avian Myeloblastosis Virus) Reverse Transcriptase and either Random Primers or an Oligo(dT)15 Primer, followed directly by second-strand replacement synthesis using RNase H and DNA Polymerase I. After treatment with T4 DNA Polymerase to flush the ends, the double-stranded cDNA molecules are prepared for cloning by size fractionation and the addition of EcoRI Adaptors. The resulting cDNA preparation can then be cloned into a suitable vector. Features: • Convenient: Contains all the necessary reagents to synthesize doublestranded cDNA from RNA. • Flexible: Both Oligo(dT)15 Primer and Random Primers are included, providing the researcher a choice of priming methods. Storage Conditions: Store control RNA at –70°C. Store Sephacryl® S-400 at 4°C and Spin Columns at room temperature. Store other components at –20°C. Protocol Universal RiboClone® cDNA Synthesis System Technical Manual Part# TM038 220 For complete and up-to-date product information visit: www.promega.com/catalog Pagina 223
Pagina 225Interactieve digi-folder, deze clubblad of boek is levensecht online geplaatst met Online Touch en bied het online bladerbaar uitgeven van e-presentaties.
Promega Switzerland - Life Sciences Catalog 2012 Lees publicatie 100Home