Science Catalog 2012 Life Cell Signaling Transcend™ Non-Radioactive Translation Detection Systems Product Transcend™ Colorimetric Translation Detection System Transcend™ Chemiluminescent Translation Detection System Available Separately Transcend™ tRNA For Research Use Only. Not for Use in Diagnostic Procedures. Description: The Transcend™ Non-Radioactive Translation Detection Systems allow non-radioactive detection of proteins synthesized in vitro. Using these systems, biotinylated lysine residues are incorporated into nascent proteins during translation, eliminating the need for labeling with [35S] methionine or other radioactive amino acids. This biotinylated lysine is added to the translation reaction as a precharged ε-labeled biotinylated lysine-tRNA complex (Transcend™ tRNA) rather than a free amino acid. After SDS-PAGE and electroblotting, the biotinylated proteins can be visualized by binding either Streptavidin-Alkaline Phosphatase (Streptavidin-AP) or Streptavidin-Horseradish Peroxidase (Streptavidin-HRP), followed either by colorimetric or chemiluminescent detection. Typically, these methods can detect 0.5–5ng of protein within 3–4 hours after gel electrophoresis. This sensitivity is equivalent to that achieved with [35S]methionine incorporation and autoradiographic detection 6–12 hours after gel electrophoresis. Features: • Sensitive: The biotin tag allows detection of 0.5–5ng of translated protein. • Safe: No radioisotope handling, storage or disposal is required. • Fast: Labeled proteins can be detected 3–4 hours after gel electrophoresis. • Flexible: Results can be visualized by using colorimetric or chemiluminescent detection. Storage Conditions: Store Transcend™ tRNA at –70°C. Do not subject the Transcend™ tRNA to more than five freeze-thaw cycles. Store all other components at 4°C. Protocol Transcend™ Non-Radioactive Translation Detection System Technical Bulletin ECL Western Blotting Substrate Product ECL Western Blotting Substrate For Research Use Only. Not for Use in Diagnostic Procedures. Description: The ECL Western Blotting Substrate is a highly sensitive non-radioactive, enhanced luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP) on immunoblots. The ECL Western Blotting Substrate detects picogram amounts of antigen, and with the use of photographic or other imaging methods, visualizes the presence of HRP. Features: • High Sensitivity: Detect picogram levels of protein. • Save Time: No optimization required; you can switch from other entrylevel ECL substrates. Storage Conditions: Store at 2–8°C. Protocol ECL Western Blotting Substrate Technical Manual Part# TM317 Size Cat.# Price (Fr) 250 ml W1001 233.00 500 ml W1015 327.00 Part# TB182 Blocking Agents Product Blot-Qualified BSA Tween® 20 For Laboratory Use. Description: This BSA (bovine serum albumin) has been tested and qualified for optimum performance in immunoblotting applications with alkaline phosphatase antibody conjugates. It is shown to be alkaline phosphatase-free. Tween® 20 is a nonionic detergent used as a buffer component for immunoscreening in the ProtoBlot® Systems. In addition to blocking agents such as BSA, which saturate excess sites of antibody binding on membranes, this detergent acts in solution to dissociate nonspecific interactions with an antibody probe. Size Cat.# Price (Fr) 10 g W3841 97.00 2.5 ml W3831 59.00 Size Cat.# Price (Fr) 30 reactions L5070 402.00 30 reactions L5080 589.00 30 µl L5061 308.00 Product AttoPhos® AP Fluorescent Substrate System Size Cat.# Price (Fr) AttoPhos® AP Fluorescent Substrate System 3 × 36 mg S1000 613.00 AttoPhos® AP Fluorescent Substrate System Trial Size Available Separately AttoPhos® Substrate AttoPhos® Buffer For Research Use Only. Not for Use in Diagnostic Procedures. Description: AttoPhos® AP Fluorescent Substrate System contains a highly sensitive fluorescent alkaline phosphatase (AP) substrate. Features: • Sensitivity: Low fluorescence signal until enzymatically acted upon, yielding detection of AP to 0.1 attomole. • Low Background: Low fluorescence from interfering biological molecules. • Linearity: Linear kinetics over five orders of magnitude of AP concentration. • Additional Features: Excitation at 435nm, emission at 555nm and large Stokes’ shift (≈120nm). Storage Conditions: Store at 4°C. Protocol AttoPhos® AP Fluorescent Substrate System Technical Bulletin Part# TB280 1 × 36 mg S1001 215.00 36 mg S1011 187.00 100 mg S1012 390.00 1 g S1013 1950.00 60 ml S1021 99.00 240 ml S1022 238.00 TMB One Solution Product TMB One Solution For Laboratory Use. For additional information see page 176. Size Cat.# Price (Fr) 100 ml G7431 139.00 240 For complete and up-to-date product information visit: www.promega.com/catalog Pagina 243
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