Cell Signaling AMV Reverse Transcriptase Product AMV Reverse Transcriptase AMV Reverse Transcriptase (HC) For Laboratory Use. Size Conc. 300 u 10 u/µl 1,000 u 10 u/µl Cat.# Price (Fr) M5101 170.00 M5108 482.00 600 u 20–25 u/µl M9004 298.00 Description: Avian Myeloblastosis Virus Reverse Transcriptase (AMV RT) catalyzes the polymerization of DNA using template DNA, RNA or RNA:DNA hybrids. It requires a primer (DNA primers are more efficient than RNA primers) as well as Mg2+ or Mn2+. The enzyme possesses an intrinsic RNase H activity. Both nonionic detergents and sulfhydryl compounds stabilize the enzyme activity in vitro. Features: • Available at High Concentration: Cat.# M9004 contains 600 units of AMV RT at 20–25u/μl. • Provided with 5X Reaction Buffer: 250mM Tris-HCl (pH 8.3 at 25°C), 250mM KCl, 50mM MgCl2, 2.5mM spermidine, 50mM DTT. • Temperature Stability: AMV RT is the preferred reverse transcriptase for templates with high secondary structure due to its stability at higher reaction temperatures (37–58°C). Storage Conditions: Store at –20°C. Protocol AMV Reverse Transcriptase Protocol M-MLV Reverse Transcriptase Product M-MLV Reverse Transcriptase M-MLV Reverse Transcriptase Buffer Pack For Laboratory Use. Description: Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long messenger RNA templates (>5kb). The enzyme is a product of the pol gene of M-MLV and consists of a single subunit with a molecular weight of 71kDa. The RNase H activity of M-MLV RT is weaker than the commonly used Avian Myeloblastosis Virus (AMV) reverse transcriptase. Features: • Provided with 5X Reaction Buffer: 250mM Tris-HCl (pH 8.3 at 25°C), 375mM KCl, 15mM MgCl2, 50mM DTT. • May Be Heat-Inactivated: M-MLV RT is inactivated by heating at 70°C for 10 minutes. • Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/myway/ Storage Conditions: Store at –20°C. Protocol M-MLV Reverse Transcriptase Protocol Part# 9PIM170 Size Conc. Cat.# Price (Fr) 10,000 u 200 u/µl M1701 123.00 50,000 u 200 u/µl M1705 477.00 2 × 1 ml M5313 55.00 Part# 9PIM510 M-MLV Reverse Transcriptase, RNase H Minus Product M-MLV Reverse Transcriptase, RNase H Minus Size Conc. Cat.# Price (Fr) 10,000 u 100–200 u/µl M5301 296.00 For Research Use Only. Not for Use in Diagnostic Procedures. Product may not be available in all countries. Please contact your local representative for more information. Description: Moloney Murine Leukemia Virus Reverse Transcriptase, RNase H Minus (M-MLV RT [H–]), is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long messenger RNA templates (>5kb). This is a form of M-MLV Reverse Transcriptase that has been genetically altered to remove the associated RNase H activity. Although many researchers are successful in using M-MLV RT (H+) for analytical and some preparative cDNA applications, reverse transcriptases lacking RNase H activity provide another option for the preparation of long cDNAs and libraries containing a high percentage of full-length cDNA. Features: • RNase H Minus: Provides optimal conditions for the preparation of fulllength cDNA from long RNA templates. • Provided with 5X Reaction Buffer: 250mM Tris-HCl (pH 8.3 at 25°C), 375mM KCl, 15mM MgCl2, 50mM DTT. • May Be Heat-Inactivated: M-MLV RT is inactivated by heating at 70°C for 10 minutes. Storage Conditions: Store at –20°C. Protocol M-MLV Reverse Transcriptase, RNase H Minus Protocol M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant Product M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant Size Cat.# Price (Fr) 2,500 u M3681 94.00 10,000 u M3682 329.00 50,000 u M3683 1399.00 For Research Use Only. Not for Use in Diagnostic Procedures. Product may not be available in all countries. Please contact your local representative for more information. Description: Moloney Murine Leukemia Virus Reverse Transcriptase, RNase H Minus (M-MLV RT [H–]), Point Mutant, is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long RNA templates (>5kb). The lack of RNase H activity is beneficial for this application, as RNase H can start to degrade templates when incubation times are long, as they may be when making long cDNAs. Although many researchers are successful in using M-MLV RT (H+) for analytical and some preparative cDNA applications, reverse transcriptases lacking RNase H activity provide another option for the preparation of long cDNAs and for libraries containing a high percentage of full-length cDNA. Features: • RNase H Minus: Provides optimal conditions for the preparation of fulllength cDNA from long RNA templates. • Temperature Stability: Thermostability of this point mutant prevents problems associated with secondary structure. • Increased Polymerase Activity: M-MLV RT (H–), Point Mutant, gives higher yields of cDNA compared with the deletion mutant (Cat.# M5301). • Provided with 5X Reaction Buffer: 250mM Tris-HCl (pH 8.3 at 25°C), 375mM KCl, 15mM MgCl2, 50mM DTT. • Broad Working Range: More tolerance to variations in enzyme and substrate concentration means improved consistency in performance. Storage Conditions: Store at –20°C. Protocol Part# M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant Protocol 9PIM368 For complete and up-to-date product information visit: www.promega.com/catalog 219 Part# 9PIM530 12 PCR Pagina 222
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