Cell Signaling Inducible T7-Driven Flexi® Vectors for E. coli Expression Product pF1A T7 Flexi® Vector pF1K T7 Flexi® Vector pFN18A HaloTag® T7 Flexi® Vector pFN18K HaloTag® T7 Flexi® Vector pFN19A HaloTag® T7 SP6 Flexi® Vector pFN19K HaloTag® T7 SP6 Flexi® Vector pFC20A HaloTag® T7 SP6 Flexi® Vector pFC20K HaloTag® T7 SP6 Flexi® Vector pFN2A (GST) Flexi® Vector pFN2K (GST) Flexi® Vector pFN6A (HQ) Flexi® Vector pFN6K (HQ) Flexi® Vector pFC7A (HQ) Flexi® Vector pFC7K (HQ) Flexi® Vector For Research Use Only. Not for Use in Diagnostic Procedures. Description: These Flexi® vectors are designed specifically for expression of proteins in E. coli from the T7 RNA polymerase promoter with or without the addition of purification tags. Expression levels can vary from protein to protein, but in general the N-terminal HaloTag® fusion protein (e.g., pFN18A/K) can increase expression by increasing solubility of the expressed protein. Proteins may be purified using the HaloTag® fusion with the HaloTag® Protein Purification System (Cat.# G6280). Expression may be confirmed through cell-free expression with the S30 T7 High-Yield Protein Expression System (Cat.# L1110). Note: Flexi® Vectors contain the lethal barnase gene to reduce background colonies without inserts during the subcloning procedure. The Flexi® Vector Cloning System replaces the barnase gene with your insert. These vectors, as purchased, cannot be cultured in normal laboratory strains of E. coli without an insert. Features: • Versatility: You can choose between a variety of initial applications (e.g., bacterial protein, mammalian, or cell-free protein expression) and then transfer to others as required. • Time Savings: Efficient transfer allows for direct use of recombinant clones, minimizing time wasted screening background colonies. • Enhanced Productivity: Adaptable to high-throughput formats for large screening projects. • Easy Access: No licensing fees or complicated transfer restrictions. Storage Conditions: Store vectors at –20°C. Size Cat.# Price (Fr) 20 µg C8441 424.00 20 µg C8451 424.00 20 µg G2751 399.00 20 µg G2681 399.00 20 µg G1891 399.00 20 µg G1841 399.00 20 µg G1681 399.00 20 µg G1691 399.00 20 µg C8461 476.00 20 µg C8471 476.00 20 µg C8511 476.00 20 µg C8521 476.00 20 µg C8531 476.00 20 µg C8541 476.00 FluoroTect™ GreenLys in vitro Translation Labeling System Product FluoroTect™ GreenLys in vitro Translation Labeling System For Research Use Only. Not for Use in Diagnostic Procedures. Description: The FluoroTect™ GreenLys in vitro Translation Labeling System allows for the fluorescent labeling and detection of proteins synthesized in vitro. The system is based on a lysine-charged tRNA that is labeled at the ε position of the lysine with the fluorophore BODIPY®-FL. Fluorescent lysine residues will be incorporated into synthesized proteins during in vitro translation reactions, eliminating the need for radioactivity. Detection of the labeled proteins is accomplished in 2–5 minutes directly “in-gel” by use of a laser-based fluorescent gel scanner. This eliminates any requirements for protein gel manipulation such as fixing/drying or any safety, regulatory and waste disposal issues associated with the use of radioactively labeled amino acids use. The convenience of “in-gel” detection also avoids the time-consuming electroblotting and detection steps of conventional nonisotopic systems. Features: • Fast: Data can be obtained in minutes, eliminating overnight exposures associated with radioactive-based systems or time-consuming steps utilized by traditional non-isotopic methodologies. • Convenient: Results based on “in-gel” detection. No requirement to transfer, fix, or dry gels. • Non-Radioactive: No safety, regulatory or waste disposal issues associated with radioactivity. • Flexible: The modified charged tRNA can be used with a variety of Promega translation systems including: Rabbit Reticulocyte Lysate, TNT® Coupled Transcription/Translation System, Wheat Germ Extract and E. coli S30 Extract. Storage Conditions: Store at –70°C. Protocol FluoroTect™ GreenLys in vitro Translation Labeling System Technical Bulletin Part# TB285 Size Cat.# Price (Fr) 40 reactions L5001 790.00 13 For complete and up-to-date product information visit: www.promega.com/catalog 239 Protein Expression and Analysis Pagina 242
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