Cell Signaling ProtoBlot® II AP Systems with Stabilized Substrate and Western Express® Fast Blotting Protocol Product ProtoBlot® II AP System with Stabilized Substrate, Human ProtoBlot® II AP System with Stabilized Substrate, Mouse ProtoBlot® II AP System with Stabilized Substrate, Rabbit For Laboratory Use. Description: The ProtoBlot® II AP Systems with Stabilized Substrate are designed for the rapid and sensitive detection of proteins or other macromolecular antigens immobilized on nitrocellulose or PVDF membranes. Proteins can be transferred from gels after electrophoresis (Western blots) or bound directly from solution (“dot” blots). The Western Express® Fast Blotting Protocol is included with the system and can reduce dramatically the time required to perform immunodetection. All ProtoBlot® II AP Systems contain BSA as a stabilizer and 0.05% sodium azide as a preservative. Features: • Fast: Easy-to-use Western Express® Protocol allows the detection of dot blots in 30–45 minutes and the detection of Western blots in 1–2 hours. • Convenient: The system contains Western Blue® Stabilized Substrate for AP, which is a ready-to-use solution of BCIP/NBT. No reagent preparation is required for the substrate. For many applications, AP conjugates are superior to HRP conjugates because they: • offer greater sensitivity (tenfold) of detection. • are not inhibited by azide. • use a substrate that is not prone to fading during long-term storage. • have protocols provided for both PVDF and nitrocellulose membranes. Storage Conditions: Store antibody conjugates at 4°C (undiluted). Store Western Blue® Substrate at room temperature. Protocol ProtoBlot® II AP System with Stabilized Substrate Technical Manual Western Blue® Stabilized Substrate for Alkaline Phosphatase Product Western Blue® Stabilized Substrate for Alkaline Phosphatase For Research Use Only. Not for Use in Diagnostic Procedures. For additional information see page 12. TMB Stabilized Substrate for Horseradish Peroxidase Product TMB Stabilized Substrate for Horseradish Peroxidase For Research Use Only. Not for Use in Diagnostic Procedures. For additional information see page 10. Size Cat.# Price (Fr) 200 ml W4121 221.00 Size Cat.# Price (Fr) 100 ml S3841 92.00 Part# TM026 Size Cat.# Price (Fr) 1 each W3940 471.00 1 each W3950 471.00 1 each W3960 471.00 BCIP/NBT Color Development Substrate (5-bromo-4-chloro-3-indolyl-phosphate/nitro blue tetrazolium) Product BCIP/NBT Color Development Substrate For Laboratory Use. For additional information see page 4. Size Cat.# Price (Fr) 1.25/2.5 ml S3771 126.00 13 For complete and up-to-date product information visit: www.promega.com/catalog 241 Protein Expression and Analysis Pagina 244
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