Cell Signaling Bright-Glo™ Luciferase Assay System Product Bright-Glo™ Luciferase Assay System For Laboratory Use. Description: High-throughput quantitation of firefly (Photinus pyralis) luciferase expression in mammalian cells requires highly sensitive reagents that can adapt to continuous-process robotic systems. Bright-Glo™ Luciferase Assay System is designed specifically to meet the needs of continuous-process systems by providing robust, homogeneous assay chemistry that achieves high assay sensitivity and approximately 30-minute signal half-life without prior sample processing. These attributes also benefit scientists who are using fewer samples but still require high sensitivity and ease of use. Features: • Quick: No need to remove culture medium or wash cells prior to adding assay reagent. Cells may be grown and assayed directly within the same multiwell plate. • Sensitive: Up to tenfold more light intensity than other homogeneous luciferase assay reagents results in increased sensitivity. • Improved Assay Precision: Less sensitive to mixing conditions, sample evaporation and pipetting errors, resulting in improved assay reproducibility. • Convenient: Simply mix buffer with lyophilized substrate and add to cells in culture medium; no need for thawing or measuring before use. • Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/myway/ Storage Conditions: Store Bright-Glo™ Luciferase Assay Substrate at –20°C. Store Bright-Glo™ Luciferase Assay Buffer below 25°C. Protocol Bright-Glo™ Luciferase Assay System Technical Manual Glo Lysis Buffer, 1X Product Glo Lysis Buffer, 1X For Research Use Only. Not for Use in Diagnostic Procedures. Description: Glo Lysis Buffer (GLB), 1X, is a proprietary formulation developed to promote rapid lysis (within 5 minutes) of cultured mammalian cells without scraping or performing freeze-thaw cycles. It is fully compatible with BrightGlo™ and Steady-Glo® Assay Reagents and the Luciferase Assay Reagent for analysis of firefly luciferase expression. The half-life of these reagents remains the same with or without use of GLB, >5 hours for Steady-Glo® Reagent and >24 minutes for Bright-Glo™ Reagent. Features: • Convenient: No need for cell scraping or freeze-thaw cycles. • Fast: Cell lysis within 5 minutes. • Versatile: Use with Bright-Glo™ and Steady-Glo® Reagents to provide nonhomogeneous assay formats or with other reporter applications. • Robust: Firefly luciferase enzyme in Glo Lysis Buffer is stable at room temperature for at least 48 hours. Storage Conditions: Store Glo Lysis Buffer at 4°C. For long-term storage, Glo Lysis Buffer can be frozen at –20°C or –70°C. Protocol Glo Lysis Buffer Protocol Part# 9PIE266 Size Cat.# Price (Fr) 100 ml E2661 120.00 Part# TM052 Size Cat.# Price (Fr) 10 ml E2610 181.00 100 ml E2620 1267.00 10 × 100 ml E2650 Pls. Enq. Luciferase Assay System Product Luciferase Assay System Luciferase Assay System with Reporter Lysis Buffer Luciferase Assay System, 10-Pack Luciferase Assay System Freezer Pack Luciferase 1000 Assay System Luciferase Assay Reagent Available Separately Luciferase Cell Culture Lysis 5X Reagent Reporter Lysis 5X Buffer For Research Use Only. Not for Use in Diagnostic Procedures. Description: The Luciferase Assay System is an extremely sensitive and rapid reagent for quantitation of firefly luciferase. Linear results are seen over at least eight orders of magnitude of enzyme concentration, and patented technology incorporated in the formulation has allowed for less than 10–20 moles of luciferase to be measured under optimal conditions. Generally, 100-fold greater sensitivity can be achieved over the chloramphenicol acetyltransferase (CAT) assay. The Luciferase Assay Reagent generates light that is nearly constant for at least 1 minute and so is compatible with measuring firefly luciferase in a single-tube luminometer or in a multiwell plate luminometer with an auto-injector. The Luciferase Assay System is a nonhomogeneous assay system; the cells containing the luciferase must be lysed before reagent addition. Glo Lysis Buffer (Cat.# E2661), Cell Culture Lysis Reagent (Cat.# E1531), Passive Lysis Buffer (Cat.# E1941) and Reporter Lysis Buffer (Cat.# E3971) may be used with the Luciferase Assay System for reporter quantitation in mammalian cells. The Luciferase Assay System may also be used for quantitation in plant and bacterial cells, but only Cell Culture Lysis Reagent is suitable for these applications. Reporter Lysis Buffer allows for firefly luciferase, CAT and β-galactosidase assays to be performed from the same cell extract. In some kits the lysis buffer is included, and in others it must be purchased separately (see Component Listing link above). Features: • Linear: Eight or more orders of magnitude of enzyme concentration. • Sensitive: To 10–20 moles of luciferase. • Fast: Perform cell lysis, sample preparation and assays in as little as 5 minutes. • Convenient: Reporter Lysis Buffer allows luciferase, CAT and β-galactosidase assays to be performed from the same cell extract. • Simple Assay Procedure: Eliminates the need for autoinjection devices and rapid mixing protocols when using single-tube luminometers. • Versatile: Luminometer preferred, but not required; adaptable to scintillation counters. • Safe: Non-radioactive. • Superior: High performance compared to competitors’ luciferase assays. Storage Conditions: Store system at –20°C. Store Cat.# E1483 at –70°C. Reporter Lysis Buffer (Cat.# E3971) may be stored at room temperature. Store Cat.# E2661 at 4°C. For long-term storage, Cat.# E2661 can be frozen at –20°C or –70°C. Protocol Luciferase Assay System Technical Bulletin Part# TB281 Size Cat.# Price (Fr) 100 assays E1500 167.00 100 assays E4030 167.00 1,000 assays E1501 1253.00 1,000 assays E4530 1467.00 1,000 assays E4550 1086.00 100 ml E1483 1086.00 30 ml E1531 95.00 30 ml E3971 95.00 15 For complete and up-to-date product information visit: www.promega.com/catalog 265 Reporter Assays and Transfection Pagina 268
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